Effect of Low‐Intensity Pulsed Ultrasound on Bone Morphogenetic Protein 7–Induced Osteogenic Differentiation of Human Nonunion Tissue‐Derived Cells In Vitro

Koga, Takaaki; Lee, Sang Yang; Niikura, Takahiro; Koh, Akihiro; Dogaki, Yoshihiro; Okumachi, Etsuko; Akisue, Toshihiro; Kuroda, Ryosuke; Kurosaka, Masahiro

doi:10.7863/ultra.32.6.915

Cited by 17 publications

(25 citation statements)

References 28 publications

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“…The electronic search of the literature retrieved 1532 citations, but only 21 of them met the selection criteria 14 , 18 , 19 , 22 , 30 , 35 , 37 – 51 . Another three eligible papers 32 , 52 , 53 were obtained from the hand search of the references of the eligible studies and relevant review articles, yielding 24 eligible studies for the final analysis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Another three eligible papers 32 , 52 , 53 were obtained from the hand search of the references of the eligible studies and relevant review articles, yielding 24 eligible studies for the final analysis (Fig. 1 ) 14 , 18 , 19 , 22 , 30 , 32 , 35 , 37 – 53 .…”

Section: Resultsmentioning

confidence: 99%

“…In vitro investigations to evaluate osteogenic activity include measurements of a number of secreted substances (proteins) including: alkaline phosphatase (ALP), osteonectin, osteopontin, osteocalcin and bone sialoprotein. Alkaline phosphatase is a key protein secreted by osteoblasts in response to osteogenic activity and represents a marker of the earlier stage of osteoblast differentiation 18 . Osteonectin, osteopontin and osteocalcin are non-collagenous bone matrix proteins, abundant in bone tissue 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Osteonectin, osteopontin and osteocalcin are non-collagenous bone matrix proteins, abundant in bone tissue 19 . They are thought to be of great importance in bone development, growth, turnover and fracture repair; along with osterix, as essential factor for osteoblast differentiation and bone formation, they represent markers of the later stage of differentiation 18 – 20 . Bone Sialoprotein, an extracellular matrix protein secreted by osteoblastic cells, has also been reported to modulate osteoblast differentiation and mineralization 21 .…”

Section: Introductionmentioning

confidence: 99%

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Biological and molecular profile of fracture non‐union tissue: current insights

Panteli

Pountos

Jones

et al. 2015

J Cellular Molecular Medi

104

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Delayed bone healing and non-union occur in approximately 10% of long bone fractures. Despite intense investigations and progress in understanding the processes governing bone healing, the specific pathophysiological characteristics of the local microenvironment leading to non-union remain obscure. The clinical findings and radiographic features remain the two important landmarks of diagnosing non-unions and even when the diagnosis is established there is debate on the ideal timing and mode of intervention. In an attempt to understand better the pathophysiological processes involved in the development of fracture non-union, a number of studies have endeavoured to investigate the biological profile of tissue obtained from the non-union site and analyse any differences or similarities of tissue obtained from different types of non-unions. In the herein study, we present the existing evidence of the biological and molecular profile of fracture non-union tissue.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological and molecular profile of fracture non‐union tissue: current insights

Panteli

Pountos

Jones

et al. 2015

J Cellular Molecular Medi

104

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“…Studies have revealed that fracture healing is a complicated and consecutive process (7,8). At bone nonunion, hematoma and tissues between the fracture ends gradually develop into tissues between the fracture ends of bone nonunion (9). Tissues and hematoma join the fracture ends and in vitro its reaction is similar to that of bone mesenchymal stem cells.…”

Section: Introductionmentioning

confidence: 99%

Combined treatment with extracorporeal shock‑wave therapy and bone marrow mesenchymal stem cell transplantation improves bone repair in a rabbit model of bone nonunion

Fan

Huang

et al. 2017

Mol Med Report

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The aim of the present study was to analyze whether extracorporeal shock‑wave therapy (ESWT) combined with bone marrow mesenchymal stem cell (BMMSC) transplantation improves bone repair in a rabbit bone nonunion model. ESWT combined with BMMSC effectively enhanced mechanical strength, fracture stiffness and histological scores, and increased alkaline phosphatase activity, and osteopontin, runt related transcription factor 2 and collagen type I α1 chain protein expression levels in a rabbit bone nonunion model. In addition, ESWT combined with BMMSC effectively enhanced insulin‑like growth factor 1 and vascular endothelial growth factor contents, promoted transforming growth factor‑β (TGF‑β) contents, and induced the growth factors, bone morphogenetic protein (BMP)‑2, BMP‑4 and purinergic receptor P2X7 (P2X7) protein expression in the rabbit bone nonunion model. Thus, the present study demonstrated that ESWT combined with BMMSC transplantation improves bone repair in a rabbit bone nonunion model via the BMPs and P2X7 signaling pathways.

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