Effect of low-intensity laser radiation of the red spectrum on some properties of erythrocytes in Wistar rats

Линькова, Н С; Gorshkova, O. P.; Shuvaeva, V. N.; Dvoretskii, D. P.

doi:10.1007/s10517-008-0020-y

Cited by 6 publications

(1 citation statement)

References 2 publications

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“…Actually LLL therapy does not induce thermal changes for that reason; it will not cause living cell damage [8]. The erythrocyte sedimentation rate (ESR) is one of the vital rheological considerations of human blood [9]which is frequently used to screen for presence of inflammation. It is referred to as the ESR because itmeasures the rate at which red blood cells (RBCs) separate from the liquid part of blood (plasma) and fall to the bottom of a test tube forming sediment.…”

Section: Introductionmentioning

confidence: 99%

Effect of laser on erythrocytes sedimentation rate and some hematological parameters

Shaker

Rasheed

Salman

2017

J. Fac. Med Baghdad

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Background: This study was conducted in pursuit of gaining an understand effects of the low level laser irradiation on whole blood is very essential way in revealing the mechanisms of the action of laser radiation on biological tissues. Objective: The purpose of this study is to investigate the in vitroeffect of laser radiation on some hematological parameters and erythrocyte sedimentation rate. Subject and Method: Blood samples were obtained from 30 healthy volunteers, each sample was divided into four new samples, one of them was considered as control while the other three were exposed to three different laser doses. Thewavelength of532nm was used for irradiation with 4mm diameter beam spot on blood samples, with power density of 796.17mW/cm2. The irradiation times were 1.8, 3.7and 6.2 secand so the different doses of irradiation 1.5,3 and 5 J/cm2, respectively. Results: The erythrocyte sedimentation rate was measured after laser irradiation and compared with un-irradiated control sample. The results of this study showed thaterythrocyte sedimentation rate value increased significantly with a dose of 1.5J/cm² but not with the othertwo doses' values in male persons but not in females. Moreover, the mean cell volume shows significant decrement post irradiation. Conclusion: Laser irradiation induces physical changes in red blood cell membrane permeability and blood viscosity and consequently alters erythrocyte sedimentation rate.

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Section: Introductionmentioning

confidence: 99%

Effect of laser on erythrocytes sedimentation rate and some hematological parameters

Shaker

Rasheed

Salman

2017

J. Fac. Med Baghdad

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The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation

et al. 2014

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The therapeutic effects of low-power laser radiation of different wavelengths and light doses are well known, but the biochemical mechanism of the interaction of laser light with living cells is not fully understood. We have investigated the effect of MLS (Multiwave Locked System) laser near-infrared irradiation on cell membrane structure, functional properties, and free radical generation using human red blood cells and breast cancer MCF-4 cells. The cells were irradiated with low-intensity MLS near-infrared (simultaneously 808 nm, continuous emission and 905 nm, pulse emission, pulse-wave frequency, 1,000 or 2,000 Hz) laser light at light doses from 0 to 15 J (average power density 212.5 mW/cm(2), spot size was 3.18 cm(2)) at 22 °C, the activity membrane bound acetylcholinesterase, cell stability, anti-oxidative activity, and free radical generation were the parameters used in characterizing the structural and functional changes of the cell. Near-infrared low-intensity laser radiation changed the acetylcholinesterase activity of the red blood cell membrane in a dose-dependent manner: There was a considerable increase of maximal enzymatic rate and Michaelis constant due to changes in the membrane structure. Integral parameters such as erythrocyte stability, membrane lipid peroxidation, or methemoglobin levels remained unchanged. Anti-oxidative capacity of the red blood cells increased after MLS laser irradiation. This irradiation induced a time-dependent increase in free radical generation in MCF-4 cells. Low-intensity near-infrared MLS laser radiation induces free radical generation and changes enzymatic and anti-oxidative activities of cellular components. Free radical generation may be the mechanism of the biomodulative effect of laser radiation.

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Erythrocyte sedimentation rate of human blood exposed to low-level laser

et al. 2016

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This study is designed to investigate in vitro low-level laser (LLL) effects on rheological parameter, erythrocyte sedimentation rate (ESR), of human blood. The interaction mechanism between LLL radiation and blood is unclear. Therefore, research addresses the effects of LLL irradiation on human blood and this is essential to understanding how laser radiation interacts with biological cells and tissues. The blood samples were collected through venipuncture into EDTA-containing tubes as an anticoagulant. Each sample was divided into two equal aliquots to be used as a non-irradiated sample (control) and an irradiated sample. The aliquot was subjected to doses of 36, 54, 72 and 90 J/cm(2) with wavelengths of 405, 589 and 780 nm, with a radiation source at a fixed power density of 30 mW/cm(2). The ESR and red blood cell count and volume are measured after laser irradiation and compared with the non-irradiated samples. The maximum reduction in ESR is observed with radiation dose 72 J/cm(2) delivered with a 405-nm wavelength laser beam. Moreover, no hemolysis is observed under these irradiation conditions. In a separate protocol, ESR of separated RBCs re-suspended in irradiated plasma (7.6 ± 2.3 mm/h) is found to be significantly lower (by 51 %) than their counterpart re-suspended in non-irradiated plasma (15.0 ± 3.7 mm/h). These results indicate that ESR reduction is mainly due to the effects of LLL on the plasma composition that ultimately affect whole blood ESR.

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Effect of low-intensity laser radiation of the red spectrum on some properties of erythrocytes in Wistar rats

Cited by 6 publications

References 2 publications

Effect of laser on erythrocytes sedimentation rate and some hematological parameters

Effect of laser on erythrocytes sedimentation rate and some hematological parameters

The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation

Erythrocyte sedimentation rate of human blood exposed to low-level laser

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