Effect of <I>STARD3</I> gene polymorphism on carcass traits and fatty acid composition in Japanese Black cattle

Abstract

In this study, we focused on the STARD3 gene which is involved in cholesterol distribution and homeostasis in cells, and investigated the effects of the STARD3 gene polymorphism on carcass traits and fatty acid composition. We searched for polymorphisms in the STARD3 gene by whole-genome resequencing and selected a missense S396L from 280 detected polymorphisms. We genotyped S396L polymorphism for Japanese Black cattle population (n = 441) and then analyzed the effects on carcass traits and fatty acid composition. As a result, the polymorphism showed strong association with fatty acid composition (C14:0, C16:0, C18:1, SFA and MUFA) (p<0.0001). Although further research is warranted to clarify whether S396L directly impacts fatty acid composition, it is obvious that there is a novel QTL for fatty acid composition on BTA19 in the Japanese Black cattle. In conclusion, these results contribute to the identification of a novel causative mutation on BTA19 for fatty acid composition and development of a useful DNA marker for improving the beef quality of Japanese Black cattle.

Application of linear and machine learning models to genomic prediction of fatty acid composition in Japanese Black cattle

“…Thus, these polymorphisms are considered the most likely candidates to affect fatty acid composition. In addition, SREBP1 , STARD3 , and GH polymorphisms located near FASN polymorphisms are also associated with fatty acid composition (Hoashi et al, 2007; Kigoshi et al, 2019; Matsuhashi et al, 2011). For GC‐C18:1 and GC‐MUFA, KAML chose seven SNP markers as pseudo QTNs, of which location information was described in Table S1.…”

Effect of Five Polymorphisms on Percentage of Oleic Acid in Beef and Investigation of Linkage Disequilibrium to Confirm the Locations of Quantitative Trait Loci on BTA19 in Japanese Black Cattle

“…The current study was focused on five polymorphisms ( Table 2 ). Among them, SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, and FASN g.16024 A > G were genotyped in the Hyogo population in previous studies [ 8 , 20 ]. Additionally, FASN g.16024 A > G was also previously genotyped in the Gifu population [ 5 ].…”

“…The genotyping results were obtained for each animal from these previous studies. Additionally, genotyped FASN g.841 G > C was obtained in both populations and SREBP1 c.1065 + 83 and STARD3 c.1187 C > T in the Gifu population using the method described in the previous studies [ 5 , 7 , 8 ]. GH c.379 C > G was additionally genotyped using restriction fragment length polymorphism of fragments amplified by polymerase chain reaction and TaqMan hydrolysis probes in the Hyogo and Gifu populations.…”

“…Among them, two single-nucleotide polymorphisms (SNPs) within the FASN gene (g.841 G > C and g.16024 A > G), which are located on about 51.4 Mbp, are well known for being polymorphisms that are likely responsible for fatty acid composition because of associations between the polymorphisms and fatty acid composition in various cattle populations [ 3 , 4 , 5 , 6 ]. Additionally, three polymorphisms within the SREBP1 , STARD3 , and GH genes have also been reported to be significantly associated with fatty acid composition [ 7 , 8 , 9 ]. These three polymorphisms, SREBP1 c.1065 + 83, STARD3 c.1187 C > T, and GH c.379 C > G, were located on about 35.2, 40.7, and 48.6 Mbp on BTA19, respectively.…”