Effect of ionic liquid on activity, stability, and structure of enzymes: A review

Naushad, Mu.; ALOthman, Zeid A.; Khan, Abbul Bashar; Ali, Maroof

doi:10.1016/j.ijbiomac.2012.06.020

Cited by 295 publications

(166 citation statements)

References 91 publications

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“…At exposed positions, more improved variants were obtained with charged substitutions than with polar ones (unlike the distribution in BSLA WT: more aliphatic amino acids exposed as well as buried; [52] It has often been reported that deactivation increases with increasing H-bond basicity. [22,53,54] the number of H-bonds to the enzyme backbone amides.…”

Section: Generation and Sequencing Of Ssm Libraries Of Whole Bslamentioning

confidence: 99%

Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A

et al. 2015

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Ionic liquids (ILs) are attractive (co-)solvents for biocatalysis. However, in high concentration (>10 % IL), enzymes usually show decreased activity. No general principles have been discovered to improve IL resistance of enzymes by protein engineering. We present a systematic study to elucidate general engineering principles by site saturation mutagenesis on the complete gene bsla. Screening in presence of four [BMIM]-based ILs revealed two unexpected lessons on directed evolution: 1) resistance improvement was obtainable at 50-69 % of all amino acid positions, thus explaining the success of small sized random mutant libraries; 2) 6-13 % of substitutions led to improved resistance. Among these, 66-95 % were substitutions by chemically different amino acids (e.g., aromatic to polar/aliphatic/charged amino acids), thus indicating that mutagenesis methods introducing such changes should, at least for lipases like BSLA, be favored to improve IL resistance.

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Section: Generation and Sequencing Of Ssm Libraries Of Whole Bslamentioning

confidence: 99%

Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A

et al. 2015

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“…To cope with the noticeable mass transfer problem caused by the relatively high viscosity of ILs, we tried to mix pure ILs with a typical organic solvent THF to form different IL-containing systems. As shown in Table 1 + -containing ILs indicated that the anion type of the ILs played a crucial role in whole-cell mediated acylation [12,16,22]. For example, the [PF 6 ] -, an enzyme-compatible anion, may had lower hydrogen bond basicity, thus minimizing interference with the internal hydrogen bonds of the enzyme.Although different IL-containing systems were adopted, the 5′-regioselectivity of the whole-cells remained as high as >99%.…”

Section: Resultsmentioning

confidence: 99%

Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation

et al. 2014

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Background: The use of biocatalysts has become an increasingly attractive alternative to traditional chemical methods, due to the high selectivity, mild reaction conditions and environmentally-friendly processes in nonaqueous catalysis of nucleosids. However, the extensive use of organic solvents may generally suffer from sever drawbacks such as volatileness and toxicity to the environment and lower activity of the biocatalyst. Recently, ionic liquids are considered promising solvents for nonaqueous biocatalysis of polyhydroxyl compounds as ILs are environmental-friendly.

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“…However, product recovery from ionic liquid-enzyme reaction media by another non-aqueous green solvent, such as supercritical CO 2 , is nowadays considered the most interesting strategy for developing integral clean chemical processes [34]. Many works and excellent reviews have been published during the last decade regarding the use of enzymes in ionic liquids [14][15][16][40][41][42][43][44][45][46][47]. The results showed that enzymes in ionic liquids have many advantages compared to those in organic solvents such as high activity, high selectivity including substrate, region-and enantioselectivity, better enzyme stability as well as better recoverability and recyclability.…”

Section: Enzymes In Ionic Liquidsmentioning

confidence: 99%

Compatibility of Ionic Liquids with Enzymes

Lan

Koo

2013

Production of Biofuels and Chemicals With Ionic Liquids

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The potential of ionic liquids as a green alternative to environmentally harmful volatile organic solvents has been well recognized. Being considered as "designer solvents", ionic liquids have been used extensively in a wide range of applications including biotransformations. As compared to those in traditional organic solvents, enzyme performance in ionic liquids is showed enhance in their activity, enantioselectivity, stability, as well as their recoverability and recyclability. This chapter will cover the biocompatibility issue of ionic liquids with enzymes. The effects of ionic liquid properties on the enzymatic reactions and conformation of enzyme as well as methods for activation and stabilization of enzymes in ionic liquids will be described. In addition, the current attempts for rational design of biocompatible ionic liquids will be also discussed.

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Effect of ionic liquid on activity, stability, and structure of enzymes: A review

Cited by 295 publications

References 91 publications

Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A

Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A

Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation

Compatibility of Ionic Liquids with Enzymes

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