Effect of leptin gene polymorphism on growth and carcass traits in pigs (short communication)

Kulig, H.; Grzesiak, Wilhelm; Szatkowska, Iwona

doi:10.5194/aab-44-291-2001

Cited by 11 publications

(21 citation statements)

References 16 publications

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“…The same allele frequencies were found by Borges and Goulart (2002), Stratil et al (1997), Korwin-Kossakowska et al (2001), Kulig et al (2001), and Szydlowski et al (2004) when studying other pig breeds.…”

Section: Discussionsupporting

confidence: 79%

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Silveira¹,

Antunes²,

Almeida³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fragment length polymorphism using the restriction enzyme HinfI. The data were statistically analyzed by the chi-square test. The results showed that 87.5% of the animals were genotype TT, where 31.25% were Large White paternal lineage, 31.25% were Pietrain paternal lineage and 25% were Pietrain maternal lineage. The TC genotype appeared in 12.5%, where 10% were Large White, 0.5% were Pietrain paternal lineage and 2% were of the same maternal lineage breed; CC was not observed. As for the allele frequency, 93.75% of the T and 6.25% of the C allele were found. In Pietrain maternal and paternal lineages, it could not be determined that the Obese gene had been fixed in the maternal lineage. However, in the Pietrain and Large White paternal lineages there was a statistically significant difference, suggesting that the C allele is associated with growth and daily weight gain and that the low frequency of C in the Pietrain lineage is related to low rate of body fat, a typical feature of this breed.

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Section: Discussionsupporting

confidence: 79%

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Silveira¹,

Antunes²,

Almeida³

et al. 2008

Genet. Mol. Res.

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show abstract

“…A similar, also higher frequency of LEP T was observed by STRATIL et al (1997) who studied the breeds Landrace, Hampshire, Large White, Black Pied Pŏeštice, Pietrain, and Czech Meat Pig; however, the allele was not found in Meishan pigs. A higher frequency of the allele LEP T was also revealed in Polish Landrace pigs (KULIG et al, 2001). Considering the mentioned data on leptin gene expression, leptin function, a high degree of the hormone's homology and its receptors location, one may suggest there is an effect of the protein on, among others, reproduction-related traits of pigs or, more specifically, on boar semen characters.…”

Section: Resultsmentioning

confidence: 99%

“…A realtionship was found between the C/T (3469) polymorphism and the carcass leannes as well as the daily gain of Polish Landrace pigs. The values of these traits were statistically significantly higher in TC individuals compared with TT homozygotes (KULIG et al, 2001). However, different results were reported by KENNES et al, 2001, for the Landrace breed.…”

Section: Introductionmentioning

confidence: 85%

Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars

2003

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SummaryStudies on porcine genome contributed to identification of polymorphic loci of the genes that may influence the level of reproduction-related traits. The LEP gene codes for leptin -a protein secreted to blood from adipocytes, important for food intake, energy expenditure, and immune response; the protein also influences some functions of mammalian reproductive system. The aim of this study was to determine the polymorphism of HinfI in the leptin gene and its effect on selected qualitative and quantitative traits of the semen. The analyses included 54 AI boars, which had been derived from crossing Duroc with Pietrain breeds. The genotypes were established with PCR-RFLP. Two alleles LEP/HinfI were identified: LEP T (152 bp) and LEP C (68 and 84 bp) with the frequencies 0.713 and 0.287 respectively. The genotype frequencies were as follows:

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“…However, the CC homozygotes were not identified. The low frequency of homozygotes with C allele to the level of 0.15 was detected in the studies of Kulig et al (2001), where TT genotype appeared up to the level of 0.76 and showed to be the predominant genotype. Low frequency of C allele in Duroc, Yorkshire and Landrace also resulted from the research of Kennes et al (2001).…”

Section: Discussionmentioning

confidence: 97%

Effect of Leptin Gene Polymorphism on Fattening and Slaughter Value of Line 990 Gilts

2009

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The study was aimed at defining leptin gene polymorphism and its potential association with values of particular features of fattening and slaughter value of gilts of Line 990. The study included a total of 208 gilts. The polymorphic locus in LEP gene was identified by the restriction enzyme HinfI in 3469 position, by using the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method. Two alleles of LEP gene were identified: T (0.94) and C (0.06), resulting in two genotypes: TC (0.12) and TT (0.88). We did not observe any gilts of CC genotype. The analysis of values of fattening and slaughter features, depending on LEP genotype did not reveal significant differences in body mass increase, daily gain from day 63 to 180, and daily gain from birth to day 180, feed conversion per 1 kg body mass and the loin-eye thickness. Significant differences between the LEP genotypes were present for such features as the backfat thickness at points P2 (p ≤ 0.05) and P4 (p ≤ 0.01) and average backfat thickness (p ≤ 0.01) in favour of TT genotype. We noted higher average values of lean meat content in carcass in favour of TT homozygotes, compared to the heterozygotes (p ≤ 0.05). The investigation contributes additonal information regarding LEP gene polymorphism in gilts of Line 990. Knowledge of LEP genotypes may be useful to improve the slaughter value in gilts primarily due to less fatness. Due to the lack of individuals representing CC genotype in our results, research should continue on a larger population.

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Effect of leptin gene polymorphism on growth and carcass traits in pigs (short communication)

Cited by 11 publications

References 16 publications

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars

Effect of Leptin Gene Polymorphism on Fattening and Slaughter Value of Line 990 Gilts

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Effect of leptin gene polymorphism on growth and carcass traits in pigs (short communication)

Cited by 11 publications

References 16 publications

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

Preliminary results on associations between leptin gene (&lt;i&gt;LEP&lt;/i&gt;) and some reproduction performance traits of boars

Effect of Leptin Gene Polymorphism on Fattening and Slaughter Value of Line 990 Gilts

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Preliminary results on associations between leptin gene (<i>LEP</i>) and some reproduction performance traits of boars