Effect of intermittent and continuous exposure to electromagnetic fields on cultured hippocampal cells

Boland, André; Delapierre, D.; Mossay, Danielle; Dresse, A; Seutin, Vincent

doi:10.1002/bem.102

Cited by 21 publications

(10 citation statements)

References 63 publications

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“…In this study, we also compared the effects of continuous and intermittent exposure to 50 Hz ELF-MFs. Boland et al (2002) reported that 50 Hz intermittent exposure at high intensities enhanced NO-induced oxidative cell damage in brain cell culture more than continuous exposure did. However, the exposure protocols used in this study show that biochemical parameters were almost equally affected by continuous and intermittent exposures.…”

Section: Discussionmentioning

confidence: 98%

ELF-EMF'in Beyin, Karaciğer ve Kalp Dokularında Oksidatif Parametreler Üzerine Etkilerinin Değerlendirilmesi

Peker

Balabanli²,

Tomruk³

et al. 2019

Karadeniz Fen Bilimleri Dergisi

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The aim of the present study was to investigate the prospective effects of continuous and intermittent extremely lowfrequency magnetic fields (ELF-EMFs) on the levels of malondialdehyde (MDA), nitric oxide (NO) and glutathione (GSH) in brain, heart, and liver tissues. The experiments performed on 18 guinea pigs were divided into three groups (n= 6 for each group). 50 Hz of 1.5 mT a magnetic field was used for exposure. Experimental groups were exposed for 4 h/day either continuously or intermittently (2h on/ 2h off/ 2h on) EMF for a period of 7 days. MDA, NO and GSH levels were determined by spectrophotometric methods. The intermittent exposure was increased GSH levels whereas that was reduced MDA levels in the brain when compared to control and continuous exposure groups. GSH concentrations of both experimental groups were found to be elevated in the liver. In the liver and heart, NO levels were increased by the continuous exposure in both administration groups, however, intermittent exposure has reduced NO levels in the both of the experimental groups. Results of this study indicated that the responses of various tissues to magnetic field exposures differed according to intensity and exposure duration of magnetic fields. ÖzBu çalışmanın amacı, sürekli ve kesikli aşırı düşük frekanslı manyetik alanların (ELF-EMF) beyin, karaciğer ve kalp dokularında; malondialdehit (MDA), nitrik oksit (NO) ve glutatyon (GSH) seviyeleri üzerindeki muhtemel etkilerini araştırmaktır. 18 gine domuzu üzerinde yapılan deneyler için hayvanlar üç gruba ayrılmıştır (her grup için n = 6). Deney grupları sürekli (4 saat / gün) veya kesikli olarak (2 saat açık / 2 saat kapalı / 2 saat açık) 7 gün boyunca 1.5 Hz frekansında ve 50 mT şiddetinde EMF'ye maruz bırakılmıştır. Dokulardaki MDA, NO ve GSH düzeyleri spektrofotometrik yöntemlerle belirlenmiştir. Kesikli maruziyet; kontrol ve sürekli maruziyet gruplarına kıyasla beyinde GSH seviyelerini arttırırken, MDA seviyelerini düşürmüştür. Her iki maruziyet grubunun GSH konsantrasyonlarının karaciğerde yükseldiği bulunmuştur. Karaciğerde ve kalpte, her iki uygulama grubunda da sürekli maruz kalma ile NO seviyeleri artmış, ancak kesikli maruziyet her iki deney grubunda da NO düzeylerini azaltmıştır. Bu çalışmanın sonuçları, çeşitli dokuların manyetik alan maruziyetlerine tepkilerinin, manyetik alanların yoğunluğuna ve maruz kalma süresine göre değiştiğini göstermiştir.

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Section: Discussionmentioning

confidence: 98%

ELF-EMF'in Beyin, Karaciğer ve Kalp Dokularında Oksidatif Parametreler Üzerine Etkilerinin Değerlendirilmesi

Peker

Balabanli²,

Tomruk³

et al. 2019

Karadeniz Fen Bilimleri Dergisi

View full text Add to dashboard Cite

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“…While some researchers have reported the existence of potentially dangerous interactions of MF with living organisms [Luben et al, 1982;Adair, 1994;Wertheimer et al, 1995;Ji et al, 1998;Kavet et al, 2000;Boland et al, 2002;Robison et al, 2002;Caprani et al, 2004;Rajendra et al, 2004], other researchers have witnessed no effect [Jackson, 1992;Cantoni et al, 1996;Fanelli et al, 1999;Sweeney et al, 2001;Jenkins et al, 2002;Kainz et al, 2003;Kurokawa et al, 2003;Tripp et al, 2003;Burchard et al, 2004;Moon-Koo et al, 2004]. To evaluate the potential effects of MF, various durations and intensities of field exposure have been studied, as well as different model systems and assay methods.…”

Section: Introductionmentioning

confidence: 97%

14.6 mT ELF magnetic field exposure yields no DNA breaks in model systemSalmonella, but provides evidence of heat stress protection

Williams

Ingebretsen

Dawson

2006

Bioelectromagnetics

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In this study, we demonstrate that common extremely low frequency magnetic field (MF) exposure does not cause DNA breaks in this Salmonella test system. The data does, however, provide evidence that MF exposure induces protection from heat stress. Bacterial cultures were exposed to MF (14.6 mT 60 Hz field, cycled 5 min on, 10 min off for 4 h) and a temperature-matched control. Double- and single-stranded DNA breaks were assayed using a recombination event counter. After MF or control exposure they were grown on indicator plates from which recombination events can be quantified and the frequency of DNA strand breaks deduced. The effect of MF was also monitored using a recombination-deficient mutant (recA). The results showed no significant increase in recombination events and strand breaks due to MF. Evidence of heat stress protection was determined using a cell viability assay that compared the survival rates of MF exposed and control cells after the administration of a 10 min 53 degrees C heat stress. The control cells exhibited nine times more cell mortality than the MF exposed cells. This Salmonella system provides many mutants and genetic tools for further investigation of this phenomenon.

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“…Living organisms are electrochemical systems that use ELF-EMFs from the structure of proteins to cell-to-cell communication and nervous system functions. The widespread use of electricity increases the potential sources of radiation resulting in continuous and/or intermittent exposure of living beings to ELF-EMFs (Boland et al, 2002). Magnetic flux densities of EMFs of the devices offered by the technology, which make our life easier, vary between 1 × 10 −4 milli Tesla (mT) and 2.5 mT, and this value, which is more than magnetic flux densities of the EMF of human body (10 −7 mT) and natural environment (10 −6 mT) can disrupt the harmony (Seyhan, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats

et al. 2020

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The aim of the present study was to systematically investigate the effects of chronic exposure to extremely low-frequency electromagnetic field (ELF-EMF) on electrophysiological, histological and biochemical properties of the diaphragm muscle in rats. Twenty-nine newly weaned (24 days old, 23–80 g) female ( n = 15) and male ( n = 14) Wistar Albino rats were used in this study. The animals were randomly divided into two groups: the control group and the electromagnetic field (EMF) group. The control group was also randomly divided into two groups: the control female group and the control male group. The EMF exposure group was also randomly divided into two groups: the ELF-EMF female group and the ELF-EMF male group. The rats in the ELF-EMF groups were exposed for 4 h daily for up to 7 months to 50 Hz frequency, 1.5 mT magnetic flux density. Under these experimental conditions, electrophysiological parameters (muscle bioelectrical activity parameters: intracellular action potential and resting membrane potential and muscle mechanical activity parameter: force–frequency relationship), biochemical parameters (Na+, K+, Cl− and Ca+2 levels in the blood serum of rats; Na+-K+ ATPase enzyme-specific activities in muscle tissue; and free radical metabolism in both muscle tissue and serum) and transmission electron microscopic morphometric parameters of the diaphragm muscle were determined. We found that chronic exposure to ELF-EMF had no significant effect on the histological structure and mechanical activity of the muscle and on the majority of muscle bioelectrical activity parameters, with the exception of some parameters of muscle bioelectrical activity. However, the changes in some bioelectrical activity parameters were relatively small and unlikely to be clinically relevant.

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Effect of intermittent and continuous exposure to electromagnetic fields on cultured hippocampal cells

Cited by 21 publications

References 63 publications

ELF-EMF'in Beyin, Karaciğer ve Kalp Dokularında Oksidatif Parametreler Üzerine Etkilerinin Değerlendirilmesi

ELF-EMF'in Beyin, Karaciğer ve Kalp Dokularında Oksidatif Parametreler Üzerine Etkilerinin Değerlendirilmesi

14.6 mT ELF magnetic field exposure yields no DNA breaks in model systemSalmonella, but provides evidence of heat stress protection

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats

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