Effect of lactoferrin on the production of tumor necrosis factor-? and nitric oxide

Choe, Yoon-Hyuk; Lee, Soo-Won

doi:10.1002/(sici)1097-4644(20000101)76:1<30::aid-jcb4>3.0.co;2-u

Cited by 46 publications

(26 citation statements)

References 21 publications

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“…Although Lf was first described as an ironbinding molecule with antibiotic properties [3], it is now known to be a multifunctional protein [4,5] with anticancer and anti-inflammatory activities [6][7][8][9]. The immunomodulatory activity of Lf affects the production of cytokines such as TNF-a, IL-1b, IL-6, IL-8, NO, and GMCFS [10][11][12][13]. In addition to its effects on the immune system, Lf inhibits tumor metastasis by inhibiting tumor growth and the number of tumor-induced blood vessels [14], suggesting a possible anti-angiogenic activity.…”

Section: Introductionmentioning

confidence: 70%

Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

et al. 2010

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Section: Introductionmentioning

confidence: 70%

Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

et al. 2010

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“…This stimulatory activity may be attributed to Lactoferrin forming a complex with LPS to facilitate macrophage activation through CD14 (LPS co-stimulatory molecule) or the TLR-4 (LPS receptor) [36]. Previous studies on LPS activated macrophages and models of sepsis also reported this inhibitory effect of Lactoferrin to moderate other proinflammatory mediators [9,37]. Certainly, the ability of Lactoferrin to inhibit LPS-induced production of TNF-α and IL-6 is present when Lactoferrin is administered prophylactically (and even therapeutically) [29], or even when given therapeutically.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin modulation of IL-12 and IL-10 response from activated murine leukocytes

Hwang¹,

Wilk²,

Bangale³

et al. 2007

Med Microbiol Immunol

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Lactoferrin possesses a wide range of immunomodulatory activities, including promotion of the delayed type hypersensitivity response (DTH) towards BCG (Bacillus Calmette Guerin) antigens. Addition of Lactoferrin as an adjuvant to the BCG vaccine was previously demonstrated to augment protection against subsequent mycobacterial challenge, with concomitant development of a strong T cell helper type 1 (T H 1) immunity. Because generation of T H 1 immunity is in large part dependent on the balance of monocytic pro-and anti-inflammatory cytokines, the effect of Lactoferrin on leukocytes was investigated. Lactoferrin enhanced proinflammatory responses in a dose-dependant manner from splenocyte and adherent (F4/80 + ) splenocyte populations, bone marrow derived monocytes (BMM), and J774A.1 cultured cells. In all scenarios tested, Lactoferrin induced a strong increase in the ratio of IL-12:IL-10 production from LPS stimulated cells. Examination of Lactoferrin effects on BCG infected J774A.1 cells and on BMM revealed similar immunomodulatory effects, with particularly strong increase in IL-12 production. Furthermore, immunization of mice with BCG admixed with Lactoferrin led to increased generation of CD4+ cells expressing IFN-γ upon restimulation with BCG antigens. These results provide molecular evidence to support the role of Lactoferrin as an adjuvant candidate to augment development of DTH response to vaccine antigens.

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“…BoSA cells: It has been reported that the production of superoxide by leukocytes is stimulated by bovine Lf [6,7]. O 2 -production was 3.564 ± 0.062 nmol/10 6 cells/60 min on stimulation with low Con A affinity Lf.…”

Section: Physiological Effect On Peripheral Blood Lymphocytes Andmentioning

confidence: 94%

Small Molecule Lactoferrin with an Inflammatory Effect But No Apparent Antibacterial Activity in Mastitic Mammary Gland Secretion

Komine¹,

Komine²,

Kuroishi³

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. We have identified various lactoferrin (Lf) molecules in mastitic mammary gland secretions (MGSs), and these Lf molecules were examined for their physiological function in MG. These Lf molecules were isolated by Con A affinity chromatography, and then analyzed by various electrophoresis methods and N-terminal amino acid sequencing. The low Con A affinity Lf was found to have low molecular peptides as compared with the 86 kDa of the high Con A affinity Lf, which is usually detected in healthy MGSs. The Nterminal amino acid sequence of each of the small molecular Lfs were confirmed as fragments of 86 kDa Lf. This low Con A affinity Lf stimulated spleen adherent cells to produce more O 2 -than 86 kDa Lf. Furthermore, the low Con A affinity Lf showed low antibacterial activity against E. coli and S. aureus, and had decreased iron-binding capacity in comparison with 86 kDa Lf. Moreover, the 86 kDa Lf could stimulate bovine T cells or macrophages to produce IFN-γ, IL-4, IL-6, and IL-1α. However low Con A affinity Lf induced the production of TNFα, but not physiological T cell or macrophage cytokines. It was also found that when the healthy MGs of dry cows were injected with the low Con A affinity Lf, there was an increase in polymorphonuclear cells together with TNFα, MCP-1, and IL-8 production. These results suggested that low Con A affinity Lf in mastitic MGSs differed from 86 kDa Lf in physiological characteristics, and, that it induced an inflammatory reaction in MGs. KEY WORDS: concanavalin A, lactoferrin, mammary gland secretion, mastitis.

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Effect of lactoferrin on the production of tumor necrosis factor-? and nitric oxide

Cited by 46 publications

References 21 publications

Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

Lactoferrin modulation of IL-12 and IL-10 response from activated murine leukocytes

Small Molecule Lactoferrin with an Inflammatory Effect But No Apparent Antibacterial Activity in Mastitic Mammary Gland Secretion

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