Effect of macrophage depletion by liposomes containing dichloromethylene-diphosphonate on endotoxin-induced uveitis

Immunoregulatory Role of Ocular Macrophages: The Macrophages Produce RANTES to Suppress Experimental Autoimmune Uveitis

“…It is well established that macrophage-derived cytokines/chemokines contribute to tissue damage during severe experimental autoimmune diseases (11,12). Since cytokines and chemokines appear to be crucial for ocular inflammation (16), we postulated that ocular-infiltrating macrophages actually produced the cytokines and chemokines that mediated EAU.…”

“…In uveoretinitis, macrophages are involved in the phagocytosis of rod outer segments (8 -10). Depletion of macrophages in the uveitis model in rats reduced the disease score; therefore, activated macrophages are major effectors of tissue damage in uveitis (11)(12)(13). Macrophages also are considered to be potent APCs during the induction of inflammatory autoimmune diseases (14,15).…”

Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

“…Severity was graded from 0 to 4 by an observer blind to treatment history as described previously (22) . The clinical grading was defined as follows: Grade 0 was defined as no obvious inflammatory response, Grade 1 as discrete dilation of the iris and conjunctival vessels, Grade 2 as moderate dilation of the iris and conjunctival vessels with moderate flare in the anterior chamber, Grade 3 as intense iridal hyperemia with intense flare in the anterior chamber, and Grade 4 as the clinical signs of Grade 3 plus fibrinous exudation and miosis.…”

Ocular transfer of retinal glial cells transduced ex vivo with adenovirus expressing viral IL-10 or CTLA4-Ig inhibits experimental autoimmune uveoretinitis

“…67 Briefly, sections were incubated with mouse monoclonal antibody anti-ED1 (Serotec, Oxford, UK, diluted 1:50), or with rabbit polyclonal anti-GFAP (Dakopatts, Trappes, France, diluted 1:100), then incubated, respectively, with Texas red-conjugated donkey anti-mouse IgG or with Texas red-conjugated …”

“…9 Immunoreactivity was also assessed by measuring the 3 H-thymidine incorporation by Ag-stimulated T cells from inguinal lymph nodes as previously described. 67 Finally, in some experiments, the antibody responses directed against S-Ag and adenovirus were analyzed. 66,68 Analysis of vIL-10 and CTLA4 expression…”