Effect of lon Protease Overexpression on Endotoxin Production and Stress Resistance in Bacillus thuringiensis

Barkad, Mouktar Abdi; Bayraktar, Aslı; Doruk, Tuğrul; Tunca, Sedef

doi:10.1007/s00284-021-02610-w

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…By regulating the amount of specific functional proteins, the proteases participate in transcriptional and post-translational pathways. In bacteria, intracellular proteolysis is carried out by ATP-dependent proteases that belong to the large AAA+ family proteins (ATPase associated with various cellular activities), such as Lon, FtsH, HslVU and the Clp family (ClpAP and ClpXP) [ 1 , 2 ]. Among them, FtsH is the only essential and membrane-anchored protease [ 3 ], and Lon is present in all of the kingdoms of life.…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, there are several studies that show that the overexpression of Lon protease can be either beneficial or detrimental depending on the organism [ 33 , 34 ]. In Streptomyces coelicolor , an additional copy of the Lon protease gene increases the rate of antibiotic synthesis [ 35 ], whereas in Bacillus thuringiensis , the overexpression of Lon leads to the degradation of activator proteins that are vital for motility, thereby limiting bacterial motility [ 2 ] ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival

Kirthika

Lloren

Jawalagatti

et al. 2023

IJMS

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Proteases are the group of enzymes that carry out proteolysis in all forms of life and play an essential role in cell survival. By acting on specific functional proteins, proteases affect the transcriptional and post-translational pathways in a cell. Lon, FtsH, HslVU and the Clp family are among the ATP-dependent proteases responsible for intracellular proteolysis in bacteria. In bacteria, Lon protease acts as a global regulator, governs an array of important functions such as DNA replication and repair, virulence factors, stress response and biofilm formation, among others. Moreover, Lon is involved in the regulation of bacterial metabolism and toxin–antitoxin systems. Hence, understanding the contribution and mechanisms of Lon as a global regulator in bacterial pathogenesis is crucial. In this review, we discuss the structure and substrate specificity of the bacterial Lon protease, as well as its ability to regulate bacterial pathogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival

Kirthika

Lloren

Jawalagatti

et al. 2023

IJMS

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Expression of Multiple Copies of the Lon Protease Gene Resulted in Increased Antibiotic Production, Osmotic and UV Stress Resistance in Streptomyces coelicolor A3(2)

Yilmaz,

Yaradir,

Tunca

2024

Curr Microbiol

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An overview of the production and use of Bacillus thuringiensis toxin

Li,

Chen,

Shi

et al. 2024

Open Life Sciences

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The widespread utilization of traditional chemical pesticides has given rise to numerous negative impacts, leading to a surge in interest in exploring environmentally friendly alternatives. Bacillus thuringiensis (Bt), a bacterium renowned for its insecticidal properties, produces Cry proteins during its lifecycle. These proteins have distinct advantages over traditional chemical pesticides, including higher environmental safety, broader insecticidal spectra, and lower pesticide residues. Consequently, the discovery and application of Bt hold immense significance in plant disease and pest management, as well as in plant protection. Currently, Bt preparations occupy a prominent position as the world’s largest and most widely used biopesticides. This article comprehensively reviews the fundamental aspects, insecticidal mechanisms, practical applications, and fermentation technologies related to Bt.

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Effect of lon Protease Overexpression on Endotoxin Production and Stress Resistance in Bacillus thuringiensis

Cited by 3 publications

References 47 publications

Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival

Structure, Substrate Specificity and Role of Lon Protease in Bacterial Pathogenesis and Survival

Expression of Multiple Copies of the Lon Protease Gene Resulted in Increased Antibiotic Production, Osmotic and UV Stress Resistance in Streptomyces coelicolor A3(2)

An overview of the production and use of Bacillus thuringiensis toxin

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