Effect of insulin on the metabolism of L-ascorbic acid in animals

Majumder, Prabir K.; Banerjee, S. K.; Roy, Rohan Basu; Chatterjee, Gaurav

doi:10.1016/0006-2952(73)90407-3

Cited by 12 publications

(4 citation statements)

References 8 publications

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“…The decrease in ascorbic acid (AA) and a-tocopherol levels in STZ sperm indicate increased degradation and reduced biosynthesis (Majumder et al, 1972). The decreased GLO activity impairs the ability to synthesize AA (Bode and Yavarow, 1993) or increase the urine excretion and oxidative loss (Kashiba et al, 2002) and also reduce GSH-and NADPH-dependent AA regeneration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat

Suresh

Prithiviraj

Lakshmi

et al. 2013

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat

Suresh

Prithiviraj

Lakshmi

et al. 2013

Journal of Ethnopharmacology

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“…Ascorbic acid (AA) is a naturally occurring major antioxidant, essential for the scavenging of free radicals. The activity of AA has been significantly reduced in the STZ rat that can be due to increased degradation/decreased biosynthesis via diminished activity of L‐gulono‐gamma‐lactone oxidase, a terminal enzyme of AA biosynthesis [56,57] or via increased urine excretion and oxidative loss [58]. Apart from this, the reduced levels of GSH observed in STZ rat can have declined the AA regeneration (GSH‐NADPH oxidase‐dependent) [58].…”

Section: Discussionmentioning

confidence: 99%

Effect ofMucuna pruriens(Linn.) on Oxidative Stress-Induced Structural Alteration of Corpus Cavernosum in Streptozotocin-Induced Diabetic Rat

Suresh

Prakash

2011

The Journal of Sexual Medicine

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Introduction Erectile dysfunction is one of the major secondary complications of diabetes. Mucuna pruriens (M. pruriens), a leguminous plant identified for its antidiabetic, aphrodisiac, and fertility enhancing properties, has been the choice of Indian traditional medicine. Aim The objective of the present study was to analyze the efficacy of M. pruriens on free radicals-mediated penile tissue alterations in hyperglycemic male rats. Methods Male albino rats were divided as group I (sham) control, group II (STZ) diabetes-induced (streptozotocin 60 mg/kg of body weight [bw] in 0.1 M citrate buffer), group III (STZ + MP) diabetic rats administered with 200 mg/kg bw of ethanolic extract of M. pruriens seed, group IV (STZ + SIL) diabetic rats administered with 5 mg/kg bw of sildenafil citrate, group V (sham + MP) administered with 200 mg/kg bw of extract alone, and group VI (sham + SIL) administered with 5 mg/kg bw of sildenafil citrate. The M. pruriens and sildenafil citrate were given (gavage) once daily for a period of 60 days. At the end of 60 days, the animals were sacrificed and subjected to analysis of reactive oxygen species levels, enzymic and nonenzymic antioxidant levels, levels of NOx, histological, and histomorphometrical study of penile tissue. Main Outcome Measures Remedial use of M. pruriens seed extract on diabetes-induced erectile tissue damage. Results Significantly high levels of oxidative stress and low levels of antioxidants in the penile tissue seem to contribute to the increased collagen deposition and fibrosis of erectile tissue in STZ rats. Relatively, there was increased damage in STZ + SIL group. Supplementation of M. pruriens in STZ + MP group has revealed the potency to overcome oxidative stress, and good preservation of penile histoarchitecture. Conclusion The ethanolic extract of M. pruriens seed significantly recovered or protected erectile tissue from the oxidative stress-induced degeneration by its antioxidant potentials. These findings propound to serve mankind by the treatment of diabetes-induced erectile dysfunction.

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“…Therefore oxidized metabolites of AA should be reductively regenerated from MDAA and DAA particularly in humans and some mammals that lack the ability to synthesize AA [8]. Because AA synthesis in most animals is restricted to the liver [9][10][11], AA, MDAA and DAA should be metabolized via interorgan co-operation to maintain steady-state levels of AA in plasma and tissues [7,12]. Concentrations of AA in cells and tissues are higher than those in plasma by ∼ 2 orders of magnitude.…”

Section: Introductionmentioning

confidence: 99%

Dynamic aspects of ascorbic acid metabolism in the circulation: analysis by ascorbate oxidase with a prolonged in vivo half-life

Kasahara

Kashiba

Jikumaru

et al. 2009

Biochemical Journal

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Because AA (L-ascorbic acid) scavenges various types of free radicals to form MDAA (monodehydroascorbic acid) and DAA (dehydroascorbic acid), its regeneration from the oxidized metabolites is critically important for humans and other animals that lack the ability to synthesize this antioxidant. To study the dynamic aspects of AA metabolism in the circulation, a long acting AOase (ascorbate oxidase) derivative was synthesized by covalently linking PEG [poly(ethylene glycol)] to the enzyme. Fairly low concentrations of the modified enzyme (PEG-AOase) rapidly decreased AA levels in isolated fresh plasma and blood samples with a concomitant increase in their levels of MDAA and DAA. In contrast, relatively high doses of PEG-AOase were required to decrease the circulating plasma AA levels of both normal rats and ODS (osteogenic disorder Shionogi) rats that lack the ability to synthesize AA. Administration of 50 units of PEG-AOase/kg of body weight rapidly decreased AA levels in plasma and the kidney without affecting the levels in other tissues, such as the liver, brain, lung, adrenal grand and skeletal muscles. PEG-AOase slightly, but significantly, decreased glutathione (GSH) levels in the liver without affecting those in other tissues. Suppression of hepatic synthesis of GSH by administration of BSO [L-buthionin-(S,R)-sulfoximine] enhanced the PEG-AOase-induced decrease in plasma AA levels. These and other results suggest that the circulating AA is reductively regenerated from MDAA extremely rapidly and that hepatic GSH plays important roles in the regeneration of this antioxidant.

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Effect of insulin on the metabolism of L-ascorbic acid in animals

Cited by 12 publications

References 8 publications

Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat

Effect of Mucuna pruriens (Linn.) on mitochondrial dysfunction and DNA damage in epididymal sperm of streptozotocin induced diabetic rat

Effect ofMucuna pruriens(Linn.) on Oxidative Stress-Induced Structural Alteration of Corpus Cavernosum in Streptozotocin-Induced Diabetic Rat

Dynamic aspects of ascorbic acid metabolism in the circulation: analysis by ascorbate oxidase with a prolonged in vivo half-life

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