Effect of intensity-modulated radiation therapy on sciatic nerve injury caused by echinococcosis

Xu, Weijuan; Aikeremu, Dilimulati; Sun, Jungang; Zhang, Yanjun; Xu, Jiangbo; Zhou, Wenzheng; Zhao, Xi-Bin; Wang, Hao; Ye, Hong

doi:10.4103/1673-5374.293153

Cited by 9 publications

(8 citation statements)

References 41 publications

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“…The efficacy and safety of single ABZ-CS-MPs or IMRT have been verified before ( 13 , 19 ). In this study, lower dose and radiation intensity were selected and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed to further test the safety of this combination therapy.…”

Section: Resultsmentioning

confidence: 77%

“…One exciting aspect of this study is that we carried out IMRT in our in vivo and in vitro experiments, which improves the accuracy of the implementation process of the radiotherapy program and maximizes the irradiation of CE and, therefore, best protects the normal tissues around the cysts ( 24 ). A latest study has shown that IMRT (60 Gy) has the same antiechinococcus effect as conventional radiotherapy in vivo , and little damage was found to the sciatic nerve, fully demonstrating its validity and safety ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

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In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis

Wang

et al. 2021

Antimicrob Agents Chemother

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Currently, there is a lack of clinically safe and effective treatment for spinal cystic echinococcosis (CE). Recent studies have shown that albendazole chitosan microspheres (ABZ-CS-MPS) and irradiation have certain anti-abdominal echinococcosis ability, so this study aims to compare the in vitro and in vivo therapeutic effects of ABZ-CS-MPs, Intensity-modulated radiation therapy (IMRT) and the combination therapy on spinal echinococcosis. Firstly, protoscolices were processed by different treatments to evaluate their respective anti-echinococcosis effect by monitoring the viability change of protoscoleces. Then, the apoptotic status of protoscoleces was evaluated by detecting the changes of mitochondrial membrane potential, the expression of apoptosis proteins and the ultrastructural alterations of protoscoleces. After that, we constructed a gerbil model of spinal CE and further applied B-ultrasound and magnetic resonance imaging technology to assess the size of hydatid in vivo. Finally, the cysts were obtained and weighed to compare the inhibition rate in different groups. The combined therapy increased protoscoleces mortality to over 90% after 18 days, which showed the highest scolicidal effect. Moreover, confocal imaging, expression of apoptotic proteins and ultrastructural changes of protoscoleces showed the highest apoptotic rate in this group. In vivo, the combination treatment also exhibited the highest cyst inhibition rate (61.4%). In conclusion, our results showed that ABZ-CS-MPs combined with IMRT could be a new treatment option for spinal CE. We also provided a method to evaluate the growth and metastasis of hydatid in animals with B-ultrasound and MRI imaging technologies.

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Section: Resultsmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis

Wang

et al. 2021

Antimicrob Agents Chemother

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“…The radiation-induced neuropathy of the sciatic nerve has reported the presence of tissue apoptosis and confirmed dysregulation of Bcl-2 and Bax expression in sciatic nerve tissue [ 173 , 174 , 175 ]. In addition, the cisplatin-resistant ovarian cancer cell line model also exhibited apoptosis via intrinsic and extrinsic mechanisms incorporating p53 alterations [ 176 ].…”

Section: Resultsmentioning

confidence: 97%

Sustainable Release of Propranolol Hydrochloride Laden with Biconjugated-Ufasomes Chitosan Hydrogel Attenuates Cisplatin-Induced Sciatic Nerve Damage in In Vitro/In Vivo Evaluation

et al. 2022

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Peripheral nerve injuries significantly impact patients’ quality of life and poor functional recovery. Chitosan–ufasomes (CTS–UFAs) exhibit biomimetic features, making them a viable choice for developing novel transdermal delivery for neural repair. This study aimed to investigate the role of CTS–UFAs loaded with the propranolol HCl (PRO) as a model drug in enhancing sciatica in cisplatin-induced sciatic nerve damage in rats. Hence, PRO–UFAs were primed, embedding either span 20 or 60 together with oleic acid and cholesterol using a thin-film hydration process based on full factorial design (24). The influence of formulation factors on UFAs’ physicochemical characteristics and the optimum formulation selection were investigated using Design-Expert® software. Based on the optimal UFA formulation, PRO–CTS–UFAs were constructed and characterized using transmission electron microscopy, stability studies, and ex vivo permeation. In vivo trials on rats with a sciatic nerve injury tested the efficacy of PRO–CTS–UFA and PRO–UFA transdermal hydrogels, PRO solution, compared to normal rats. Additionally, oxidative stress and specific apoptotic biomarkers were assessed, supported by a sciatic nerve histopathological study. PRO–UFAs and PRO–CTS–UFAs disclosed entrapment efficiency of 82.72 ± 2.33% and 85.32 ± 2.65%, a particle size of 317.22 ± 6.43 and 336.12 ± 4.9 nm, ζ potential of −62.06 ± 0.07 and 65.24 ± 0.10 mV, and accumulatively released 70.95 ± 8.14% and 64.03 ± 1.9% PRO within 6 h, respectively. Moreover, PRO–CTS–UFAs significantly restored sciatic nerve structure, inhibited the cisplatin-dependent increase in peripheral myelin 22 gene expression and MDA levels, and further re-established sciatic nerve GSH and CAT content. Furthermore, they elicited MBP re-expression, BCL-2 mild expression, and inhibited TNF-α expression. Briefly, our findings proposed that CTS–UFAs are promising to enhance PRO transdermal delivery to manage sciatic nerve damage.

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“…In particular, low doses of radiation induce modifications of intracellular molecules, leading to effects on oxidation, inflammation, apoptosis, and gene expression. It has been reported that there is also a bystander effect, in which information can be transmitted from exposed cells to unexposed cells, transferring radiation damage to these unexposed cells [ 33 ], as well as an abscopal effect, in which the local radiation therapy of a tumor can also shrink distant untreated tumors [ 34 ]. The involvement of radiation in cellular responses and the immune system has also been considered.…”

Section: Biological Effects Of Radiationmentioning

confidence: 99%

Molecular Hydrogen as a Potential Clinically Applicable Radioprotective Agent

Hirano¹,

Ichikawa²,

Sato³

et al. 2021

IJMS

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Although ionizing radiation (radiation) is commonly used for medical diagnosis and cancer treatment, radiation-induced damages cannot be avoided. Such damages can be classified into direct and indirect damages, caused by the direct absorption of radiation energy into DNA and by free radicals, such as hydroxyl radicals (•OH), generated in the process of water radiolysis. More specifically, radiation damage concerns not only direct damages to DNA, but also secondary damages to non-DNA targets, because low-dose radiation damage is mainly caused by these indirect effects. Molecular hydrogen (H2) has the potential to be a radioprotective agent because it can selectively scavenge •OH, a reactive oxygen species with strong oxidizing power. Animal experiments and clinical trials have reported that H2 exhibits a highly safe radioprotective effect. This paper reviews previously reported radioprotective effects of H2 and discusses the mechanisms of H2, not only as an antioxidant, but also in intracellular responses including anti-inflammation, anti-apoptosis, and the regulation of gene expression. In doing so, we demonstrate the prospects of H2 as a novel and clinically applicable radioprotective agent.

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Effect of intensity-modulated radiation therapy on sciatic nerve injury caused by echinococcosis

Cited by 9 publications

References 41 publications

In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis

In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis

Sustainable Release of Propranolol Hydrochloride Laden with Biconjugated-Ufasomes Chitosan Hydrogel Attenuates Cisplatin-Induced Sciatic Nerve Damage in In Vitro/In Vivo Evaluation

Molecular Hydrogen as a Potential Clinically Applicable Radioprotective Agent

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