Effect of isothermal radiofrequency radiation on cytolytic T lymphocytes

Abstract

Previous in vitro studies provide evidence that RF electromagnetic radiation modulates proliferation of human glioma, lymphocytes, and other cell types. The mechanism of RF radiation cell proliferation modulation, as well as mechanisms for effects on other cell physiologic endpoints, are not well understood. To obtain insight regarding interaction mechanisms, we investigated effects of RF radiation exposure on interleukin 2 (IL-2) -dependent proliferation of cytolytic T lymphocytes (CTLL-2). After exposure to RF radiation in the presence or absence of IL-2 cells were cultured at various physiological concentrations of IL-2. Treatment effects on CTLL-2 proliferation were determined by tritiated thymidine incorporation immediately or 24 h after exposure. Exposure to 2450 MHz RIF radiation at specific absorption rates (SARs) of greater than 25 W/kg (induced E-field strength 98.4 V/m) induced a consistent, statistically significant reduction in CTLL-2 proliferation, especially at low IL-2 concentrations. At lower SARs, 2450 MHz exposure increased CTLL-2 proliferation immediately after exposure but reduced 24 h postexposure proliferation. RF radiation effects depended on the mitotic state of the cells at the time of exposure. Comparison of the effects of temperature elevation and RF radiation indicated significant qualitative and quantitative differences.

900 MHz pulse-modulated radiofrequency radiation induces oxidative stress on heart, lung, testis and liver tissue

“…Decreased sperm count and capacity of quails were reported by McRee et al (1983) as a result of MW exposure. Cleary et al (1996) showed that RF radiation may reduce fertilizing capacity of spermatozoa. Agarwal et al (2009) suggested that RF EMRinduced oxidative stress in semen may be responsible for decreased sperm motility and viability.…”

“…Moreover, some authors suggested that mechanism of RF/MW (microwave) radiation damage may be attributable to the induction of oxidative stress leading to free radical generation and radical scavengers' alterations in biological tissues or cells as a result of the interaction of the tissues or cells with RF/MW fields (Cleary et al 1996;Diem et al 2005). According to the current assumption electromagnetic (EM) fields may initiate oxygen-and nitrogen-free radical intermediates and several types of tissue injury may be associated with oxidative stress process induced by these fields.…”

Radiofrequency exposure and mammalian cell toxicity, genotoxicity, and transformation

“…Cleary et al [1996] exposed CTLL-2 cytolytic T lymphocytes to a 2450 MHz signal for 2 h, at SARs ranging up to 50 W/kg. The temperature was said to be maintained at 37 8C.…”

Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes

“…When rats were exposed to RF/ MW radiation to reproduce normal human exposure, the life span and tumorigenicity of rats were unchanged [1,21] but evidence of oxidative damage was found in brain tissues [19,29]. RF/MW radiation induced damage can lead to death in single cell organisms [3], inhibit cell proliferation [9], cause DNA damage [11,32], and alter gene expression in different cell types including brain cells as measured by gene microarrays [6,14,15]. Although the methods and target cells differ among the report cited above, the existing literature taken as a whole suggests that the expression of specific genes and proteins in cultured cells and intact animals can be affected by RF/MW radiation exposure [10].…”