Effect of Isatis spp. Extraction on the Growth of Aspergillus niger and Candida albicans

Abstract

In this study, The crude extraction of Isatis spp. (I.tinctoria, I.busichaina and I. lusitanica) were investigated for their antifungal activity. Each of methanol, ethanol, and H2O was used as a solvent. Due to find the most effective part of each species, the plant parts were used separately. The aim of this research was to determine the effect of the natural product on two common pathogenic fungi Aspergillus niger & Candida albicans. The results show that most of the used plants have a significant effect on both used fungal species. Extracted each of flower, I. tinctoria was shown the best results comparing to the other used species. Extracted leaf and flower of I. tinctoria by methanol was show the best result on Aspergillus niger & Candida albicans. The growth zone was around 90mm for control and 61mm at 75% concentration of methanolic and ethanolic extraction. The flower was for followed by the stem. Depend on the results, the methanol was shown the highest number, then ethanol and the lowest inhibition zone area was for H2O extraction. 

Gastro-protective and therapeutic effect of Punica granatum against stomach ulcer caused by Helicobacter Pylori

“…The samples were air-dried indoors to protect them from direct sunlight. The method described by (12,13) was used for the aqueous extract preparation of the finely powdered samples. Hot distilled water (85ºC) was added and left overnight to prepare a high dose (25g/250ml), and a low dose (25g/500ml), then the extract was filtered by filter paper Whatman No.1.…”