Effect of Iron Supplementation on Oxidative Stress and Antioxidant Status in Iron-Deficiency Anemia

Abstract

This study was designed to measure the effect of iron supplementation on antioxidant status in iron-deficient anemia, including the time for hemoglobin normalization and at the time of filling of iron body stores. The extent of plasma lipid peroxidation was evaluated by measuring the levels of malondialdehyde and glutathione peroxidase (GSH-Px), and the activities of superoxide dismutase (SOD) and catalase in 63 patients with iron-deficiency anemia before and after 6 wk of iron supplementation and at the time when body iron stores are saturated. After 6 wk of iron supplementation, a significant decrease of oxidative stress was observed in the treated subjects relative to controls (p<0.05). No significant differences existed between treated patients at 6 wk and at the end of the study. The erythrocyte levels of catalase, SOD, and GSH-Px were significantly lower in treated patients relative to controls (p<0.05). These levels increased after 6 wk of supplementation (p<0.05) and showed no significant differences with those at the end of the study.

Evaluation of oxidative status in iron deficiency anemia through total antioxidant capacity measured using an automated method

“…Oxidant levels and antioxidant enzyme activities such as SOD, GSH-Px and CAT have been evaluated in several studies [8][9][10][11][12][13][14]. Although conflicting results have been reported [8,10], it is generally accepted that oxidative stress is increased through increase in oxidant levels and/or decrease in antioxidant enzyme capacities in IDA.…”

“…Generally, decreased GSH-Px activity along with normal or increased SOD and CAT activity are the main findings in IDA [10,11]. However, in some studies, normal GSH-Px activity along with decreased SOD and CAT activity or decrease in all of these activities was also reported [12,13].…”

“…Thus, measurement of individual antioxidants may not accurately reflect the true antioxidant status of the organism. In this regard, measurement of TAC should be essential in evaluating the true antioxidant status [12,15,19,20].…”

“…Increased oxidative stress in IDA subjects was reported in several studies, in which the oxidative status was evaluated using measurement of oxidants, individual antioxidants, or both [8][9][10][11][12][13][14]. In these studies, it has been reported that oxidants were increased and antioxidants were decreased, and as a result, the oxidative/anti-oxidative balance shifted toward the oxidative side in patients with IDA.…”

Oxidative Stress, Hepcidin and Nesfatin-I Status in Childhood Iron and Vitamin B12 Deficiency Anemias

“…In agreement with our study, their study in patients with iron deficiency anemia also reported an increase in the levels of oxidant products, such as malondialdehyde and a decrease in the levels of the antioxidant enzymes, such as glutathione peroxidase. 16 In anemias, due to Thalassemia and G6PD enzyme deficiency, it has been shown that oxidative stress increases and antioxidant capacity is reduced. 17 In contrast, Acharya et al reported an increase in the levels of antioxidant enzymes in patients with iron deficiency anemia, and they suggested that this was due to overcompensation against the induced oxidative stress.…”