Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

Mancilla, Rodrigo A.; Canessa, Paulo; Manubens, Augusto; Vicuña, Rafael

doi:10.1016/j.fgb.2010.04.003

Cited by 21 publications

(15 citation statements)

References 42 publications

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“…Also, heterotrophic bacteria could simply use the protein laccase as substrate [48], which might be another reason for the decrease in laccase activity in the real wastewater. It is noteworthy that the compounds which are usually used in the defined WRF media to induce laccase activity, such as Cu 2+ [49], Mn 2+ [50] or veratryl alcohol [51], were not used in this study. Although only laccase was assayed as the fungal enzyme in this study, the involvement of other enzymes like MnP should not be neglected.…”

Section: Discussionmentioning

confidence: 99%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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Citation (APA)Zahmatkesh, M., Spanjers, H., & van Lier, J. B. (2017). A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum. Environmental Technology, 1-11. https://doi.org/10.1080/09593330.2017.1347718 Important noteTo cite this publication, please use the final published version (if applicable). Please check the document version above. CopyrightOther than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policyPlease contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. In this study, we tested a new approach to facilitate the application of white rot fungi (WRF) under non-sterile conditions, by introducing grain sorghum as carrier and sole carbon and nutrient source for WRF. To this end, Trametes versicolor was immobilized on sorghum, and its ability to remove humic acid (HA) from synthetic and real industrial wastewater was studied. HA removal was measured as colour reduction and also analysed via size exclusion chromatography (SEC). Under sterile conditions, 80% colour removal was achieved for both synthetic and real wastewater using immobilized WRF on sorghum, without adding any additional carbon or nutrient sources. Under non-sterile conditions, immobilized fungi could again remove 80% of the colour and reached a maximum of 40 U/L laccase activity. In contrast, non-immobilized fungi cultivated in non-sterile wastewater supplemented with additional nutrients, reached only 10% decolourization and maximum 5 U/L laccase activity. SEC analysis showed that bioremoval of HA by WRF was associated with degradation of HA. Finally, immobilized fungi were used to treat real wastewater, under non-sterile conditions, in a sequential batch order without renewing the immobilized fungi. Four batch feedings were conducted and 80%, 70%, 50% and 40% colour removal was achieved for each batch, respectively, over a total incubation period of 19 days.

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Section: Discussionmentioning

confidence: 99%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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“…However, these mutants were not defective in mnp3 and vp1 transcription, although mnp2 was significantly inactivated by mutations in wtr1 (Fig. Secretory regulation of MnP was previously suggested in the selective white-rot fungus Ceriporiopsis subvermispora (Mancilla et al, 2010). Salame et al (2013) showed that MnP2 was not predominantly produced on GP amended with 27 mM MnSO 4 , and targeted disruption of mnp2 decreased the Mn 21 -mediated Orange II decolourization capacity only slightly, leading us to conclude that the transcriptional inactivation of mnp2 caused by mutations in wtr1 may not be responsible for the loss of guaiacol oxidation activity.…”

Section: Discussionmentioning

confidence: 77%

Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white‐rot agaricomycete Pleurotus ostreatus

Nakazawa

Izuno

Kodera

et al. 2017

Environmental Microbiology

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White-rot fungi play an important role in the global carbon cycle because they are the species that almost exclusively biodegrade wood lignin in nature. Lignin peroxidases (LiPs), manganese peroxidases (MnPs) and versatile peroxidases (VPs) are considered key players in the ligninolytic system. Apart from LiPs, MnPs and VPs, however, only few other factors involved in the ligninolytic system have been investigated using molecular genetics, implying the existence of unidentified elements. By combining classical genetic techniques with next-generation sequencing technology, they successfully showed an efficient forward genetics approach to identify mutations causing defects in the ligninolytic system of the white-rot fungus Pleurotus ostreatus. In this study, they identified two genes - chd1 and wtr1 - mutations in which cause an almost complete loss of Mn -dependent peroxidase activity. The chd1 gene encodes a putative chromatin modifier, and wtr1 encodes an agaricomycete-specific protein with a putative DNA-binding domain. The chd1-1 mutation and targeted disruption of wtr1 hamper the ability of P. ostreatus to biodegrade wood lignin. Examination of the effects of the aforementioned mutation and disruption on the expression of certain MnP/VP genes suggests that a complex mechanism underlies the ligninolytic system in P. ostreatus.

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“…The addition of metal cations to cultivation media, in particular Mn 2+ , Cu 2+ and Fe 2+ , might induce laccase and peroxidase production since metal cations represent the main cofactors of these enzymes [41]. Indeed, the use of ethanol and aromatic compounds was previously reported to stimulate laccase activity [42].…”

Section: Resultsmentioning

confidence: 99%

A valuable peroxidase activity from the novel species Nonomuraea gerenzanensis growing on alkali lignin

Casciello

Tonin

Berini

et al. 2017

Biotechnology Reports

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Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

Cited by 21 publications

References 42 publications

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white‐rot agaricomycete Pleurotus ostreatus

A valuable peroxidase activity from the novel species Nonomuraea gerenzanensis growing on alkali lignin

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