The Effect of lead acetate alone and in combination with whole milk on body weight gain and some biochemical parameters were carried out on a total of 15 (15 days old) male weaning Long- Evans strain rats. The rats were randomly divided into three equal groups, each consisting of five rats. Rats of group A were kept as control (without giving any treatment), group B received lead acetate alone @ 6mg/ml drinking water and group C received lead acetate @ 6mg/ml plus whole milk (Star ship®) 150 mg/ml drinking water. The result showed that body weight gain of control group per week per rat was found to increase but in treated group B, the body weight gain was found to decrease most significantly (P< 0.01) on day 56 while in group C, body weight was reduced significantly (P< 0.05) on day 56. The reducing body weight gain was less in group C than group B. A most significantly (P< 0.01) increased SGOT and SGPT values were observed in Group B but in group C, those count increased significantly (P< 0.05) on day 56 of experiment. From the study it was concluded that treatment with lead acetate at low doses has adverse effects on body growth and liver functions in experimental animals.Asian J. Med. Biol. Res. June 2016, 2(2): 183-189

Background: Bovine spongiform encephalopathy (BSE) is an emerging zoonotic disease of cattle associated with pathological prion protein (PrP sc ) transmitted via meat and bone meal (MBM). Although Bangladesh did not experience a BSE outbreak but the country could not export animal products to developed countries as has not yet been declared BSE free country by OIE due to lack of scientific risk evaluation for BSE.The objectives were identification of hazard, release and exposure pathways of pathological prion protein through MBM and analysis of risk for the occurrence of BSE in Bangladesh. Methods:The scientific data were reviewed, hazards were scheduled and surveys were conducted on livestock production system, import of MBM and its use to identify the hazards present in Bangladesh context. The analysis was done by the 'OIE Risk Analysis Framework 2006 and European Union (EU) Scientific Steering Committee (SSC) 2003' .From the historical reviews, import of MBM and its use was identified, as external hazards. Results:The analysis revealed that these hazards had negligible or moderate risk for the introduction of infectious PrP sc as Bangladeshi cattle are vegetarian cattle. No milk replacer was used and use of slaughtered waste in the animal feed industry is absent.Unconsumable bones are processed to produce bone chips, fertilizers and bone meal for poultry feeds. Scrapie was never prevalent in Bangladesh. Therefore, risk from the internal challenge was negligible in Bangladesh for the occurrence of classical BSE.These prevented the propagation of BSE infectivity and eliminated BSE infectivity from the system very fast, if that was present. Conclusions:It was concluded that introduction of PrP sc into cattle population of Bangladesh through MBM was very negligible. Therefore, Bangladesh can be considered as BSE negligible risk country.

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Abstract:The effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and effects of sand-charcoal-iron-filter (SCIF)-bed filtrated water along with alum on body weight and some hematological parameters were carried out on a total of 30 (01 month old) adult Newzealand white rabbits. The Rabbits were randomly divided in to 6 equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100 ppm, group C received arsenic trioxide @ 100 ppm plus tannic acid @100 ppm, group D received arsenic trioxide @ 100 ppm plus di-sodium hydrogen phosphate @100 ppm, group E received arsenic trioxide @100 ppm plus alum @100 ppm orally daily for 60 days in all cases and group F received alum @100 ppm in SCIF-bed filtrated water orally daily for 60 days. The result showed that body weight gain of control group (A) per week per rabbit was found to increase but in treated group B (arsenic trioxide@100ppm orally daily) the mean body weight of group B were decreased gradually and significant (P<0.05) decrease in body weight was observed at 40 days of feeding and at day 60 it was highly significant (P<0.01). Rabbits of group C, D and E were apparently normal and mild body weight reductions were observed which was statistically insignificant. No body weight loss was observed in rabbit of group F. TEC, Hb and PCV were slightly reduced in group C, D, E and F but highly significant (P< 0.01) reduction of TEC, Hb and PCV were observed in group B. ESR values were slightly increased in group C, D, E and F but highly significant (P< 0.01) increased of ESR value was observed in group B. Leukocytosis was observed in rabbits of group B. In this study, it was concluded that treatment with arsenic oxide at low doses has harmful effects on experimental animals including hematological alterations.

The present study described the cytopathic effect of PPR virus presently being used in serial passages at level of 60 th in Vero cells and infected tissue culture uid was used in this study as viral inoculum. Vero cells were grown on cover slip & were infected with tissue culture uid at a xed multiplicity of infection (MOI) 0.01. The infected cover slip along with control were stained with H&E stain at periodic intervals and cytopathic effect was studied with microscope. The cytopathic effect (CPE) was visible at rst from 24 hpi and the Vero cells showed initial cell rounding, aggregation and syncytial development. Development of inclusion bodies and cell degradation was noticed by 72 hpi. Complete detachment of the cell monolayer was observed by 84 hpi. It is concluded that, development of numerous inclusion bodies is the indication of well adaptation & extensive multiplication of PPRV in Vero cells. HighlightsLocally isolated Bangladeshi strain of peste des petits ruminants (PPR) virus adapted well in Vero cellsThe cytopathic effect (CPE) was visible at rst from 24-hour of post infection (hpi) and the Vero cells showed a sequence of changes includes -initial cell rounding, aggregation and syncytial development. The generalized CPE including large syncytia formation, development of more inclusion bodies. Complete cell degradation was noticed by 72 hrs post infection (hpi).However complete detachment of the cell monolayer was observed by 84 hrs post infection.Numerous acidophilic intracytoplasmic and intranuclear inclusion bodies indicated that the virus multiplied extensively in Vero cell.