Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Dawod, Ahmed; Miró, Jordi; Elbaz, Hamed; Fahmy, Hossam Mahmoud Ahmad; Abdoon, Ahmed S.

doi:10.3390/ani11041123

Cited by 13 publications

(7 citation statements)

References 35 publications

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“…Treatment with PRP is a valuable therapy for improving the embryo recovery in superovulating cows [ 14 ]. Furthermore, it has been used to increase fertility in cows with ovarian hypofunction [ 15 ] and to increase the pregnancy rate in repeat breeding of pure Arabian mares [ 16 ]. Platelet-rich plasma could modulate the inflammatory and immune activation processes and potentially minimize inflammation-associated tissue damage [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Silviu‐Ionuț¹,

Dascălu²,

Borş

et al. 2022

Animals

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The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GFequina significantly (p < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus–oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GFequina can improves OPU-IVEP results in Holstein cows.

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Section: Introductionmentioning

confidence: 99%

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Silviu‐Ionuț¹,

Dascălu²,

Borş

et al. 2022

Animals

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“…PRP is a concentration of platelets containing multiple growth factors that are very important for regeneration and antiinflammatory processes. Treatment with PRP has been used for various conditions in equine veterinary medicine (Gentile et al 2012;McCarrel et al 2012; Holmes et al After PRP treatment, irregular cartilage line was remodelled to normal shape and bone density was improved in radiology 2018; Ortved 2018;Pereira et al 2019;Machado et al 2019;Garbin and Olver 2020;Dawod et al 2021;Maleas and Mageed 2021;Ribitsch et al 2021;Perego et al 2022) and hoof disorders (Carmona et al 2013;Angelone et al 2017;Carmona et al 2018;Cengiz et al 2018;Seidel et al 2021). Renewable treatments that can replace conventional antibiotic treatments range from auto-PRP to auto-stem cells (Pierce et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Equine Musculoskeletal Disease using Autogenous Platelet Rich Plasma

2022

Int J Vet Sci

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This study evaluated the therapeutic effect using autogenous platelet-rich plasma (PRP) for the musculoskeletal disease of Thoroughbred horses raised in South Korea. Twenty-five Thoroughbred horses (15 adult horses more than 2 years old, 10 fostering horses less than 2 years old) were diagnosed with musculoskeletal disorders (2 horses with desmitis of the suspensory ligament, 9 horses with osteoarthritis, 14 horses with fracture) by ultrasound and X-ray were used in this study. We collected venous blood of patients by using ACP Double Syringe after sterilization of jugular site, and then autogenous PRP was taken through centrifugation. We were observed the healing process of the lesion by using ultrasonography and X-ray images after injecting 4mL of autogenous PRP into each lesion for 2-times. Results of this study, 20 (80%) of 25 damaged horses returned to normal competition. The core lesion in the ligament disappeared in 90 days and healthy tissue regenerated. The fracture line of the cortex of bone disappeared in 60 days, and no side effects such as osteophytosis during healing progress. Remodeled articular surface, and cartilage defect, a fragment in joint disappeared. It could be confirmed that applying autogenous PRP treatment is an excellent regenerative therapy for equine locomotorium lesions.

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“…In a larger study, 73 Arabian repeat-breeder mares were treated with either PRP or lyophilised growth factors from horse platelets (L-GFEquina). The length of the oestrous cycle was shortened, endometrial thickness was increased and pregnancy rate was increased in the treated mares ( 63 ).…”

Section: Treatment Optionsmentioning

confidence: 99%

A Novel Approach to Minimising Acute Equine Endometritis That May Help to Prevent the Development of the Chronic State

Morrell

Rocha

2022

Front. Vet. Sci.

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One of the most commonly encountered challenges in equine breeding is endometritis, which can be difficult to resolve and causes considerable economic losses to the industry. It is a multifactorial condition, developing as an exaggerated form of the normal physiological response to breeding. Seminal plasma proteins, spermatozoa, bacteria and debris initiate an inflammatory response; the resulting fluid and neutrophils are then cleared from the uterus along with the debris. However, in some mares, the response is prolonged or exaggerated, with much fluid formation and neutrophil infiltration leading to acute endometritis. A bacterial cause has been implicated, although in some cases no pathogenic organisms can be isolated on culture. It has been postulated that any one of a variety of bacteria could be involved, or dysbiosis of the uterine microbiome could be responsible. Repeated episodes of acute endometritis may lead to the pathology associated with chronic endometritis, with mucociliary dysfunction, vascular degeneration and plasma cell infiltration. This review examines the information that is currently available about equine endometritis, particularly about the role of the inseminate in the uterus, and its current treatment. There are some promising lines of research into treatment or prevention that may help to resolve the issue.

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Effect of Intrauterine Infusion of Equine Fresh Platelets-Rich Plasma (PRP) or Lyophilized PRP (L-GFequina) on Ovarian Activity and Pregnancy Rate in Repeat Breeder Purebred Arabian Mares

Cited by 13 publications

References 35 publications

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows

Therapeutic Effect of Equine Musculoskeletal Disease using Autogenous Platelet Rich Plasma

A Novel Approach to Minimising Acute Equine Endometritis That May Help to Prevent the Development of the Chronic State

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