Effect of Long-Term of He-Ne Laser Light Irradiation on Selected Physiological Processes of Triticale

Abstract

In agriculture, the bio-stimulating properties of laser light increase the yielding capacity of crop species. The experiment aimed to determine the pre-sowing effect of irradiation time with laser He-Ne red light of triticale grains (×Triticosecale Wittm. ex A.Camus) on germination and selected morphological and physiological parameters of seedlings and plants grown from them. The highest values of germination indexes were found for grains irradiated with laser for 3 h. In relation to the control, the elongation growth of seedlings was stimulated in grains irradiated with light for 3 h and inhibited for 24 h. The values of the fresh and dry mass of seedlings changed depending on the exposure time. He-Ne light did not significantly affect the degree of destabilization of seedling cell membranes. Biometric analysis of plants grown from irradiated grains showed different reactions of triticale organs to the irradiation time. Red light clearly stimulated the increase in the value of organ mass. Chlorophyll content in leaves was higher in plants grown from grains irradiated for 3 h. Photosynthetic activity did not change significantly relative to the control. The fluorescence emission indexes were mostly lower than in the control, which indicated a positive effect of the laser. In general, the red light of the laser stimulated the morphology and physiology of seedlings and plants, although, for some features, long exposure to red light caused a slight reduction effect.

He-Ne Laser Seed Treatment Improves the Nutraceutical Metabolic Pool of Sunflowers and Provides Better Tolerance Against Water Deficit

“…Plants grown from seeds exposed for 2 min to He-Ne laser treatment showed increased carotenoid content. This change is in accordance with Możdżeń et al (2020). Carotenoids are antioxidants and accessory pigments of photosynthesis.…”

“…In a previous study in triticale, the chlorophyll a level was almost identical in untreated and irradiated (He-Ne) plants. Możdżeń et al (2020) justified this outcome with the immediate transfer of excitation energy from antennae to the reaction center. In the current work, the elevated chlorophyll a in treated plants showed a different pattern of energy transfer in sunflower compared with triticale.…”

“…Treated seeds show better mechanical strength (Dziwulska-Hunek et al, 2021) with positive outcomes in a number of species including sunflower (Perveen et al, 2011;Afzal et al, 2019;Hasan et al, 2020). Different combinations of red and white light variably affect growth, photosynthetic pigments (He et al, 2020), and the phytochrome-mediated physiological responses of normal and stressed plants (Możdżeń et al, 2020). However, information regarding the effect of He-Ne laser seed treatment on the levels of nutraceuticals in sunflowers subjected to drought stress has not been reported.…”

Influence of Silver Nanoparticles, Laser Light and Electromagnetic Stimulation of Seeds on Germination Rate and Photosynthetic Parameters in Pumpkin (Cucurbita pepo L.) Leaves

“…It was the highest in the SII (14%) and SIII (4%) phases. In other studies on long-term laser beam irradiation (wavelength 632.8 nm), it was noticed that the photosynthetic activity did not differ significantly between the research objects [79]. According to research study by Li et al [80] regarding the effect of red (R), white (B) and mixed light (RB) on the photosynthesis efficiency in red pepper leaves, it was noticed that the leaves were thicker and the ability to transpose electrons in the process of photosynthesis and its speed increased by lights B and RB.…”

He–Ne laser accelerates seed germination by modulating growth hormones and reprogramming metabolism in brinjal

“…Further laser irradiation is also involved in increasing nitrogen level which leads to higher protein content to increase plant organs such as number of leaves and leaf area, number of branches, and umbels 47 , 48 . The increased leaf area, which is the reflection of laser rays enhanced cell division and elevated level of GA and subsequent growth during vegetative stage 49 – 51 and improved morphological as well as physiological features 52 . The laser-mediated elevation of GA3 associated with several physiological functions such as cell elongation, auxin, and sugar content and subsequently increased the plant height, number of branches, and flowers 53 , 54 .…”