Effect of lantibiotic gallidermin against biogenic amine-producing faecal staphylococci from ostriches and pheasants

Abstract

In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococci from ostriches and pheasants and their sensitivity to lantibiotic bacteriocin gallidermin. Gallidermin belongs in a group of polycyclic proteinaceous antimicrobial substances. Thirty-six faecal staphylococci (24 strains from 140 ostriches, 12 from 60 pheasants) comprising different species were tested. Staphylococci from ostriches and pheasants did not produce tryptamine-TRYP, putrescine-PUT, cadaverine-CAD or histamine-HIS. Production of tyramine-TYM, phenylethylamine-PEA was high or very high (100-1000 mg/L). Production of spermine-SPM and spermidine-SPD by staphylococci was very low or low although in the case of staphylococci from pheasants medium production of SPM was found. Because of the risk posed by BAs for consumers, the control of BA-producing bacteria is important from the points of view not only of safety assessment of food-producing animals but also of human health safety. The sensitivity to gallidermin in biogenic amine-producing staphylococci from ostriches and pheasants detected here is the most promising indication for further application of gallidermin for veterinary purposes. The novelty of our study lies in testing the ability of faecal staphylococci from ostriches and pheasants to produce BAs and in their treatment with gallidermin which has so far not been tested in this way.

Slovak raw goat milk as a source of variable, biofilm‐forming staphylococci, and their susceptibility to lantibiotic bacteriocins

“…These residues can negatively influence technological procedures during goat milk product processing. 6 However, antimicrobials such as bacteriocins can inhibit spoilage, antibiotic-resistant, and/or biofilm-forming bacteria as also previously reported by Lauková et al 7,8 Gallidermin is a polypeptide bacteriocin, which was first characterized in the strain Staphylococcus gallinarum TU 3928. It contains amino acid residues lanthionine, β-methyllanthionine, or α,β-didehydroamino acids.…”

“…Even in vivo reduction of staphylococci by nisin and gallidermin has been reported for instance in broiler rabbits 21,31 and also in vitro. 7 Gallidermin inhibited growth of biogenic amine-producing fecal staphylococci from ostriches and pheasants with activity up to 25,600 AU/ml. Nisin did not influence biochemical parameters in broiler rabbits; however, increase in body weight was noted (about 9.4%), significant increase in phagocytic activity (p < 0.001) and significant decrease in staphylococci (p < 0.01).…”

Susceptibility to Bacteriocins in Biofilm-Forming, Variable Staphylococci Isolated from Local Slovak Ewes’ Milk Lump Cheeses

“…The mode of action of gallidermin is similar, as it integrates into the plasma membrane, forming pores and inhibiting cell membrane synthesis [ 38 ]. Similarly, as in this study, variable fecal staphylococcal strains of different species isolated from ostriches and pheasants, and producing biogenic amines, were inhibited by gallidermin, reaching inhibition zones in the range from 3200 up to 25,600 AU/mL [ 39 ]. All results from previous studies and the present one underline the effective use of enterocins and lantibiotic bacteriocins against contaminating bacteria, representing an opportunity which could be used in further practice.…”