Effect of long-term storage at −80 °C on the various lipid parameters in stored plasma samples

Devanapalli, Beena; Bermingham, Margaret; Mahajan, Deepika

doi:10.1016/s0009-8981(02)00137-7

Cited by 18 publications

(15 citation statements)

References 15 publications

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“…A similar study on various lipid parameters [5] showed a sharp decrease in total cholesterol, triglycerides, and lipoprotein(a) after 1 year at ¡80°C.…”

mentioning

confidence: 58%

Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Rouy

Ernens

Jeanty

et al. 2005

Analytical Biochemistry

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“…A similar study on various lipid parameters [5] showed a sharp decrease in total cholesterol, triglycerides, and lipoprotein(a) after 1 year at ¡80°C.…”

mentioning

confidence: 58%

Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Rouy

Ernens

Jeanty

et al. 2005

Analytical Biochemistry

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“…Longer-term stability of human specimens has been examined for storage at −20 °C [5][6][7][8][9], −25 °C [10], and −30 °C [11] in comparison to 4 °C and/or RT. Most reports showed a gradual decrease of LDH, ALT, CK, and an increase of the C3 test results in the frozen states over a period of 28-90 days.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of stability profiles of common chemistry analytes in serum stored at six graded temperatures

Shimizu

Ichihara

2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background Many reports address the stability of biochemical analytes in serum. However, studies covering a wide range of storage temperatures are unavailable. Using equipment enabling precise temperature control, we investigated the effect of six different storage temperatures on serum analytes. Methods Serum specimens from seven healthy volunteers were obtained and divided into multiple aliquots for storage at −30, −20, −10, 0, 4, and 25 °C. On days 1, 3, 7, 14, 28 and 56, the aliquots stored at each temperature were relocated to a deep freezer maintained at −80 °C. On day 60, all aliquots were measured collectively for 13 major chemistry analytes. Results (1) At 25 °C, alanine aminotransferase (ALT), creatine kinase (CK), aspartate aminotransferase (AST) and total bilirubin (TBil) were very unstable especially on day 7 and later. (2) At ≤4 °C, alkaline phosphatase (ALP), γ-glutamyltransferase (GGT), amylase (AMY), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), triglyceride (TG), TBil and complement component-4 (C4) were generally stable and were very stable at 25 °C until day 14. (3) Between −20 and 4 °C, especially at −10 °C, test results of ALT, AST and lactate dehydrogenase (LDH) showed prominent decreases, but their stability was greatly improved at −30 °C. (4) In contrast, the value of complement component-3 (C3) increased at ≥− 20 °C. (5) At −30 °C, test results of all analytes were generally very stable except for ALT and CK, which showed noticeable reductions in activity after 14 days. Conclusions This is the first study to assess the stability of serum analytes at six graded temperatures simultaneously. Each analyte has a unique stability pattern for a range of temperatures.

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“…Second, the term delivery group was relatively small and comprised women from another cohort with a different study protocol, in which samples were stored at −80 °C prior to analysis. Storage might lead to an underestimation of total cholesterol and triglycerides and an overestimation of HDL‐c . If this is true for the present data, the differences between the lipid levels of the sPTD and reference group would increase, but the conclusions would remain the same.…”

Section: Discussionmentioning

confidence: 57%

Cardiovascular biochemical risk factors among women with spontaneous preterm delivery

Heida

Kampman

Franx

et al. 2018

Intl J Gynecology & Obste

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Effect of long-term storage at −80 °C on the various lipid parameters in stored plasma samples

Cited by 18 publications

References 15 publications

Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Elucidation of stability profiles of common chemistry analytes in serum stored at six graded temperatures

Cardiovascular biochemical risk factors among women with spontaneous preterm delivery

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