Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm

Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids

“…However, Lee et al [7] showed that culture potential of mouse spermatogonial stem cells gradually decreased during the first months storage in liquid nitrogen. Previous studies using Persian sturgeon and red seabream [1,2] also shown that fertilization rates of frozen-thawed sperm decreased significantly in relation to the storage duration in liquid nitrogen. Furthermore, Riesco and Robles [11] demonstrated that cryopreservation caused genetic and epigenetic changes in zebrafish genital ridges containing primordial germ cells.…”

Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)

“…In past experiments, 1:3 dilution ratio and 2-mL cryotube usually be used for marine fish sperm cryopreservation and obtained good results [18,22,28]. In this study, we improved the cryopreservation method from using 2-mL cryovial to 5-mL cryovial, the dilution ratio from 1:3 Table 2 The changes of postthaw sperm motion characteristics in summer flounder (Paralichthys dentatus).…”

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

“…A longer storage may affect sperm quality. A significant decline in motility of post-thawed sperm following long-term cryopreservation has been reported for African catfish (C. gariepinus; Rurangwa, Volckaert, Huyskens, Kime & Ollevier 2001), Japanese eel (Anguilla japonica; Tanaka Chen, Liu, Li, Xiao, Xu, Shi & Ma 2010). However, long-term storage of cryopreserved sperm did not cause a decrease in motility of post-thawed sperm in other studies.…”

“…However, failures in terms of sperm motility and viability among studies may be partly attributed to cryoinjury and/or oxidative damage to spermatozoa during cryopreservation, the latter, by generation of reactive oxygen species (Basavaraja & Hegde 2004). Oxidative damage during sperm cryopreservation has been known to induce lipid peroxidation, which affected spermatozoa structure and function and DNA damage (Watson 2000;Chen et al 2010;Li, Hulak, Koubek, Sulc, Dzyuba, Boryshpolets, Rodina, Gela, Manaskova-Postlerova, Peknicova & Linhart 2010). Further research about effect of oxidative stress during long-term storage of cryopreserved B. gonionotus spermatozoa on DNA damage should be explored.…”