The Effect of lead acetate alone and in combination with whole milk on body weight gain and some biochemical parameters were carried out on a total of 15 (15 days old) male weaning Long- Evans strain rats. The rats were randomly divided into three equal groups, each consisting of five rats. Rats of group A were kept as control (without giving any treatment), group B received lead acetate alone @ 6mg/ml drinking water and group C received lead acetate @ 6mg/ml plus whole milk (Star ship®) 150 mg/ml drinking water. The result showed that body weight gain of control group per week per rat was found to increase but in treated group B, the body weight gain was found to decrease most significantly (P< 0.01) on day 56 while in group C, body weight was reduced significantly (P< 0.05) on day 56. The reducing body weight gain was less in group C than group B. A most significantly (P< 0.01) increased SGOT and SGPT values were observed in Group B but in group C, those count increased significantly (P< 0.05) on day 56 of experiment. From the study it was concluded that treatment with lead acetate at low doses has adverse effects on body growth and liver functions in experimental animals.Asian J. Med. Biol. Res. June 2016, 2(2): 183-189

Effectiveness study of sulfaclozine as anticoccidial drug by counting litter oocyst were carried out on a total of 12 broiler farms at Sirajgonj District in Bangladesh. For this study 12 broiler farms were selected randomly and kept under close observation for the natural outbreaks of coccidiosis in a period of one broiler crop grow out. Poultry rearing and farm management systems followed in all farms were almost same. Litter samples were collected following same procedure in every week throughout the experimental period both before and after treatment with four (04) commercially available sulfaclozine Na preparations. The number of oocysts per gram (OPG) of litter was calculated following McMaster method. Huge number of oocyst was counted after outbreaks but before treatment of coccidiosis in almost every farm and after treatment oocyst were found significantly (P ˂0.01) reduced in number. From the study it was concluded that use of sulfaclozine Na in the treatment and prevention of coccidiosis was found still now effective.

Abstract:The effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and effects of sand-charcoal-iron-filter (SCIF)-bed filtrated water along with alum on body weight and some hematological parameters were carried out on a total of 30 (01 month old) adult Newzealand white rabbits. The Rabbits were randomly divided in to 6 equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100 ppm, group C received arsenic trioxide @ 100 ppm plus tannic acid @100 ppm, group D received arsenic trioxide @ 100 ppm plus di-sodium hydrogen phosphate @100 ppm, group E received arsenic trioxide @100 ppm plus alum @100 ppm orally daily for 60 days in all cases and group F received alum @100 ppm in SCIF-bed filtrated water orally daily for 60 days. The result showed that body weight gain of control group (A) per week per rabbit was found to increase but in treated group B (arsenic trioxide@100ppm orally daily) the mean body weight of group B were decreased gradually and significant (P<0.05) decrease in body weight was observed at 40 days of feeding and at day 60 it was highly significant (P<0.01). Rabbits of group C, D and E were apparently normal and mild body weight reductions were observed which was statistically insignificant. No body weight loss was observed in rabbit of group F. TEC, Hb and PCV were slightly reduced in group C, D, E and F but highly significant (P< 0.01) reduction of TEC, Hb and PCV were observed in group B. ESR values were slightly increased in group C, D, E and F but highly significant (P< 0.01) increased of ESR value was observed in group B. Leukocytosis was observed in rabbits of group B. In this study, it was concluded that treatment with arsenic oxide at low doses has harmful effects on experimental animals including hematological alterations.

The present study was undertaken for the detection of arsenic in different organs or tissues in an experimentally induced arsenicosis affected adult Newzealand white rabbits. The experiment was carried out on a total of 30 (01 month old) adult Newzealand white rabbits. Experimental arsenicosis were developed in rabbits by oral administration of arsenic trioxide alone and along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and As contaminated water after filtration using SCIF bed along with alum. For this, the rabbits were randomly divided in to six (6) equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100ppm, group C received arsenic trioxide @ 100ppm plus tannic acid @100ppm, group D received arsenic trioxide @ 100ppm plus di-sodium hydrogen phosphate (DSHP) @100ppm, group E received arsenic trioxide @100ppm plus alum @100ppm orally daily for 60 days in all cases and group F received alum@100ppm in SCIF-bed filtrated water orally daily for 60 days. The different organs and tissues of both dead and sacrificed rabbits (Liver, kidney, heart, Lungs spleen, stomach, muscle and skin) were collected for detection of arsenic. Arsenic was detected qualitatively by Reinsch test, semiquantitatively by Merck Arsen test kit. The distribution of arsenic concentration was highest in liver and lowest in skin. It has been concluded that arsenic were deposited in different organs or tissues of rabbit in an experimentally induced arsenicosis as like as natural occurrence.Asian J. Med. Biol. Res. September 2016, 2(3): 422-428