Effect of laser therapy on immune cells infiltrate after excisional wounds in diabetic rats

Abstract

The present findings demonstrate that laser therapy can alter the composition of inflammatory infiltrate in diabetic wounds, leading to a more balanced response transiting from a rapid neutrophil infiltration through to M2 macrophage polarization, especially with a single application of 4 J/cm(2) in the immediate postoperative period.

Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium

“…Furthermore, the PBMT group had more M2 macrophages compared with that of the control group on days 8 and 10. 30 Consistently, in the current study, all treatment groups presented decreased neutrophil and macrophage counts and increased number of fibroblasts and angiogenesis on days, 4, 7, and 15. Here, the increased fibroblasts and angiogenesis of the treatment groups in our study support the anti-inflammatory and repairing roles of M2 macrophages in an infected wound model, in T1DM rats.…”

Assessment of wound area reduction on chronic wounds in dogs with photobiomodulation therapy: A randomized controlled clinical trial

“…In clinical case reports, human venous ulceration wounds treated with 830 nm PBMT with a dose of 9 J/cm 2 [34] and chronic dog wounds treated with the use of a single 630 nm wavelength PMBT with a fluence of 5 J/cm 2 [28] exhibited more rapid wound area reduction. This potentially confirms in vitro studies showing that PBMT improved biological immune response by increasing the migration of primary cytokines (interleukin-1β [IL-1β], tumor necrosis factor-α, IL-6, and MCP-1) [35], neutrophil and macrophage infiltration [36], angiogenesis, fibroblast and collagen formation, reepithelialization, and wound tensile strength [37][38][39]. At present, evidence of the use of simultaneous SPMW-PBMT on wound healing in dogs is limited.…”

Efficacy of Biophysical Energies on Healing of Diabetic Skin Wounds in Cell Studies and Animal Experimental Models: A Systematic Review

“…All trials provide baseline clinical characteristics including gender, age or weight of the subjects. In addition, all expected outcomes are reported [19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,46,47,48,49,55,56,57,58,59,60,61,62,63,66,67,68,69,70,71,74,76,77,78,79,80,82,83,84,85,90,91,92,95,96,97,99,100]. Only one trial provides an adequate report on allocation concealment [24].…”

“…Five trials report the non-random approach when placing the animals within the facility [62,63,66,71,92]. None of the trials provide information about investigator blinding, but twenty trials report outcome assessor blinding [21,24,25,42,43,44,60,62,67,68,70,78,79,84,86,87,92,95,99,100]. Three trials report random outcome assessment, although no detailed method of randomization is provided [23,86,87].…”

“…Most trials report positive results, except Jahangiri Noudeh et al who found no statistical significance by repeated measurements throughout the entire study period when a combined 670 nm and 810 nm laser was applied to wound areas [66]. Histological analysis revealed that there was an increase in macrophages [61,95,99], fibroblasts [47,53,63,67,68,81,84,99,100], neutrophils [95], T lymphocyte [95], collagen deposition [37,40,70,77,82,85,99,100], nitrite [100] and nitric oxide level [65], catalase activity [100], thiobarbituric acid reactive substances [100] and vascularization [44,68,70,99] after irradiation. Chung et al adopted a splinted diabetic wound model to minimize mouse skin contraction during wound healing [62].…”