Effect of lentinan on macrophage cytotoxicity against metastatic tumor cells

Ladányi, Andrea; Tı́már, József; Lapis, K.

doi:10.1007/bf01754412

Cited by 60 publications

(22 citation statements)

References 15 publications

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“…In addition, lentinan administration has been shown to induce generation of cytotoxic T cells and NK cells, stimulating their anti-cancer activities 16 . Furthermore, lentinan was shown to stimulate cytotoxicity of macrophages against metastatic tumors in mouse studies 30 . Thus, lentinan-stimulated immunity is a key mechanism responsible for its anti-cancer properties.…”

Section: Discussionmentioning

confidence: 99%

Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Ahn

Jeon

Kim

et al. 2017

Sci Rep

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Lentinan extracted from shiitake (Lentinula edodes) is a β-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. In this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloid cells. Mouse bone marrow-derived macrophages were treated with lentinan with/without inflammasome triggers, and maturation of interleukin (IL)-1β, IL-18, or caspase-1 was measured as a readout of inflammasome activation. As a result, lentinan selectively inhibited absent in melanoma 2 (AIM2) inflammasome activation. In addition, lentinan up-regulated pro-inflammatory cytokines and induced expression of inflammasome-related genes through toll-like receptor 4 signaling. Furthermore, we assessed the effect of lentinan on mice treated with Listeria monocytogenes or lipopolysaccharide as an AIM2 or non-canonical inflammasome-mediated model. Lentinan attenuated IL-1β secretion resulting from Listeria-mediated AIM2 inflammasome activation and reduced endotoxin lethality via inhibition of non-canonical inflammasome activation. Thus, lentinan is suggested as an anti-AIM2 and anti-non-canonical inflammasome candidate despite its up-regulation of cytokine expression.

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Section: Discussionmentioning

confidence: 99%

Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Ahn

Jeon

Kim

et al. 2017

Sci Rep

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“…It is well known that polysaccharides enhance various immune responses in vivo and in vitro (Ladányi et al, 1993;Lee & Jeon, 2003;Liu et al, 1996;Tzianabos, 2000). In addition, many polysaccharides activated immune responses first by activation of macrophages (Lee & Jeon, 2005).…”

Section: Introductionmentioning

confidence: 99%

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Chen

Zheng

2014

Pharmaceutical Biology

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Context: Atractylodes macrocephala Koidz is a traditional herb. Atractylodes macrocephalaon polysaccharides (AMP) have been found to enhance immunity and improve heart function. However, the mechanisms of the immunomodulatory effect have not been investigated. Objective: We examined whether AMP activated macrophages and explored the mechanisms of activation. Materials and methods: AMP was prepared and evaluated its immunomodulatory activity (25, 50, 100, and 200 lg/mL) by detecting the phagocytosis and the production of tumor necrosis factor-a (TNF-a), IFN-c, and nitric oxide (NO) in RAW264.7 macrophages. Furthermore, the role of nuclear factor-kB (NF-kB) pathway was examined in regulating TNF-a and NO production. Results: The phagocytosis of macrophages was enhanced by AMP in a dose-dependent manner and the maximal phagocytosis of macrophages occurred at concentrations of 100 and 200 lg/mL. NO, TNF-a, and IFN-c release was also found to be dose dependent by increasing concentrations of AMP and reached the peak at a concentration of 200 lg/mL. In addition, AMP induced inhibitor kappaB (IkB) degradation and the activation of NF-kB by p65 nuclear translocation, and then the activation of NF-kB in nucleus peaked at a concentration of 200 lg/ mL. Besides, NF-kB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) decreased AMP-induced NO and TNF-a production. Discussion and conclusion: These data suggest that AMP may modulate macrophage activities by stimulating NF-kB or activating NF-kB-dependent mechanisms.

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“…Lentinan is currently used clinically as an antitumor agent (18,19). It has been shown to stimulate natural killer cell activity (20 -22), macrophage/monocyte functions (secreting IL-1 and superoxide anion), phagocytosis, and cytotoxicity (23)(24)(25)(26)(27)(28). Lentinan was found to elevate the cytotoxic activity and TNF secretion of macrophages in vitro and in vivo (16,29).…”

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confidence: 99%

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

Pan

Zhang

et al. 2011

Journal of Biological Chemistry

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Lentinan, a cell wall ␤-glucan from the fruiting bodies of Lentinus edodes, is well known to be a biological defense modifier, but the signal transduction pathway(s) induced by Lentinan have not been elucidated. In this study, we extracted Lentinan (LNT-S) by ultrasonication from Lentinus edodes and report that, in murine RAW 264.7 macrophages, LNT-S glucan activated NF-B p65 and triggered its nuclear translocation as determined by Western blotting. Moreover, LNT-S enhanced NF-B-luciferase activity in the Dual-Luciferase gene system assay. Its upstream signaling molecules, MAPKs such as ERK1/2 and JNK1/2, were shown to be activated by assessing the level of phosphorylation in a time-and concentration-dependent manner, but its downstream proinflammatory enzyme, inducible NOS, was not observed. The data evaluated using a TNF-␣ ELISA kit and Griess reagent further demonstrated that no proinflammatory mediators such as TNF-␣ and NO were produced by LNT-S stimulation in RAW 264.7 cells. In contrast, LPS significantly induced inducible NOS expression and increased NO and TNF-␣ production, which are associated with activation of the NF-B p65/p50 heterodimer complex. It is possible that LNT-S did not activate NF-B p65/p50, and the activation of NF-B p65 was not sufficient to stimulate cytokine production. These data demonstrate that LNT-S glucan carries out its immunomodulating activity by activating MAPK signaling pathways without secretion of TNF-␣ and NO.

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Effect of lentinan on macrophage cytotoxicity against metastatic tumor cells

Cited by 60 publications

References 15 publications

Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Immunomodulatory β-Glucan from Lentinus edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages

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