Effect of luteinising-hormone<i>in vivo</i>on ovarian cell subpopulations of newly-hatched chicks

González-Morán, Genoveva; Calderon, Silvia N.

doi:10.1080/713654960

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…The present findings and previous reports show that interstitial cells of the left ovary from newly hatched chick and theca cells are steroidogenic and target cells of estrogen (Narbaitz & De Robertis 1968, Davidson et al 1979, Gonza´lez-Mora´n & Calderon 2000.…”

Section: (B) Ovarian Cortex -Germinal Epithelium Cells (Ge) and Clustsupporting

confidence: 86%

“…These interstitial cells are clearly identified in the left ovary of the newly hatched chick, forming cell clusters with abundant cytoplasmatic lipid droplets, and a positive reaction for D5-3bHSD activity has been detected in these cell groups (Gonza´lez-Mora´n & Calderon 2000). Samar et al (1983) identified interstitial cells in the right medullary ovary of the chick embryo, with the same characteristics and positive reaction for D5-3bHSD as the left ovary.…”

Section: (B) Ovarian Cortex -Germinal Epithelium Cells (Ge) and Clustmentioning

confidence: 97%

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Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Morán

2005

J Mol Hist

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The aim of this study was to detect the presence of estrogen receptor alpha in different cell subpopulations of the growing left and regressing right ovaries from newly hatched chicks, by immunohistochemical methods, using an indirect immunoperoxidase technique. Newly hatched chicks were killed by decapitation and the growing left and regressing right ovaries were removed and processed for histological and immunohistochemical studies. The percentage of estrogen receptor alpha (ERalpha) immunostained cells and the staining intensity were determined. Histological results demonstrated that the right ovary lacks cortex and is constituted by the medullary region, whereas the left ovary is composed by cortical and medullary compartments. The medullary region is similar in both ovaries and presents the same elements. Immunohistochemical analysis revealed ERalpha-positive cells in the left ovary, located in the nucleus of all cell subpopulations; in contrast, in the right ovary, only epithelial cells of the lacunar channels depicted estrogen receptors. The present findings indicate a cell-specific localization of ERalpha in left and right ovaries of newly hatched chick.

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Section: (B) Ovarian Cortex -Germinal Epithelium Cells (Ge) and Clustsupporting

confidence: 86%

Section: (B) Ovarian Cortex -Germinal Epithelium Cells (Ge) and Clustmentioning

confidence: 97%

Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Morán

2005

J Mol Hist

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“…It is noteworthy to mention that our observations indicate that the total volume of interstitial cells is the dominant component in the right ovary (55%) of 4‐week‐old chickens; cell hypertrophy is mainly responsible for the increase of total volume of interstitial cells. Previous works have detected a positive reaction for Δ5‐3βHSD activity in interstitial cells in the left and right ovaries of chicken embryo and newly hatched chicken (Narbaitz and Kolodny,1964; Scheib and Haffen,1967; Samar et al,1983; González‐Morán and Calderon,2000), confirming the steroidogenic function of these cells in both ovaries. Other authors have indicated that the left and right ovaries of the chicken embryo secrete steroid hormones and respond to gonadotropins with an increase in cyclic AMP concentration and in 17β‐estradiol and testosterone production (Teng and Teng,1977,1979; Teng et al,1982); both left and right medulla express aromatase and produce estrogens (Nakabayashi et al,1998; Endo et al,2007), and ERα is expressed symmetrically in both sides of the medulla (Andrews et al,1997; Smith et al,1997), but, in a previous work, we demonstrated a cell‐specific localization of ERα in the cortex and medulla of left and right ovaries (González‐Morán,2005).…”

Section: Discussionmentioning

confidence: 53%

“…The number of interstitial cells per left and right ovaries, volume of the individual interstitial cell, and volume of the nucleus and cytoplasm of interstitial cells were determined according to González‐Morán (1997) and González‐Morán and Calderon (2000).…”

Section: Methodsmentioning

confidence: 99%

Histological and Stereological Changes in Growing and Regressing Chicken Ovaries During Development

González-Morán

2011

The Anatomical Record

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The aim of this study was to evaluate the sequence of the histological and stereological changes that occur in diverse components of left growing and right regressing ovaries of Gallus domesticus as well as the variations in the number and size of the different cell subpopulations from 8-day-old chicken embryo to 4-week-old chickens. Results indicate a bilateral asymmetry in favor of the left ovary at all ages studied. The left ovary shows cortex and medulla, but the right ovary has no cortex, only a medulla. Stereological results show in the left ovary an increase in total volume of all components of the ovarian medulla with advancing development. The right ovary follows a similar pattern until the age of 1-day-old chicken, to regress afterward, thereby, reducing the total volume of blood vessels, lacunar channels, and interstitium, but increasing the total volume of interstitial cells as development progresses; consequently, the total volume of interstitial cells is the dominant component in the right ovary of 4-week-old chickens. Results also reveal that the increase in total volume of interstitial cells in the left ovary at all ages and in the right ovary from 8-day-old chicken embryo to 1-day-old chicken is due to hyperplasia and cell hypertrophy of interstitial cells; hence, in the right ovary, cell hypertrophy is the main cause for the increase in the total volume of interstitial cells from 1-week-old to 4-week-old chickens. Anat Rec, 294:893-904, 2011. V V C 2011 Wiley-Liss, Inc.Key words: histology; stereology; left ovary; right ovary; chickenIn vertebrates, the reproductive system arises as bilateral anlagen, and consequently paired genital organs are commonly found in the adult. In birds, the female reproductive system is unique. Although paired anlagen appear, only the left genital primordia develop further to functional organs, except in birds of prey (Jacob and Bakst, 2007).In the chick, formation of the ovary differs between the right and left sides. In the left ovary, whose development proceeds normally, cortical cords (secondary cords) extend inward from the gonadal epithelium. From 7 days of incubation onward, the cords develop to form a cortex, whereas the medulla is composed of epithelium derived from medullary cords (primary cords) and their enlarged inner spaces (medullary lacunae). In contrast, the right ovary stops further development from 7 days onward. This is due to the absence of secondary cord formation; the right ovary has only a medulla composed of the epithelium of the medullary cord and enlarged lacunae. Eventually, the right ovary becomes smaller than the left (Romanoff, 1960;Stahl and Carlon, 1973;Ebensperger et al

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“…It was hypothesized that these responses were due to a paracrine (local) mechanism versus systemic endocrine feedback. Additionally, medullary cells of newly hatched chickens respond to equine LH by increasing their size and 3b-HSD activity, but not by cellular proliferation (Gonzalez-Moran and Calderon, 2000). In vitro dispersed cell cultures of left embryonic chicken ovary, treated with FSH, increased cell proliferation in later stages of incubation and estrogen and testosterone synthesis in all stages of incubation studied (Pedernera et al, 1999).…”

Section: Discussionmentioning

confidence: 93%

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Moore

Uribe-Aranzábal

Boggs

et al. 2007

Journal of Morphology

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American alligator (Alligator mississippiensis) ovary development is incomplete at hatching. During the months following hatching, the cortical processes of oogenesis started in ovo continues and folliculogenesis is initiated. Additionally, the medullary region of the gonad undergoes dramatic restructuring. We describe alligator ovarian histology at hatching, 1 week, 1 month, and 3 months of age in order to characterize the timing of morphological development and compare these findings to chicken ovary development. At hatching, the ovarian cortex presents a germinal epithelium containing oogonia and a few primary oocytes irregularly scattered between somatic epithelial cells. The hatchling medulla shows fragmentation indicative of the formation of lacunae. By 1 week of age, oocytes form growing nests and show increased interactions with somatic cells, indicative of the initiation of folliculogenesis. Medullary lacunae increase in diameter and contain secretory material in their lumen. At 1 month, nest sizes and lacunar diameters continue to enlarge. Pachytene oocytes surrounded by somatic cells are more frequent. Trabeculae composed of dense irregular connective tissue divide cortical nests. Three months after hatching oocytes in meiotic stages of prophase I up to diplotene are present. The ovary displays many enlarged follicles with oocytes in diplotene arrest, thecal layers, lampbrush chromosomes, and complete layers of follicular cells. The medulla is an elaborated complex of vascularized lacunae underlying the cortex and often containing discrete lymphoid aggregates. While the general morphology of the alligator ovary is similar to that of the chicken ovary, the progression of oogenesis and folliculogenesis around hatching is notably slower in alligators. Diplotene oocytes are observed at hatching in chickens, but not until 3 months in alligators. Folliculogenesis is completed at 3 weeks in chickens whereas it is still progressing at 3 months in alligators.

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Effect of luteinising-hormonein vivoon ovarian cell subpopulations of newly-hatched chicks

Cited by 11 publications

References 18 publications

Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Histological and Stereological Changes in Growing and Regressing Chicken Ovaries During Development

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

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