Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

Abstract

The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose.

Effect of light intensity and seal type on the in vitro elongation and adventitious rooting of Eucalyptus grandis × E. urophylla

“…Number of shoots per explant was highest under the R/B 20 and 0/M combination, which contrast with variables related to microstump growth and development which were highest under the FL 40 and 1/M combination. Some studies have shown that a light source with a broad light spectrum induces greater in vitro plant development (Batista et al 2018;Santos et al 2020;Miranda et al 2020).…”

“…The control of spectral quality, light intensity, and gas exchange during plant culture is essential to increase photosynthetic rates and shoot development R/B 40 µmol m -2 s -1 without a porous membrane. However, as a general rule it seems that membranes allowing gas exchange have a positive effect on in vitro propagation, likely to be associated to regular supply of CO 2 and O 2 , lower relative humidity and lower ethylene concentration inside the culture vessel (Hung et al 2016, Souza et al 2019Santos et al 2020).…”

Optimizing Photoautotrophic Micropropagation Conditions for Ginger

“…To simplify this process, this study divided the successive cluster seedlings of ginger into single plants, and the eld seedlings were directly established using photoautotrophic micropropagation technology. Photoautotrophic micropropagation helps the acclimatization phase, improving the anatomical and physiological characteristics that allow seedling survival and development (Santos et al 2020). The aim of this study was to develop a reliable and photoautotrophic micropropagation system that would improve the quality of ginger tissue culture seedlings and increase the survival rate during transplantation.…”

Led enhance the growth characteristics and affects the ex vitro aclimatization of sugarcane

“…Several studies report the advantage of using natural ventilation and forced ventilation with CO 2enrichment. For natural ventilation, studies with Pfaffia glomerata (Iarema et al, 2012;Saldanha et al, 2012;Corrêa et al, 2015a;Corrêa et al, 2015b), Capsicum annuum (Batista et al, 2017), Physalis angulata (Santos et al, 2020), Acca sellowiana (Caetano et al, 2021), Capsicum frutescens (Gris et al, 2021), Epidendrum fulgens (Fritsche;Pinheiro;Guerra, 2022), Guazuma ulmifolia (Jesus-Santana et al, 2022), for forced ventilation, studies with Pfaffia glomerata (Saldanha et al, 2013;Saldanha et al, 2014;Ferreira et al, 2019), Lippia alba (Batista et al, 2017), Etlingera elatior (Pinheiro et al, 2021), among others. These studies brought the benefits of the use of gas-porous membranes, which provides a reduction in relative humidity and ethylene gas inside the flasks, which can eliminate or drastically reduce morphophysiological disorders and increase survival during acclimatization.…”