Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro

Abstract

The objective of this study was to determine the effects of different light-emitting diode (LED) light sources on the growth of upland cotton (Gossypium hirsutum L.) plantlets. Shoot bud apex cuttings of upland cotton (1.0 cm) were transplanted on Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/l 6-benzyladenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA) and cultured in vitro for 45 days. They were exposed to 50 lmol m -2 s -1 photosynthetic photon flux (PPF) and a 12-h photoperiod under six different lights: fluorescent lamp (CON), monochromatic blue LED (B), three blue and red LED mixtures (B:R = 3:1, 1:1, 1:3) and monochromatic red LED (R). The effects of the six light sources on growth and morphogenesis of upland cotton plantlets grown in vitro were investigated. Fresh weight, dry weight, stem length and second internode length were greatest in plantlets cultured under the B:R = 1:1 blue and red LED light, followed by blue LED light, and they were lowest in plantlets cultured under a fluorescent lamp. Chlorophyll content, leaf thickness, palisade tissue length, leaf and stomata area were highest in plantlets cultured under blue LED light. Root activity, sucrose, starch and soluble sugar contents were highest in plantlets cultured under red LED light. Our results showed that larger, healthier plantlets and a greater biomass of upland cotton were produced in the presence of red LED supplemented with a quantity of blue LED light. Blue and red LED (B:R = 1:1) was the most suitable light for the growth of upland cotton plantlets in vitro, and it may be used as alternative light source for an upland cotton culture system.

Light‐Emitting Diodes: Progress in Plant Micropropagation

“…Jao et al [18] reported that blue LEDs promote growth and increase chlorophyll content in Zantedeschia jucunda. The same effect was observed by Li et al [19,20] during in vitro culture of Gossypium hirsutum and Brassica campestris, respectively. Kim et al [9] and Moon et al [21] emphasized the role of blue light on chlorophyll formation and chloroplast development in their work with Chrysanthemum and Tripterospermum japonicum, respectively.…”

New light sources for in-vitro potato micropropagation

“…Several studies have been made to optimize the micropropagation process, mainly related to culture medium composition (ABDULLATEEF et al, 2009). However, tissue culture environments might be improved by using new available light sources that might represent lower production costs, especially for seed potato production (SEABROOK, 2005;LI et al, 2010).…”

“…Recently developed light emission diodes (LEDs) have been pointed out by authors as potential sources of light for in-vitro environments cultivation. LEDs are characterized by specific wavelengths, small mass and volume, long useful life, low heating and highly efficient light generation process (60%), and do not contain mercury or other hazard element to the environment (YEH;CHUNG, 2009 ( NHUT et al, 2002), strawberry (ROCHA et al, 2010), cotton (LI et al, 2010), among others. This work aimed to optimize the in-vitro propagation of three potato cultivars, using new light sources in the culture environment.…”

The effect of radiation of LED modules on the growth of dill (Anethum graveolens L.)

“…A considerable reduction in the length of hypocotyl was noticed when blue light proportion reached 10% . It is known that blue light is important for leaf expansion and it enhances the leaf area and biomass production [5,23]. In our study, combination C (50/30) was characterised by the greatest fresh mass of plants in the last week.…”