In architectural spaces, the ambiance created by lighting plays a significant role in influencing people’s emotions, often relying on the use of color and light. However, previous research has primarily focused on urban architectural spaces, with relatively less attention given to rural environments, especially the interior spaces of rural accommodation. The objective of this study is to investigate the impact of colored lighting within rural accommodation interiors on people’s emotions and visual perceptions. Additionally, the study aims to establish an emotional dimension model to map the experimental results onto eight basic emotions, utilizing the PAD model for quantitative analysis. To achieve this goal, the researchers recruited 90 participants and divided them into six groups, each experiencing different combinations of indoor lighting scenes with varying colors. Participants evaluated their emotions and visual perceptions. The study results indicate that the combination of cool and warm white light with colored light significantly influences the arousal level of participants but has a relatively lower impact on pleasure levels, dominance and relaxation levels. In comparing the emotions experienced after exposure to cool and warm white light followed by colored light, warm white light elicited more positive emotions. Furthermore, colored light following cool white light diminished the sense of warmth in light, while yellow–blue light enhanced visual comfort and blue–red light increased attraction. Overall, the effect of colored lighting in rural accommodation interior spaces on improving negative emotions was relatively weak. Conversely, the combination of warm white light with blue–yellow or green–yellow light had a more significant effect on enhancing visual perceptions and reducing participants’ anxiety. This research provides valuable insights and references for designing the lighting ambiance in rural accommodation interiors and lighting design, with the potential to enhance the spatial experience of rural accommodations.