Effect of Lectins on the Transport of Food Factors in Caco-2 Cell Monolayers

Ohno, Yumiko; Naganuma, Takako; Ogawa, Tomoya; Muramoto, Koji

doi:10.1021/jf052040y

Cited by 35 publications

(24 citation statements)

References 43 publications

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“…In this study, ileal mucosa DAO in piglets fed 0.1, 0.15 or 0.2% SBA decreased, and plasma d -lactic acid content was enhanced, which indicated that the intestine injured by 0.1–0.2% SBA increased the epithelial permeability. A previous study showed that SBA (90 μg/mL) slightly increased the values with quercetin glycosides or calcium ions [18], which is consistent with this study.…”

Section: Discussionsupporting

confidence: 93%

Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets

Zhao

Qin

Sun

et al. 2011

IJMS

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This study was developed to provide further information on the intestinal barrier permeability and the tight junction protein expression in weaned piglets fed with different levels of soybean agglutinin (SBA). Twenty-five weaned crossbred barrows (Duroc × Landrace × Yorkshire) were selected and randomly allotted to five groups, each group with five replicates. The piglets in the control group were not fed with leguminous products. 0.05, 0.1, 0.15 and 0.2% SBA was added to the control diet to form four experimental diets, respectively. After the experimental period of 7 days (for each group), all the piglets were anesthetized with excess procaine and slaughtered. The d-lactic acid in plasma and the Ileal mucosa diamine oxidase (DAO) was analyzed to observe the change in the intestinal permeability. The tight junction proteins occludin and ZO-1 in the jejunum tissue distribution and relative expression were detected by immunohistochemistry and Western Blot. The results illustrated that a high dose of SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no significant affects. The contents of DAO, d-lactic acid, occludin or ZO-1, had a linear relationship with the SBA levels (0–0.2%) in diets. The high dose SBA (0.1–0.2%) could increase the intestinal permeability and reduce piglet intestinal epithelial tight junction protein occludin or ZO-1 expression, while low dose of SBA (0.05% of total diet) had no affects.

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Section: Discussionsupporting

confidence: 93%

Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets

Zhao

Qin

Sun

et al. 2011

IJMS

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“…7). These findings are in agreement with results published by Ohno et al [56], who found increases in the TER of Caco-2 monolayers after incubating them for 4 h with quercetin glycosides at 80 lM. However, other authors detected no significant effects of raspberry extracts containing various anthocyanins, ellagitannins, and hydroxycinnamic acid derivatives on the TER of Caco-2 monolayers [57].…”

Section: Influence Of Polyphenols On Tersupporting

confidence: 93%

The Ussing type chamber model to study the intestinal transport and modulation of specific tight‐junction genes using a colonic cell line

Bergmann

Rogoll

Scheppach

et al. 2009

Molecular Nutrition Food Res

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Polyphenols in apples, such as various hydroxycinnamic acids and flavonoids, have positive health effects that strongly depend on their bioavailability. In order to show that the Ussing-type chamber is a useful model to study metabolism, transport, and tightness of cell monolayers in one experimental setup, monolayers of the T84 colon carcinoma cell line mounted in Ussing-type chambers were incubated in the presence of physiological concentrations of various hydroxycinnamic acids (including ferulic, isoferulic, cinnamic, and hydrocinnamic acids) and flavonoids for 4 h. Concentrations of each tested polyphenol in the apical chamber, basolateral chamber, and those associated with the cells were then determined using HPLC with DAD (HPLC-DAD). The transport studies showed that the amounts of the tested polyphenols that passed from the apical to the basolateral side of the T84 monolayers depended on their polarity. Metabolites, such as glucuronides and sulfates of ferulic acid, were also detected at measurable levels by HPLC-ESI-MS/MS in the model system, but only when they were supplied at supra-physiological concentrations (>100 microM). In addition, the transepithelial resistance (TER) of T84 monolayers was measured before and after the addition of polyphenols, with and without short-term exposure to apical sodium caprate (C10), a tight junction (TJ) modulator. Exposure to C10 induced a decrease in TER that was reversible by incubation with polyphenols. However, no increase in paracellular permeability of tested polyphenols was observed after apical C10 exposure, so C10 did not promote fluxes of hydroxycinnamic acids across the monolayers. Further, real-time PCR analysis of the T84 colon cell line showed that ferulic and isoferulic acids induced significant increases in expression of the TJ components zonula occludens-1 (ZO-1) and claudin-4 transcription, but reductions in occludin expression. In contrast, caffeic and p-coumaric acids had no significant effects on the transcription of either ZO-1 or occludin. Our results provide confirmation that T84 cells could be used as model system to simulate the intestinal mucosa, and that polyphenols are able to increase the TER of C10-treated and -untreated T84 monolayers.

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“…It was also reported that garlic (A. sativum) lectin maintained its activity in the intestines and activated immunoreactivity when orally administered to mice (Clement et al, 2010). We have shown that lectins contained in foodstuff had modulating effects on the transport system of human intestinal Caco-2 cell monolayers (Ohno et al, 2006;Yamamoto et al, 2013;Nemoto et al, 2015). Thus, plant lectins can be considered as physiologically active ingredients in food resources.…”

Section: Discussionmentioning

confidence: 79%

Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves

Yamazaki

Ogawa

Muramoto

et al. 2016

FSTR

Self Cite

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Proteins contained in the green leaf mucus of Japanese bunching onion (Allium fistulosum L.) were isolated by ammonium sulfate precipitation and separated by hydrophobic interaction chromatography. The mucus proteins had an enhanced effect on tumor necrosis factor (TNF)-α production in the RAW 264 murine macrophage cell line. Protein composition was analyzed by SDS-polyacrylamide gel electrophoresis and N-terminal amino acid sequencing.The effect on RAW 264 cells was mainly attributed to mannose-binding lectin and thaumatin-like proteins, which occupied 34% of the mucus proteins. Other major proteins contained in the mucus showed homologous amino acid sequences to those of lachrymatory factor synthase, β-1,3-glucanase, 2S albumin precursor, and pathogenesis-related protein 4. Protein composition ratios in the mucus of 13 cultivars of Japanese bunching onion were also examined.Taken together, the green leaf mucus of Japanese bunching onion may be a possible source of various bioactive proteins.

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Effect of Lectins on the Transport of Food Factors in Caco-2 Cell Monolayers

Cited by 35 publications

References 43 publications

Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets

Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets

The Ussing type chamber model to study the intestinal transport and modulation of specific tight‐junction genes using a colonic cell line

Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves

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