Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

Poppi, Larissa Batista; Rivaldi, Javier D.; Coutinho, Thais S.; Astolfi-Ferreira, Claudete S.; Ferreira, Antônio José Piantino; Mancilha, Ismael Maciel de

doi:10.1590/s0100-736x2015000400007

Cited by 37 publications

(29 citation statements)

References 34 publications

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“…Keterangan: n = jumlah ulangan ; t= jumlah kelompok perlakuan dan kelompok kontrol (6 Poppi et al, 2015).…”

Section: Metodeunclassified

“…Filtrat yang diperoleh disterilkan menggunakan filter milipore 0,2 μm (Poppi et al, 2015dan Fauziah dkk., 2014 (Dahlan, 2011).…”

Section: Metodeunclassified

“…Pada uji Post Hoc (Mann-Whitney), didapatkan bahwa pada kelompok konsentrasi 25% dan 50% tidak terdapat perbedaan Penelitian ini sesuai dengan penelitian Poppi et al (2015) yang menguji efektivitas antibakteri filtrat L. plantarum terhadap E. coli, didapatkan hasil bahwa asam laktat dan asam asetat yang terdapat pada filtrat dapat menghambat pertumbuhan E. coli dengan diameter zona hambat sebesar 18 mm. Pada penelitian Maunatin dan Khanifa (2012) dengan menggunakan metode sumuran, L. plantarum dapat menghambat S. aureus dan S. typhi dengan diameter zona hambat masing-masing sebesar 13,3 mm dan 9,3 mm.…”

Section: Pembahasanunclassified

“…Kadar asam laktat dan asam asetat yang dihasilkan L. plantarum B122 pada penelitian ini lebih besar dari L. plantarum isolat 22c yang hanya menghasilkan asam laktat dan asam asetat sebesar 18,5g/L dan 4,2g/L (Poppi et al, 2015). Hal ini karena metabolit yang dihasilkan oleh bakteri asam laktat dipengaruhi oleh kondisi fermentasi dan strain bakteri asam laktat tersebut (Yuliani, 2005).…”

Section: Pembahasanunclassified

“…Menurut penelitian Poppi et al (2015), L. plantarum menghasilkan asam laktat dan asam asetat yang dapat menghambat pertumbuhan E. coli. Asam laktat yang dihasilkan L. plantarum mampu menurunkan pH menjadi rendah sehingga bakteri patogen akan sulit bertahan hidup (Tambekar and Bhutada, 2010).…”

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Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro

2017

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Shigella dysenteriae is pathogenic bacteria causes dysentery diarrhea, a diarrhea with mucus and blood in the feces. Currently, drugs or foods containing Lactobacillus have been circulated in the markets and used by people. This research aims to know the in vitro inhibition test of L. plantarum metabolite substances filtrate against growth of S. dysenteriae. This research used experimental design with L. plantarum metabolite substances filtrate as the samples. Antibacterial test method which was used was the cup-plate technique by looking at clear zone of bacteria's growth around cup-plate and used Mueller Hinton Agar media. The results showed clear zones on filtrate with concentrations 25%, 50%, 75%, and 100% with average inhibition zones 0,284 cm; 0,332 cm; 0,406 cm; and 0,619 cm. Kruskal-Wallis test results indicated there were significant differences of each filtrate concentration against S. dysenteriae (p<0,05). Post Hoc test showed no significant difference between concentrations 25% and 50% (p>0,05). This suggested lactic acid, acetic acid, and bacteriosin presented in L. plantarum metabolite have antibacterial characteristic. Keywords: antibacterial, Lactobacillus plantarum, Shigella dysenteriae INTISARIShigella dysenteriae merupakan bakteri patogen penyebab diare disentri, yaitu kondisi diare disertai lendir dan darah dalam feses. Saat ini, sediaan obat atau makanan yang mengandung Lactobacillus telah beredar di pasaran dan dimanfaatkan oleh masyarakat. Tujuan penelitian ini adalah untuk mengetahui daya hambat filtrat zat metabolit L. plantarum terhadap pertumbuhan S. dysenteriae secara in vitro. Penelitian ini menggunakan desain eksperimental dengan sampel filtrat zat metabolit L. plantarum. Metode uji antibakteri yang digunakan adalah metode sumuran dengan melihat zona hambat pertumbuhan bakteri di sekitar sumuran. Hasil penelitian ini memperlihatkan adanya zona bening pada konsentrasi filtrat 25%, 50%, 75%, dan 100% dari pertumbuhan S. dysenteriae dengan rata-rata zona hambat 2,84 mm; 3,32 mm; 4,06 mm; dan 6,19 mm. Hasil uji Kruskal-Wallis menunjukkan perbedaan signifikan masing-masing konsentrasi filtrat terhadap S. dysenteriae (p<0,05). Pada uji Post Hoc, kelompok konsentrasi 25% dan 50% menunjukkan tidak terdapat perbedaan signifikan (p>0,05). Penelitian ini menunjukkan bahwa asam laktat, asam asetat, dan bakteriosin yang terdapat pada metabolit L. plantarum dapat bersifat sebagai antibakteri.Kata kunci: antibakteri, Lactobacillus plantarum, Shigella dysenteriae PENDAHULUANDiare merupakan masalah kesehatan dunia karena morbiditas (angka kesakitan) dan mortalitasnya (angka kematian) yang tinggi serta merupakan salah satu dari tiga penyakit utama selain pneumonia dan malaria, yang menyebabkan kematian pada anak-anak (WHO, 2012). Diare adalah suatu kondisi buang air besar dengan konsistensi lembek atau cair, bahkan dapat berupa air

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“…Keterangan: n = jumlah ulangan ; t= jumlah kelompok perlakuan dan kelompok kontrol (6 Poppi et al, 2015).…”

Section: Metodeunclassified

“…Filtrat yang diperoleh disterilkan menggunakan filter milipore 0,2 μm (Poppi et al, 2015dan Fauziah dkk., 2014 (Dahlan, 2011).…”

Section: Metodeunclassified

Section: Pembahasanunclassified

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Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro

2017

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Cell-Free Lactobacillus sp Supernatants Modulate Staphylococcus aureus Growth, Adhesion and Invasion to Human Osteoblast (HOB) Cells

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Evaluation of Lactobacilli for Antagonistic Activity Against the Growth, Adhesion and Invasion of Klebsiella pneumoniae and Gardnerella vaginalis

2018

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Urinary tract infections are the most common infectious diseases in babies and the elderly and are often acquired as nosocomial infections. The purpose of the present study was to identify strains of lactic acid bacteria (LAB) which could be used as alternatives to antibiotics for the treatment of urinary tract infections because of their ability to inhibit urinary tract pathogens (Klebsiella pneumoniae BCRC 10694 and Gardnerella vaginalis BCRC 17040). We screened 370 LAB strains using spent culture supernatants by inhibition zone assay to assess their antimicrobial effects. We studied the effect of heat, pH and enzyme treatment on the inhibitory activity of LAB strain supernatants. Anti-growth activity against urinary tract pathogens was evaluated by co-culture inhibition assay using seven LAB strains. Anti-adhesion and anti-invasion activities against urinary tract pathogens were evaluated by SV-HUC-1 uroepithelium cell culture. The results showed that the supernatants had good heat stability. However, antibacterial activity disappeared entirely at pH 7.0. After enzyme treatments, the supernatants showed first-or second-order inhibitory effects on K. pneumonia BCRC 10694. The survival rate of urinary tract pathogens was 0-10.65% and pH of the culture medium decreased after co-culture with LAB strains for 4 h. In a competition assay, PM2 and RY2 inhibited urinary tract pathogens. PM68, PM78, PM201, PM206 and PM229 inhibited the invasion of SV-HUC-1 cells by G. vaginalis BCRC17040. In conclusion, PM78, PM229 and RY2 showed the strongest inhibitory activity against urinary tract pathogens and could be suitable for use in the treatment of urinary tract infections.

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Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

Cited by 37 publications

References 34 publications

Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro

Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro

Cell-Free Lactobacillus sp Supernatants Modulate Staphylococcus aureus Growth, Adhesion and Invasion to Human Osteoblast (HOB) Cells

Evaluation of Lactobacilli for Antagonistic Activity Against the Growth, Adhesion and Invasion of Klebsiella pneumoniae and Gardnerella vaginalis

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