Effect of ionotropic glutamate receptors antagonists on the modifications in extracellular glutamate and aspartate levels during picrotoxin seizures: a microdialysis study in freely moving rats

An Analytical Flow Injection System to Measure Glutamate in Microdialysis Samples Based on an Enzymatic Reaction and Electrochemical Detection

“…These data contrast with the report of a fall in extracelluar Glu concentration during seizures induced by picrotoxin administration through a dialysis probe in the hippocampus [20,21]. This could be due to the lower temporal resolution in those experiments that could mask acute changes.…”

Methotrexate induces seizure and decreases glutamate uptake in brain slices: Prevention by ionotropic glutamate receptors antagonists and adenosine

“…DNQX was demonstrated to be effective against seizures induced by electroshock or pentylenetetrazol, and also by picrotoxin (Sierra-Paredes et al, 2000). As observed with MK-801, the decrease in glutamatergic receptors stimuli could be implicated in the DNQX neuroprotective effect.…”

Simultaneous glutamate and EEG activity measurements during seizures in rat hippocampal region with the use of an electrochemical biosensor

“…In general, glutamate concentrations tended to be higher once seizure activity initiated, but it was at the initiation of hippocampal status epilepticus, and at the termination of the experiments with euthanasia that the clearest evidence of glutamate release occurred. Sierra-Paredes et al (2000, 2001 have reported an extracellular glutamate decrease during seizures in a model of epilepsy induced by administration of picrotoxin into the hippocampus through a microdialysis probe. These findings contrast with the results obtained in the present study due to the different technique used to measure glutamate concentrations, that is, sampling intervals of long duration reduce temporal resolution, and can mask acute changes in synaptic or extracellular glutamate concentration measured with microdialysis methods.…”