Effect of Langmuir monolayer of bovine serum albumin protein on the morphology of calcium carbonate

Abstract

a b s t r a c t Keywords:Biomineralization Crystal morphology Langmuir monolayer Bovine serum albumin Calcite Bovine serum albumin (BSA) Langmuir monolayer, as a model of biomineralization-associated proteins, was used to study its effect on regulated biomineralization of calcium carbonate. The effects of the BSA Langmuir monolayer and the concentration of the subphase solution on the nucleation and growth processes and morphology of the calcium carbonate crystal were investigated. The morphology and polymorphic phase of the resulting calcium carbonate crystals were characterized by scanning electron microscopy (SEM) and Xray diffraction analysis (XRD). Moreover, the interaction mechanisms of the subphase solution with the BSA Langmuir monolayer were discussed. It was found that BSA Langmuir monolayer could be used as a template to successfully manipulate the polymorphic phase and crystal morphology of calcium carbonate and had obvious influence on the oriented crystallization and growth. The final morphology or aggregation mode of the calcite crystal was closely dependent on the concentration of calcium bicarbonate solution. It is expected that this research would help to better understand the mechanism of biomineralization by revealing the interactions between protein matrices and crystallization of calcium carbonate crystal.

Protein-Mediated Precipitation of Calcium Carbonate

“…At a neutral pH, this biomacromolecule possesses a large number of negatively-charged carboxyl groups [26]. According to literature data, the protein macromolecules formed a framework at the solution surface, and the calcium ions were bound to these chains via the carboxyl groups [26,30,31]. The size of particles synthesized in the presence of ovalbumin is not dependent on the duration of the process.…”

“…In addition, during the initial stage of shell formation, ovalbumin, ovotransferrin, and lysozyme are present in the environment where the calcification of the shell occurs [24]. In recent years, based on fact that the biomineralized materials often contain proteins that are rich in glutamic or aspartic acid residues, various single proteins have been developed to control the mineralization process of calcium carbonate in a solution [23,24,25,26]. Due to the high complexity of the constituents of the organic matrix, the eggshell formation mechanism and the contribution of each constituent remain poorly understood, and more investigation is required using model systems where mixtures of macromolecules are present.…”

Laser induced effects on ZnO targets upon ablation at 266 and 308nm wavelengths

“…16 A slopping background is also observed in these Raman spectra that can be attributed to visible luminescence produced in the postablated areas. 14 The above results show that oxygen vacancies are developed on the ZnO target surface by 308 nm laser irradiation. Moreover, the monotonic development of the characteristic Raman spectra indicates that the oxygen vacancy concentration increases monotonically toward the crater center in the same way that the laser fluence distribution on the crater; the latter corresponds to the nearly Gaussian profile of the energy distribution across the transversal section of the focused laser beam.…”

“…In most of the Raman spectra of nanoparticles, the first-order Raman peak redshifts and broadens toward the low-wave-number side, as the nanoparticle size decrease; 13,14 however, unexpected upward shifts have also been observed in nanoparticles embedded in a glass matrix. 14 The above suggests that the structural changes observed in the Raman spectra might be due to the formation of nanostructured centers in the targets, above certain laser fluence.…”

Calcium carbonate microspheres as carriers for the anticancer drug camptothecin

“…Bovine serum albumin (BSA), a major protein component of bovine plasma (5 g/100 mL), has been utilized as a carrier protein in many vaccines and medicines and as a stabilizing agent in enzymatic reactions [43]. Recently, BSA has been used as a template to induce the formation of calcium carbonate [44] and stabilize vaterite [45]. Since both calcium carbonate and BSA have good biocompatibility and biodegradable properties, BSA templated CCMS will have potential applications in the pharmaceutical and biomedical areas.…”