Effect of lead acetate alone and in combination with whole milk (Star ship®) on body growth and liver functions in an experimentally induced lead toxicity in rat

Gani, Mohammad Usman; Siddiqui, Saiful Islam; Rashid, Harun Or; Islam, Kamrul; Moonmoon, Sharifunnessa; Ahmed, Shaifuddin; Mostofa, M

doi:10.3329/ajmbr.v2i2.29009

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…These results are consistent with Elgawish and Abdelrazek (2014), who reported a significant reduction in testicular weight in lead‐treated rats. Gani et al., (2016) reported a significant reduction in rats’ body weights after treatment with lead acetate. This was explained by the interruption of absorption and metabolism of food and nutrients essential for health.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

Abdel‐Emam

Ahmed

2021

Veterinary Medicine & Sci

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Lead toxicity is one of the causative agents of male infertility that raised concern from environmental contamination worldwide. L-carnitine, a biologically active amino acid, present in high concentration in the reproductive organs such as the epididymis, is involved in sperm maturation. The possible protective effect of L-carnitine in experimentally lead-induced male reproductive toxicity in rats was evaluated in this study.Thirty adult male Wistar rats were divided into three groups. Group 1: the negative control group was treated with normal saline; Group 2: exposed to 50 mg/kg lead acetate (2% solution in saline); and Group 3: treated with lead acetate 50 mg/kg (2% solution in saline) + L-carnitine 100 mg/kg. At the end of the experimental period, body and testicular weights were determined, blood samples were withdrawn for hormonal assays of FSH, LH and testosterone. Sperm parameters as sperm count, morphology, viability and motility were measured. Testicular tissue homogenates were prepared for enzymatic assays and for measuring oxidative stress parameters.Lead significantly increased both oxidative stress and the concentration of lactate dehydrogenase-C in the testicular tissues with a decrease in sperm count, motility and viability. Lead acetate treatment, induced alteration in sperms with normal morphology together with reductions in the serum FSH, LH, testosterone, body and testicular weights. The concentration of 17β-hydroxysteroid dehydrogenase was significantly reduced. Co-administration of L-carnitine significantly reduced testicular oxidative stress, improved sperm parameters, elevated serum FSH, LH and testosterone with an insignificant reduction in the testicular weight. The concentrations of 17β-hydroxysteroid dehydrogenase and lactate dehydrogenase-C were significantly improved by L-carnitine. The overall results indicate that L-carnitine is expected to improve the lead acetate-induced male reproductive toxicity.

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Section: Discussionmentioning

confidence: 99%

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

Abdel‐Emam

Ahmed

2021

Veterinary Medicine & Sci

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“…Lead is a ubiquitous environmental toxin that has been detected in almost all phases of biological systems [1]. Lead results from the burning vehicle fuel, settles into the soil, and is absorbed by the plant, so food can be the source of lead exposure.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Nephroprotective Effects of Okra Pods Extract (Abelmoschus esculentus L.) against Lead Acetate-Induced Toxicity in Mice

et al. 2020

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In this study, we determine the curative effects of okra pods (Abelmoschus esculentus L.) extract against lead acetate toxicity in mice kidney. n-Hexane, ethyl acetate, and methanol solvent were used for extracting okra pods. The role of the extract as an antioxidant was tested by DPPH and FRAP methods. The methanol extract was used for experiments in animals. A total of 30 male BALB/c mice were randomly divided into six equal groups: normal control, negative control (lead-induced), and treatment groups (lead-induced for 28 days and administration of methanol extract at doses of 50, 100, 200, and 400 mg/kg BW for the 28 days). The following were analyzed in all groups: activity of the antioxidant enzymes, namely, superoxide dismutase (SOD) and catalase (CAT); oxidant level, namely, malondialdehyde (MDA) and nitric oxide (NO); and markers of kidney injury, namely, blood urea nitrogen (BUN) and creatinine (Cre). Kidney histopathology was also evaluated. This study showed that the methanol extract showed the highest antioxidant activity (IC50 is 35.21 µg/mL, and FRAP is 57.58 µM Fe2+/g). The CAT and SOD activities increased significantly in okra-treated groups (P<0.05). The okra administration groups experienced a significant decrease in MDA, NO, BUN, and Cre levels (P<0.05). Thickness of the epithelial proximal tubule, diameter of the proximal tubule, and percentage of necrotic cells in proximal tubule decreased, but the diameter ratio of glomerular Bowman’s capsule in mice treated with okra was optimally improved and repaired like normal control (P<0.05). The results of this study reveal that methanol extract has a very strong antioxidant effect and can reduce the influence of toxicity induced by lead acetate in mice kidney.

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“…Al-Wabel et al (2007), reported that when 50 mg/kg lead acetate was available to rats, the serum concentration of GGT was gradually elevated from 24 IU/L to 38.3 IU/L. Gani et al (2016) revealed the familiar research conclusion when exposed the feed of rats with lead acetate. The authors reported elevated serum values of glutamate pyruvate transaminase and recorded detrimental effects on the hepatic system.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lead Toxicity on Japanese Quail and its Alleviation with Dietary Selenium

Habiullah,

Nawaz Kumbar,

Parveen Samo

et al. 2023

JAHP

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The present study was carried out to investigate the effects of lead toxicity on Japanese quail and its alleviation with dietary selenium. Thirty healthy Japanese quails at the age of about 3 to 4 weeks were divided into six groups viz., A (control group), B (selenium 1.0 mg/L), C (lead acetate 200 mg/L), D (lead acetate 400 mg/L ), E (lead acetate 200 + selenium 1.0 mg/L) and F (lead acetate 400 + selenium 1.0 mg/L). Both selenium and lead acetate were given by adding in the drinking water for 20 days. At the end of experiment, four birds from each group were slaughtered and blood was collected for evaluation of biochemical tests like Gamma-glutamyltransferase (GGT), bilirubin, albumin, uric acid and creatinine. After necropsy examination, the liver and kidney samples were collected for gross and histopathological examination. The mild clinical signs like reduced feed and water intake, and weight loss in group C, D, E and F was noted; whereas diarrhea, muscular tremors, and lethargy were observed in group C and D. Significantly increased (p <0.05) GGT, bilirubin, creatinine and uric acid levels were recorded in C and D (lead toxicity groups), as compared to control group; whereas group E and F (treated with selenium) showed the protective effect by decreasing (p <0.05) GGT, creatinine, bilirubin and uric acid concentration as compare to C and D groups. Gross pathology observed in liver of C, D and F groups revealed inflammation, and discoloration, whereas kidneys were found swollen and congested with distended ureters. Major histopathological changes observed in kidneys of group C, D and F like shrinkage of glomeruli with widened bowman's spaces, necrosis and inflammation. Whereas, liver shows severe dilation of central vein and sinusoids, congestion and necrosis. Group E showed no major changes in liver and kidney while group F has less severity than groups C and D. The present study concluded that selenium has a protective effect against toxicity produced by lead acetate as evident from reduced morbidity, amelioration of liver and kidney function with decrease in serum GGT, creatinine and uric acid and albumin increase; thus selenium might be adapted for strategies against the toxicological effects of lead in avian species.

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Effect of lead acetate alone and in combination with whole milk (Star ship®) on body growth and liver functions in an experimentally induced lead toxicity in rat

Cited by 6 publications

References 16 publications

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

Antioxidant and Nephroprotective Effects of Okra Pods Extract (Abelmoschus esculentus L.) against Lead Acetate-Induced Toxicity in Mice

Effect of Lead Toxicity on Japanese Quail and its Alleviation with Dietary Selenium

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