Effect of light exposure on linalool biosynthesis and accumulation in grape berries

Sasaki, Kenji; Takase, Hideki; Matsuyama, Shuhei; Kobayashi, Hironori; Matsuo, Hironori; Ikoma, Gen; Takata, Ryoji

doi:10.1080/09168451.2016.1217148

Cited by 39 publications

(22 citation statements)

References 21 publications

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“…The content of the monoterpene linalool and the expression of its biosynthetic genes were reduced in shaded or UV-filtered berries of cv. Riesling (Sasaki et al, 2016). The authors also showed that light reflection was sufficient for an increase in linalool concentration.…”

Section: A Metabolic Grid Of Diverse Compounds Induced By Different Tmentioning

confidence: 99%

Transcriptomic and Metabolomic Networks in the Grape Berry Illustrate That it Takes More Than Flavonoids to Fight Against Ultraviolet Radiation

Matus

2016

Front. Plant Sci.

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Plants are constantly challenged by environmental fluctuations. In response, they have developed a wide range of morphological and biochemical adaptations committed to ameliorate the effects of abiotic stress. When exposed to higher solar radiation levels, plants activate the synthesis of a large set of enzymes and secondary metabolites as part of a complex sunscreen and antioxidant defense mechanism. Grapevine (Vitis vinifera L.) has become a widely used system for studying adaptive responses to this type of stress since changes in berry composition, positively influenced by increased ultraviolet (UV) radiation levels, improve the quality of wines subsequently produced. Despite the fact that most of the attention has been directed toward the synthesis of flavonoids, recent transcriptomic and metabolomic studies have shown that stilbenoids and isoprenoids (e.g., terpenes and carotenoids) are also an important part of the grape UV-response machinery. This minireview focuses on the latest findings referring to the metabolic responses of grapes to UV radiation and proposes a model for its transcriptional control. Depending on the berry developmental stage and the type of radiation (i.e., irradiance level, exposure length), increased UV levels activate different metabolic pathways through the activity of master regulators belonging to the basic Leucine Zipper Domain (bZIP) and R2R3-MYB transcription factor families. This transcriptional control is influenced by the interaction of other environmental factors such as light, temperature or soil water availability. In grapevine, phenylpropanoids are part of, but are not the whole story, in the fight against radiation damage.

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Section: A Metabolic Grid Of Diverse Compounds Induced By Different Tmentioning

confidence: 99%

Transcriptomic and Metabolomic Networks in the Grape Berry Illustrate That it Takes More Than Flavonoids to Fight Against Ultraviolet Radiation

Matus

2016

Front. Plant Sci.

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“…Inconsistent with previous reports, VvDXR and VvHDR expression were not detected throughout the ripening stages in this study. Similarly, the transcript abundance of VvCSLinNer gene, which was reported as the key gene involved in linalool accumulation 8,12 , was very low throughout the ripening period except the onset of véraison, but was uncorrelated with linalool accumulation. The similar low expression levels of VvCSLinNer gene were found in ripening berries of other cultivars 11,43,44 .…”

Section: Discussionmentioning

confidence: 95%

“…Moreover, many of terpene synthases (TPSs) involved in late steps of monoterpenols biosynthesis have been functionally characterized 10,11 . Among them, the linalool/nerolidol synthase gene VvCSLinNer was reported to play an important role in linalool accumulation 8,12 .The effects of agronomic practices such as leaf removal, irrigation, and exogenous hormones on primary and secondary metabolism in grapes have been well studied during the past decades 3 . Regulation of the source/sink ratio via cluster thinning is a common practice to enhance the accumulation of secondary metabolites, especially flavonols and anthocyanins [13][14][15][16][17][18] .…”

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confidence: 99%

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Influence of cluster thinning and girdling on aroma composition in ‘Jumeigui’ table grape

Zha

et al. 2020

Sci Rep

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Cluster thinning and girdling are common and simple practices applied to improve berry quality in table grape cultivation. However, there is limited information about the accumulation and biosynthesis of the entire aromatic profile under cluster thinning and girdling, notably in table grapes. This research investigated the influences of cluster thinning and girdling (alone or in combination) on aroma profiles, particularly the changes in biosynthesis and accumulation of Muscat-flavored related compounds from véraison to harvest in 'Jumeigui' grape. Cluster thinning and girdling (alone or in combination) significantly increased the concentrations of total soluble solids (TSS) and key aromatic compounds at harvest, with higher concentrations of both under cluster thinning than girdling. Berry weight and titratable acidity (TA) were unaffected by cluster thinning, girdling, or in combination at harvest. Linalool, the most abundant and active odorant related to Muscat flavor, accumulated in 28.6% and 20.2% higher concentrations from cluster thinning than control and girdling at maturity, respectively. Furthermore, higher DXS3 transcript abundance in cluster thinning groups might contribute to the increased accumulation of terpenes and linalool in 'Jumeigui' grape. The results will contribute to further understand the mechanism of source/sink ratio modulation on aroma accumulation and better apply cluster thinning and girdling for grape production.Aroma is one of the important indicators contributing to table grape and wine quality 1,2 . Although several hundred aroma compounds have been identified, the most important families of compounds responsible for the aroma of grapes are terpenes, C 13 norisoprenoids, methoxypyrazines, C 6 alcohols and aldehydes, esters, and thiols 1,3 . Aroma compounds in grapes exist as free and bound glycosides. In table grapes, free forms might be the only vital ingredients to determine the flavor despite odourless bound glycosylated forms could be hydrolyzed to odour-active free forms during fermentation 4,5 .Monoterpene alcohols, notably linalool, with low perception thresholds, contribute to floral characters and are particularly prevalent in Muscat flavored varieties. Monoterpenes in grapevine berries have been demonstrated to biosynthesize via the plastidial 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway 6 . Due to the correlation between gene expression and monoterpenes accumulation, 1-deoxy-D-xylulose 5-phosphate (DXP) synthase (DXS), DXP reductoisomerase (DXR), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) and geranyl pyrophosphate synthase (GPPS) are putative upstream rate-limiting enzymes 7-9 . Moreover, many of terpene synthases (TPSs) involved in late steps of monoterpenols biosynthesis have been functionally characterized 10,11 . Among them, the linalool/nerolidol synthase gene VvCSLinNer was reported to play an important role in linalool accumulation 8,12 .The effects of agronomic practices such as leaf removal, irrigation, and exogenous hormones on primary and ...

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“…Sunlight exclusion limits the synthesis and accumulation of terpenes also in grape berries (linalool and the bound forms being the most responsive) by especially affecting DXS and TPS genes (Zhang et al, 2014; Friedel et al, 2016; Joubert et al, 2016; Matus, 2016; Sasaki et al, 2016). Our findings (Figure 4, Table 2, and Supplementary Table S9) are consistent with a role, among others, for HY5 ( LONG HYPOCOTYL5 , VIT_04s0008g05210) in the regulation of light-induced terpenoid biosynthesis in grapes, in agreement with other evidences (Carbonell-Bejerano et al, 2014a,b; Zhou et al, 2015; Loyola et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Drawing Links from Transcriptome to Metabolites: The Evolution of Aroma in the Ripening Berry of Moscato Bianco (Vitis vinifera L.)

et al. 2017

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Monoterpenes confer typical floral notes to “Muscat” grapevine varieties and, to a lesser extent, to other aromatic non-Muscat varieties. Previous studies have led to the identification and functional characterization of some enzymes and genes in this pathway. However, the underlying genetic map is still far from being complete. For example, the specific steps of monoterpene metabolism and its regulation are largely unknown. With the aim of identifying new candidates for the missing links, we applied an integrative functional genomics approach based on the targeted metabolic and genome-wide transcript profiling of Moscato Bianco ripening berries. In particular, gas chromatography-mass spectrometry analysis of free and bound terpenoid compounds was combined with microarray analysis in the skins of berries collected at five developmental stages from pre-veraison to over-ripening. Differentially expressed metabolites and probes were identified in the pairwise comparison between time points by using the early stage as a reference. Metabolic and transcriptomic data were integrated through pairwise correlation and clustering approaches to discover genes linked with particular metabolites or groups of metabolites. These candidate transcripts were further checked for co-localization with quantitative trait loci (QTLs) affecting aromatic compounds. Our findings provide insights into the biological networks of grapevine secondary metabolism, both at the catalytic and regulatory levels. Examples include a nudix hydrolase as component of a terpene synthase-independent pathway for monoterpene biosynthesis, genes potentially involved in monoterpene metabolism (cytochrome P450 hydroxylases, epoxide hydrolases, glucosyltransferases), transport (vesicle-associated proteins, ABCG transporters, glutathione S-transferases, amino acid permeases), and transcriptional control (transcription factors of the ERF, MYB and NAC families, intermediates in light- and circadian cycle-mediated regulation with supporting evidence from the literature and additional regulatory genes with a previously unreported association to monoterpene accumulation).

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Effect of light exposure on linalool biosynthesis and accumulation in grape berries

Cited by 39 publications

References 21 publications

Transcriptomic and Metabolomic Networks in the Grape Berry Illustrate That it Takes More Than Flavonoids to Fight Against Ultraviolet Radiation

Transcriptomic and Metabolomic Networks in the Grape Berry Illustrate That it Takes More Than Flavonoids to Fight Against Ultraviolet Radiation

Influence of cluster thinning and girdling on aroma composition in ‘Jumeigui’ table grape

Drawing Links from Transcriptome to Metabolites: The Evolution of Aroma in the Ripening Berry of Moscato Bianco (Vitis vinifera L.)

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