Effect of leukocytospermia on fertilization and pregnancy rates of artificial reproductive technologies

Lackner, Jakob; Märk, Isabel; Sator, K.; Huber, Johannes; Sator, Michael

doi:10.1016/j.fertnstert.2007.07.1310

Cited by 37 publications

(37 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most importantly, moderate leukocytospermia was associated with an improved chance of having a successful pregnancy by ART. These data are more complete and more significant but in agreement with those reported by Lackner et al (23), who studied a group of 75 couples and found that leukocytospermia may not necessarily have a negative effect on outcome after either IVF or ICSI.…”

Section: Discussionsupporting

confidence: 91%

Seminal leukocytes are Good Samaritans for spermatozoa

Barraud-Lange

Pont

Ziyyat

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Seminal leukocytes are Good Samaritans for spermatozoa

Barraud-Lange

Pont

Ziyyat

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

“…Therefore, casual correlation is to be excluded. On the other hand, it has been observed that leukocytes do not always have a negative effect on semen quality and ART outcome [32]. Our findings may support these data.…”

Section: Discussionsupporting

confidence: 90%

“…Leukocytes have been reported to negatively influence sperm-egg fusion in experiments [8] and fertilization rate in both IVF and ICSI cycles [14,30-32]. Antibiotic and anti-inflammatory treatment have been suggested for asymptomatic leukocitospermia, but the efficacy of such therapies remains to be definitely established [33-35].…”

Section: Discussionmentioning

confidence: 99%

Leukocytospermia and sperm preparation - a flow cytometric study

Ricci

Perticarari

Boscolo

et al. 2009

Reprod Biol Endocrinol

View full text Add to dashboard Cite

BackgroundLeukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i) to compare the efficiency of swim-up and density-gradient centrifugation techniques in removing seminal leukocytes, (ii) to examine the effect of leukocytes on sperm preparation, and (iii) to compare flow cytometry and peroxidase test in determining leukocyte concentration in semen using a multiparameter flow cytometric method.MethodsSemen samples from 126 male partners of couples undergoing infertility investigations were analyzed for leukocytospermia using standard optical microscopy and flow cytometry. Sixty-nine out of 126 samples were also processed using simultaneously the swim-up and density-gradient centrifugation techniques. A multiparameter flow cytometric analysis to assess simultaneously sperm concentration, sperm viability, sperm apoptosis, and leukocyte concentration was carried out on neat and prepared sperm.ResultsBoth sperm preparation methods removed most seminal leukocytes. However, the concentration of leukocytes was significantly lower after swim-up compared to that after density-gradient centrifugation preparation. Leukocytes concentration, either initial or in prepared fractions, was not correlated with sperm parameters (optical microscopy and flow cytometry parameters) after semen processing. There was no correlation between leukocyte concentration in the ejaculate and sperm recovery rate, whereas a significant correlation was found between the concentration of the residual leukocytes in prepared fractions and viable sperm recovery rate. Although the overall concordance between the flow cytometry and the optical microscopy was satisfactory, the sensitivity of peroxidase test for the detection of leukocytospermia resulted low.ConclusionSeminal leukocytes do not seem to influence sperm preparation results. However, for assisted conception, semen samples containing leukocytes should be processed using swim-up method. Although peroxidase-test is recommended by WHO as the standard method for determining semen leukocytes, it should not be used in clinical research study.

show abstract

“…Une seule publication de Lackner et al [17] avait suggé ré , sur un petit nombre de tentatives d'AMP, que la leucospermie n'é tait pas forcé ment un é lé ment pé joratif en soit. C'est l'é tude de Barraud-Lange et al [18] qui a eu l'inté rêt d'é tudier de maniè re plus approfondie le problè me de la leucospermie sur un grand nombre de cas.…”

Section: La Prostate Pathologiqueunclassified