Effect of Intrahypothalamic Infusion of Acetylcholine on Behavioural and Physiological Thermoregulation in the Rat

Beckman, Alexander L.; Carlisle, Harry J.

doi:10.1038/221561a0

Cited by 49 publications

(13 citation statements)

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“…Using the technique of micro-iontophoresis, Beckman & Eisenman (1970) demonstrated that the warm-responsive neurones in the hypothalamus in rat and cat which were considered to be thermodetectors were relatively insensitive to 5-HT, NA and ACh, but thermo-responsive neurones of both animals which were considered to be interneurones responded to ACh and NA. The responses of presumed interneurones in the rat may explain the results of micro-injection experiments of ACh and NA, but not of 5-HT Beckman & Carlisle, 1969;Beckman, 1970). Furthermore, the responses of interneurones in the cat conflict with the results ofinjection experiments (Feldberg & Myers, 1965).…”

Section: Discussionmentioning

confidence: 90%

“…On the other hand, in rabbits (Cooper, Cranston & Honour, 1965) and sheep (Bligh, 1966), NA produced the hyperthermia and 5-HT hypothermia. In addition, cholinergic influences on the central mechanism of thermoregulation have been suggested in the rat and the monkey (Beckman & Carlisle, 1969;Lomax, Foster & Kirkpatrick, 1969;Myers & Yaksh, 1969). These observations raise the question as to whether the amines and acetylcholine (ACh) produce thermoregulatory responses by the actions on the thermo-responsive neurones in the preoptic area and anterior hypothalamus.…”

Section: Introductionmentioning

confidence: 99%

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Effects of biogenic amines on central thermoresponsive neurones in the rabbit

Hori¹,

Nakayama²

1973

The Journal of Physiology

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SUMMARY1. Single unit activities were recorded with five-barrelled micropipettes from the thermo-responsive neurones in the preoptic area and the mid-brain reticular formation in urethanized rabbits. 5-hydroxytryptamine (5-HT), noradrenaline (NA) and acetylcholine (ACh) were applied micro-iontophoretically to the immediate vicinity of the recording cells.2. Out of seventeen warm-responsive neurones recorded in the preoptic area, fifteen neurones responded to 5-HT with the increase in firing rate and two showed no response. Thirteen out of seventeen warm-units decreased their firing rate in response to application of NA and four were not affected. ACh had no effect on any of the warm-units examined.3. Six out of seven cold-units in the preoptic area were depressed by 5-HT, while NA excited five of six units studied. None of the cold-units were influenced by ACh.4. These results are in good agreement with the changes in rectal temperature produced by 5-HT and NA micro-injected into the hypothalamus in rabbits.5. In the mid-brain reticular formation, 5-HT excited all of fourteen cold-responsive neurones. Of these, eight cold-units were depressed and six were unaffected by NA, while ACh excited six units and had no effect on eight units. All of the five warm-responsive units were inhibited by 5-HT and none were influenced by NA. Thus, the responses of reticular thermoresponsive neurones to 5-HT and NA were opposite to those of the preoptic thermo-responsive neurones.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effects of biogenic amines on central thermoresponsive neurones in the rabbit

Hori¹,

Nakayama²

1973

The Journal of Physiology

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“…The injection of atropine into the rostral hypothalamus of the rat causes a rise in rectal temperature (Kirkpatrick & Lomax, 1967) and the injection of acetylcholine into the same area causes a fall in temperature which is antagonized by atropine (Beckman & Carlisle, 1969 ;Kirkpatrick & Lomax, 1970). Anticholinesterase drugs similarly cause a fall in rectal temperature through an action in the brain (Meeter & Wolthuis, 1968;Friedman & Jaffe, 1969).…”

Section: Introductionmentioning

confidence: 99%

Inhibition by atropine of the increased turnover of noradrenaline in the hypothalamus of rats exposed to cold

Simmonds

1971

British J Pharmacology

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Summary1. Small doses of (-)-[3H] noradrenaline were injected into the lateral cerebral ventricles in rats to label radioactively the endogenous noradrenaline (NA) stores.2. Intraventricular injection of 25 1ug atropine methonitrate at the same time inhibited the increased rate of disappearance of [3H] NA from the hypothalamus at an environmental temperature of 90 C, when compared with the values at 240 C, without impairing temperature regulation. 3. At 320 C, 25 jtg atropine methonitrate caused a lethal hyperthermia. A dose of 5 yg was not lethal and did not inhibit the increased rate of disappearance of [3H] NA from the hypothalamus. 4. It is concluded that the pathway which stimulates an increased turnover of NA in the cold contains an atropine sensitive synapse but is not the principal pathway of heat production. The increased turnover of NA in the heat probably does not involve an atropine sensitive synapse.

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“…the limbic circuit [31]. As has been suggested for carbachol induced drinking there is also evidence for behavioral influences on body temperature [5,6,9]. Cholinergic stimulation of parts of the limbic circuit is known to influence the behavioral status of the animal.…”

Section: Influence Of Intracerebral Implantation Of Atropine Sulphatementioning

confidence: 93%

Intracerebral implantation of carbachol in the rat: Its effect on water intake and body temperature

Hulst

1972

Physiology & Behavior

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HULST, S. G. Th. lntracerebral implantation of carbachol in the rat: its effect on water intake and body temperature.PHYSIOL. BErL~V. 8 (5) [865][866][867][868][869][870][871][872] 1972.--Intracerebral carbachol produces a fall in body temperature as well as drinking in the rat when implanted in various subcortical structures, related to the emotion-motivation limbic circuit. These effects are due to a central cholinergic stimulation since they can be prevented by the systemic administration of the centrally active anticholinergic substance atropine sulphate and to a lesser degree by methylatropine nitrate. By withholding water during the first hr following carbachol implantation it could be shown that the hypothermic response is independent from water intake. When carbachol as well as atropine sulphate are implanted in two locafisations, which both induce hypothermia as well as drinking following carbachol stimulation, atropine sulphate nearly always blocked drinking, but practically only when atropine sulphate was applied caudally to carbachol did it block hypothermia. The results suggest a drinking and hypothermic circuit within the limbic system, anatomically linked but functionally different and independent. Water intakeBody temperature Carbachol Atropine Intracerebral implantation INTRACEREBRALLY implanted carbachol elicits vigorous drinking in the water satiated rat [16,17]. Grossman [16] and Fisher and Coury [13] found that cholinergic stimulation within perifornical region, as well as the limbic system, the hypothalamus and the midbraln induced drinking. It was postulated that the emotion-motivation limbic circuit, as proposed by Papez [29,30] is intimately involved in the mediation of drinking. Further evidence for such a circuit has been presented [12,26,27,32,33]. In the rat hypothermia has been found following cholinergic stimulation of the anterior hypothalamus, lateral hypothalamus, preoptic region, nucleus lateralis septi and the area between the thalamie nuclei and the nucleus ruber [20,22,23,25]. In a previous study [20] it was suggested that cholinergically evoked drinking and hypothermia follow roughly parallel pathways in the limbic system and the diencephalon. As the fibre systems for these two phenomena need not necessarily be the same, further experiments were conducted to evaluate the previous results; these studies are reported here. MATER/AI~ AND METHODSMale white rats of an inbred Wistar strain weighing 180-190 g were used. Crystalline substances were implanted into various subcortical structures as follows. A stainless steel plate, equipped with 12 holes, was used. Tubes, with a diameter of 0.8 ram, of the same material, protruding 2.50 mm from the lower side of the plate, were attached to the holes in the plate. This plate was fixed to the rat skull with dental cement (for details see [20]). Through the tubes needles of different length, containing crystalline substances at the tip, could be directed into the brain. The operated animals were allowed to recover from the operation and...

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Effect of Intrahypothalamic Infusion of Acetylcholine on Behavioural and Physiological Thermoregulation in the Rat

Cited by 49 publications

References 10 publications

Effects of biogenic amines on central thermoresponsive neurones in the rabbit

Effects of biogenic amines on central thermoresponsive neurones in the rabbit

Inhibition by atropine of the increased turnover of noradrenaline in the hypothalamus of rats exposed to cold

Intracerebral implantation of carbachol in the rat: Its effect on water intake and body temperature

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