Effect of light on germinating spores of Streptomyces viridosporus

Imbert, M

doi:10.1016/s0378-1097(99)00530-3

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…S. coelicolor spores contain endogenous sources of nutrients such as trehalose (19,20), and it may be that growth arrest is a manifestation of a switch by the extending tip from an endogenous to an exogenous energy source or even a redeployment of nutrient resources within the microcolony. We were unable to generate movies of FtsZ-EGFP during germination, which is perhaps due to the inhibitory effects of light during spore germination seen in some streptomycete species (15). Following germination, hyphal extension occurred at a rate of ϳ20 m h Ϫ1 and FtsZ-EGFP rings were laid down ϳ56 m behind the growing tip.…”

Section: Discussionmentioning

confidence: 99%

Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

Jyothikumar

Tilley

Wali

et al. 2008

Appl Environ Microbiol

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Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a result, in order to study the mechanisms that underpin streptomycete growth, we have developed a system for studying hyphal extension, protein trafficking, and sporulation by time-lapse microscopy. Through the use of time-lapse microscopy we have demonstrated that Streptomyces coelicolor germ tubes undergo a temporary arrest in their growth when in close proximity to sibling extension sites. Following germination, in this system, hyphae extended at a rate of ϳ20 m h ؊1 , which was not significantly different from the rate at which the apical ring of the cytokinetic protein FtsZ progressed along extending hyphae through a spiraling movement. Although we were able to generate movies for streptomycete sporulation, we were unable to do so for either the erection of aerial hyphae or the early stages of sporulation. Despite this, it was possible to demonstrate an arrest of aerial hyphal development that we suggest is through the depolymerization of FtsZ-enhanced green fluorescent protein (GFP). Consequently, the imaging system reported here provides a system that allows the dynamic movement of GFP-tagged proteins involved in growth and development of S. coelicolor to be tracked and their role in cytokinesis to be characterized during the streptomycete life cycle.Fluorescence microscopy has revolutionized our understanding of the bacterial cell and provided new opportunities to investigate the behavior of cell division proteins and chromosome dynamics in bacteria (25). Central to this research is the application of time-lapse microscopy to study bacterial cell division, which has revealed the complexity with which bacteria coordinate cellular growth and division. For example, the rodshaped bacteria Escherichia coli and Bacillus subtilis incorporate new peptidoglycan into their cell wall along their lateral walls, while coccoid bacteria such as Staphylococcus aureus do so at mid-cell (4). Actinobacteria, such as Corynebacterium and members of the mycelial, antibiotic-producing genus Streptomyces, incorporate peptidoglycan at the cell poles (4). In the case of streptomycetes, this allows them to adopt a hyphal growth strategy through peptidoglycan incorporation at the hyphal tip (9). This is ideally suited to the colonization of their particulate habitat, the soil, through the generation of a mycelium that permits nutrients to be transported from a nutrient reservoir to the actively growing tip. As such, streptomycetes represent a group of organisms that grow in a fashion distinct from other, better understood bacteria. The knowledge base associated with morphological and physiological differentiation in the model organism Streptomy...

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Section: Discussionmentioning

confidence: 99%

Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

Jyothikumar

Tilley

Wali

et al. 2008

Appl Environ Microbiol

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“…On the other hand, S. mycarofaciens SS‐2‐243 had a significantly higher growth rate in the dark condition than in the light condition. This was due to the fact that light remarkably inhibited the spore germination as also found in S. viridosporus and S. coelicolor [40]. The influence of light on the growth of various Streptomyces spp.…”

Section: Discussionmentioning

confidence: 98%

“…S. phaeopurpureus 5125, S. salmonicida 5472, and S. fulvissimus could grow well in light conditions [27]. On the other hand, S. viridosporus, S. lividans , and S. coelicolor were sensitive to light, while S. ambofaciens and S. pilosus remained unchanged and unaffected [40]. It has been demonstrated that light conditions influenced the growth of Streptomyces spp.…”

Section: Discussionmentioning

confidence: 99%

Impact of environmental factors on Streptomyces spp. metabolites against Botrytis cinerea

et al. 2022

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Botrytis cinerea is an economically important disease on numerous vegetables including tomato. From our previous studies, a spore suspension of Streptomyces philanthi RL-1-178 and RM-1-138 and Streptomyces mycarofaciens SS-2-243 showed strong inhibition against B. cinerea. In this study, the efficacy of their antifungal metabolites against B. cinerea was investigated after enhancing the production through the optimum culture medium and environmental conditions (temperature, light/dark cycle). In vitro studies indicated that glucose yeast-malt (GYM) agar and incubation at 28°C were optimal for growth and mass spore production of all three Streptomyces strains. Moreover, light/dark conditions had a positive effect on the growth and spore production of S. philanthi RM-1-138 and RL-1-178 but not on S. mycarofaciens SS-2-243.Both strains of S. philanthi possessed an antifungal activity against B. cinerea (100% inhibition) while S. mycarofaciens showed different results on PDA (83% inhibition) and GYM (88% inhibition) at the optimum incubation temperature at 21°C. The antifungal compounds from S. philanthi RM-1-138 exhibited the highest protection efficacy against B. cinerea on tomato leaves (82.89% and 0.33 cm 2 lesion areas symptoms). The antifungal compounds RM-1-138, identified by GC-MS, were greatly altered based on components concentration under various temperatures and light/dark conditions. The anti-B. cinerea of S. philanthi RM-1-138 was established at a higher level in several metabolic compounds in the dark condition (11 and 32 antifungal compounds after incubation at 21°C and 28°C, respectively) than in the light condition (11 and 19 antifungal compounds after incubation at 21°C and 28°C, respectively). At 21°C, the dominant component was acetic acid (67.41% and 68.77% in light and dark conditions, respectively) while at 28°C, benzeneacetamide (43.58% in light) and propanamide (20.68% in the dark) were dominant. The results clearly demonstrated the significant influence of environmental factors on the production of antifungal metabolites of Streptomyces spp.

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“…Different previous studies have shown that S. phaeopurpureus 5125, S. salmonicida 5472, and S. fulvissimus could grow well in light conditions (Koyama et al 1975). Some strains of Streptomyces species such as S. viridosporus, S. lividans and S. coelicolor were reported to be sensitive to light, while others remained unaffected (S. ambofaciens and S. pilosus) (Imbert and Blondeau 1999). It has been demonstrated that light conditions in uenced the growth of Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Influences of Culture Media, Temperature and Light/Dark Conditions on Growth and Antifungal Activity of Streptomyces spp. Against Botrytis cinerea, in vitro and on Tomato Leaf

Boukaew

Yossan

Cheirsilp

et al. 2021

Preprint

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The influences of culture media, temperature, and light/dark conditions on growth and antifungal activity of the three strains Streptomyces spp. against Botrytis cinerea was studied. Results of in vitro study indicated that the GYM agar and incubated at 28°C exhibited good mycelial growth and a spore mass production of the three strains of Streptomyces spp. On the other hand, the PDA and incubation at 21°C were suitable for the mycelial growth of B. cinerea . Moreover, light/dark conditions had an effect on the growth of the two strains of S. philanthi . The strains RL-1-178 and RM-1-138 of S. philanthi grown in all media and incubation temperatures tested possessed antifungal activity against B. cinerea (100% inhibition) while S. mycarofaciens showed different results on PDA (83% inhibition) and GYM (88% inhibition) with the optimum incubation temperature at 21°C. Then, the antifungal compounds in culture filtrates produced by the three antagonistic strains against B. cinerea were tested on tomato leaf. They showed a significantly higher inhibitory effect on the symptoms of blight disease on tomato leaf compared with the control. The better protection efficacy against B. cinerea on tomato leaf was observed with the culture filtrates of S. philanthi RM-1-138 (82.89% and 0.33 cm 2 lesion areas symptoms). Moreover, the antifungal compounds in the culture filtrate of S. philanthi RM-1-138, identified by GC-MS, were greatly altered relative to concentration components under different temperatures and light/dark conditions tested. Our results clearly demonstrated that the environmental factors have an influence on antifungal activity of the three strains of Streptomyces spp.

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Effect of light on germinating spores of Streptomyces viridosporus

Cited by 4 publications

References 10 publications

Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

Time-Lapse Microscopy of Streptomyces coelicolor Growth and Sporulation

Impact of environmental factors on Streptomyces spp. metabolites against Botrytis cinerea

Influences of Culture Media, Temperature and Light/Dark Conditions on Growth and Antifungal Activity of Streptomyces spp. Against Botrytis cinerea, in vitro and on Tomato Leaf

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