Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Morán, María Genoveva González

doi:10.1007/s10735-004-6165-8

Cited by 14 publications

(4 citation statements)

References 35 publications

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“…The significance of asymmetric expression of ER and other embryonic stem cell markers in chicken embryos is still unknown. However, the germinal epithelial cells were reported to be estrogen target cells acting via estrogen receptor (Morán, 2005). From our pervious study, we found that in ovo exposure of phytoestrogen to male quail embryo caused thickening of the cortex of the left male gonad .…”

Section: Discussionmentioning

confidence: 82%

Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

2016

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INTARAPAT, S.; SAILASUTA, A. & SATAYALAI, O.Immunohistochemical localization of estrogen receptor in the embryonic gonad of male quail embryo during gonadal differentiation. Int. J. Morphol., 34(2):575-579, 2016. SUMMARY:In birds, male embryo the gonads develop bilateral testes, in which both left and right sides produce functional spermatozoa, whereas female embryo, only the left gonad develops into a functional ovary. Estrogen plays a key role in avian sex determination in both sexes by binding to the estrogen receptor (ER). Surprisingly, chicken estrogen receptor (cER) mRNA is expressed in both sexes; moreover; its expression is only expressed in the left male gonad. The present study aimed to localize ER protein in the left gonad of male quail embryo using immunohistochemistry. The 8-day-old male quail embryos whose embryonic sex distinguished by gonadal morphology were studied. Histology of the left male gonad displayed thin cortex containing 1 to 2 layers of the germinal epithelium, while testicular cords were observed in the medulla. ER-immunoreactive cells were only found in the germinal epithelium but not in the medulla. Localization of ER was detected in the nucleus and cytoplasm of the germinal epithelial cells. The number of ER-immunoreactive cells counted in upper, lateral, and lower regions of the germinal epithelium was 18.20±1.892, 17.60±1.887, and 16.20±1.290, respectively. This study shows the first evidence for expression of ER protein in the left male gonad of the avian embryo, indicating that ER plays a role in avian gonadal sex differentiation.

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Section: Discussionmentioning

confidence: 82%

Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

2016

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“…5 A). ERα is the nuclear receptor that is essential for normal ovarian differentiation ( 34 ). LIM homeobox protein 9 (LHX9) is a transcription factor critical for gonad development ( 35 ).…”

Section: Resultsmentioning

confidence: 99%

A PITX2–HTR1B pathway regulates the asymmetric development of female gonads in chickens

et al. 2023

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All female vertebrates develop a pair of ovaries except for birds, in which only the left gonad develops into an ovary whereas the right gonad regresses. Previous studies found that the transcription factor PITX2 (Paired Like Homeodomain 2), a key mediator for left/right morphogenesis in vertebrates, was also implicated in asymmetric gonadal development in chicken. In this study, we systematically screened and validated the signalings that could be targeted by Pitx2 to control unilateral gonad development. Integrated ChIP-seq and RNA-seq analyses indicated that Pitx2 directly binds to the promoters of genes encoding neurotransmitter receptors and leads to left-biased expression of both serotonin and dopamine receptors. Forcibly activating the serotonin receptor (HTR1B: 5-Hydroxytryptamine Receptor 1B) signaling could induce ovarian gene expression and cell proliferation to partially rescue the degeneration of the right gonads. In contrast, inhibiting the serotonin signaling could block the development of left gonad. These findings reveal a PITX2-HTR1B genetic pathway that guides the left-specific ovarian growth in chicken. We also provided new evidence showing neurotransmitters stimulate the growth of non-neuronal cells during the early development of reproductive organs well before innervation.

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“…This implied that in chicken SOX9 could not activate AMH as in mammals, consistent with the expression profiles of SOX9 and AMH reported by different researchers [Kent et al, 1996;Oreal et al, 1998;Shimada, 2002], as they found SOX9 expression appears in male gonads 1 day after the onset of AMH expression. Estrogen regulates gene expression, female sexual differentiation and female secondary sexual characteristics by binding to and activating the nuclear estrogen receptors [Morán, 2005], i.e. ER ␣ and ER ␤ , which belong to the nuclear receptor superfamily of ligand-dependent transcription factors [Mangelsdorf et al, 1995;Di Croce et al, 1999].…”

Section: Control Gonadmentioning

confidence: 99%

SMARCE1 Promotes Chicken Embryonic Gonad Development by Regulating ERα and AR Expression

Gong

Yang²,

Liu³

et al. 2012

Sex Dev

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SMARCE1 is one of some differentially expressed genes screened from a subtracted cDNA library between females and males during the period of sex differentiation. To understand the potential role of SMARCE1 in avian sex determination and differentiation, over-expression of SMARCE1 was performed in chicken embryos using the RCASBP.B retrovirus. Results showed that SMARCE1 expression was up-regulated in the infected embryonic gonads at the investigated stages (E6.5–E12.5) assessed by quantitative real-time RT-PCR, whole mount in situ hybridization and immunohistochemistry. With the over-expression of SMARCE1, CYP19A1, FOXL2, ERα, and SOX9 expression was significantly up-regulated while AR expression was significantly decreased in the male and/or female chicken gonad. Nevertheless, DMRT1 and AMH expression was not changed after the over-expression of SMARCE1. It is proposed that it might be via the SMARCE1-FOXL2 pathway that CYP19A1 expression was activated, and DMRT1 expression may be independent of the SMARCE1-SOX9 pathway. Meanwhile, the SMARCE1-AR pathway might be antagonized by up-regulated expression of ERα via estrogen-ER/androgen-AR signaling. The results of HE staining showed that the ovarian cortex was thickened and both testis seminiferous cord and interstitial cells were increased with the over-expression of SMARCE1. In conclusion, SMARCE1 can promote chicken embryonic gonad development by regulating the ERα and AR expression.

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Immunohistochemical detection of estrogen receptor alpha in the growing and regressing ovaries of newly hatched chicks

Cited by 14 publications

References 35 publications

Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

A PITX2–HTR1B pathway regulates the asymmetric development of female gonads in chickens

<b><i>SMARCE1</i></b> Promotes Chicken Embryonic<b> </b>Gonad Development by Regulating<b><i> ER</i></b>α and <b><i>AR </i></b>Expression

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