Effect of Isomeres of Triiodothyronine on Erythrokinetics.

Donati, Robert M.; Warnecke, M. A.; Gallagher, Neil I.

doi:10.3181/00379727-122-31359

Cited by 5 publications

(3 citation statements)

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“…Our results are consistent with the in vivo studies of Malgor et al(1975) who showed erythropoietic stimulation by T, and T, that was not dependent on the stimulation of erythropoietin production. Similarly, the dextrorotary isomer of T, has been demonstrated to have erythropoietic activity in vivo (Bozzini et al, 1969;Donati et al, 1966). There is also other evidence for a noncalorigenic effect of thyroxine on erythropoiesis (Meineke & Crafts, 1964).…”

Section: Discussionmentioning

confidence: 98%

Thyroid Hormones Stimulate Erythropoiesis in Vitro

et al. 1977

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Thyroid hormones have important effects on erythropoiesis in man and animals. We performed in vitro culture studies with murine and human bone marrow in order to define the interaction of these hormones with erythroid and granulocyte-monocyte progenitor cells. The methylcellulose clonogenic assay was used with the appropriate addition of erythropoietin or colony-stimulating activity. L-thyroxine, D-thyroxine and L-triiodothyronine potentiated erythropoientin-stimulated erythroid colony formation in concentrations of 50-100 ng/ml. These hormones had no effect on granulocyte-monocyte colony formation at concentrations up to 500 ng/ml. Testing of various thyroid analogues showed no clear correlation between potentiation of erythropoiesis and known calorigenic potency. Reverse triiodothyronine also had potentiating activity in this system. The active thyroid hormones stimulated erythroid colony formation at several concentrations of erythropoietin but could not substitute for erythropoietin. These data suggest that thyroid hormones have a direct effect on erythroid precursor proliferative capacity, a finding which may have relevance to the mechanism of erythropoietic dysfunction in human thyroid disease.

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Section: Discussionmentioning

confidence: 98%

Thyroid Hormones Stimulate Erythropoiesis in Vitro

et al. 1977

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“…However, p58 could also have additional functions. Thyroid hormones were found to increase erythrocyte production in animals (Donati et al, 1966) and cause erythroid proliferation (Popviz et al, 1977). Cytosolic T3 binding protein was shown to be present in erythrocytes (Defer et al, 1975;Yoshida & Davis, 1977;Michelot et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

A cytoplasmic thyroid hormone binding protein: characterization using monoclonal antibodies

Obata¹,

Fukuda²,

Liang³

et al. 1989

Biochemistry

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We have previously purified a cellular thyroid hormone binding protein (p58) from a human carcinoma cell line [Kitagawa, S., Obata, T., Hasumura, S., Pastan, I., & Cheng, S.-y. (1987) J. Biol. Chem. 262, 3903-3908]. In the present study, the binding characteristics, the molecular properties, and subcellular localization of p58 were further characterized. Binding of the purified p58 to thyroid hormones was examined. Analysis of binding data indicates that p58 binds to 3,3',5-triiodo-L-thyronine (T3) with a Kd of 24.3 +/- 0.3 nM and n = 0.71. p58 binds to L-thyroxine similarly as to T3. However, D-T3 and reverse-T3 bind to p58 with an affinity 4- and 20-fold less than that of T3, respectively. By use of the purified p58 as an immunogen, two hybridomas, J11 and J12, secreting monoclonal antibodies to p58 were isolated; both antibodies belong to the IgG1K subclass. J12 recognizes p58 from human, monkey, dog, hamster, and rat, but not mouse. J11 exhibits a similar species specificity except that it does not react with p58 from hamster. With these antibodies, p58 was found to be not posttranslationally modified by glycosylation, sulfation, or phosphorylation. It has a cellular degradation rate t1/2 congruent to 2.1 h. Immunocytochemical studies indicate that p58 is located in the nonmembranous cytoplasm (cytosol). These results are consistent with subcellular fractionation studies which show that greater than 95% of J11 and J12 reactivity and T3 binding activity can be found in the 110,000g supernatant.

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“…In contrast, most hyperthyroid patients have an elevated erythrocyte mass (2). Thyroid hormone increases erythrocyte production in euthyroid experimental animals as well (3). This enhanced erythropoiesis has been attributed to a direct hormonal effect on the erythroid marrow (4,5) and to increased erythropoietin (ESF)l secretion (6,7).…”

Section: Introductionmentioning

confidence: 99%

The Influence of Thyroid Hormones on In Vitro Erythropoiesis

Popovic¹,

Brown²,

Adamson³

1977

J. Clin. Invest.

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A B S T R A C T The erythropoietic effect of various thyroid hormones has been studied using erythroid colony formation by canine marrow cells. Although erythropoietin was required for colony growth, physiologic levels ofthyroid hormones significantly enhanced colony numbers. The order of potency of the thyroid compounds in their in vitro erythropoietic effect parallels their known calorigenic potency in vivo, suggesting that the in vitro effect is physiologically relevant. A series ofstudies linked the mechanism ofthyroid action to adrenergic receptors on responsive cells. Propranolol, a global ,8-blocker, inhibited thyroid hormoneresponsive erythroid colonies. When adrenergic antagonists having different blocking characteristics were added to culture, the thyroid hormone effect was blocked by those compounds having ,82-subspecificity.Velocity sedimentation analysis showed that the peak of colony-forming cells which respond to thyroid hormone and the adrenergic agonist, isoproterenol, sedimented at an identical rate (7.54 mm/h), which is slower than the major peak ofcolony-forming cells responding to erythropoietin alone (8.62 mm/h). These results demonstrate thyroid hormonal enhancement of in vitro erythroid colony growth which appears mediated by a receptor with (82-adrenergic properties.

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Effect of Isomeres of Triiodothyronine on Erythrokinetics.

Cited by 5 publications

References 0 publications

Thyroid Hormones Stimulate Erythropoiesis in Vitro

Thyroid Hormones Stimulate Erythropoiesis in Vitro

A cytoplasmic thyroid hormone binding protein: characterization using monoclonal antibodies

The Influence of Thyroid Hormones on In Vitro Erythropoiesis

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