Effect of media and media pH on in vitro propagation of ‘Nemaguard’ peach rootstock

Reeves, D. W.; Horton, B. D.; Couvillon, G. A.

doi:10.1016/0304-4238(83)90125-5

Cited by 16 publications

(8 citation statements)

References 8 publications

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“…While in the case of covering for more that two weeks, high humidity caused the appearance of fungi on soil surface and plant stems. Following these steps of vegetative micropropagation agrees with what has been found by many researches in fruit plants which were moved to open air field like apples (Snir and Erez, 1980), peaches (Reeves et al, 1983), walnut (MC granahan et al, 1988), and chestnut (Preece and Sutter, 1991;Hameed, 1994;Awad, 1995;Trigiano and Gray, 1996;KhairAllah, 1997 andGhazal, 1997). It is essential to raise transplanting success rate to the soil to…”

Section: Results and Discussion Initiation Stagesupporting

confidence: 84%

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

M.S.Duhoky¹

2010

Mesopotamia Journal of Agriculture

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The present study was carried out to study the micropropagation of Gardenia (Gardenia jasminoides) by using single nodes excised from soft cuttings using half strength MS salts, 30gl -1 sucrose, 7gl -1 Agar and different concentrations of plant growth regulators in culture medium. Results of the experiment at initiation stage revealed that the culture of single nodes of Gardenia on a medium containing 5mgl -1 BA gave the highest number of shoots (2.2 shoots/explant). Concerning the interaction, a nutrient medium containing (2 mgl -1 BA+ 0.4 mgl -1 IAA) gave the highest values of average number of shoots and leaves and length of new shoots (1.6 shoots/explant, 2 leaves/explant, 1.6cm respectively). At vegetative multiplication stage, the results showed that the medium supplemented by 2 mgl -1 BA gave the highest values of average number of shoots and leaves and length of new shoots (2.20 shoots/explant, 3.20 leaves/explant, 1.80cm respectively), whereas the medium supplemented by 0.3 mgl -1 IAA gave the highest number of shoots and length of new shoots. In order to promote the shoots produced at vegetative multiplication stage to root, it was noticed that the treatment of 8mgl -1 NAA gave the highest percentage of rooting (90%) and the medium supplemented by 12mgl -1 IAA gave (100%) rooting. Plantlets obtained were transferred to pots and acclimatized with 95 % success.

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Section: Results and Discussion Initiation Stagesupporting

confidence: 84%

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

M.S.Duhoky¹

2010

Mesopotamia Journal of Agriculture

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“…Culture medium pH affects nutrient availability and uptake (Minocha 1987) and has been shown to influence a number of plant developmental processes in vitro, including organogenesis (Zhang & Stoltz 1989), floral differentiation (Cousson et al 1989), micropropagation rate (Reeves et al 1983), secondary product formation (Hagimori et al 1983), cell division (Basu et al 1988), adventitious rooting (Williams et al 1985), somatic embryogenesis (Smith & Krikorian 1990a), xylogenesis (Khan et al 1986), and microspore culture (Barrow 1986). In some studies, very narrow pH optima (0.2 pH units) have been observed (Nesius & Fletcher 1973).…”

Section: Introductionmentioning

confidence: 99%

Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

1991

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“…reports on in vitro propagation of peach (Prunus persica (L.) Batsch) have been limited in number and have either: (a) excluded rooting [2,12,16]; (b) described limited rooting [17]; (c) described propagation of only a few cultivars [8,9,13,17]; or (d) used juvenile plant material [1,10,19]. Since peach cultivars can vary in their response to a specific culture medium [2], their requirements for each stage of micropropagation may differ.…”

Section: Introductionmentioning

confidence: 99%

Factors influencing in vitro multiplication and rooting of peach cultivars

Hammerschlag

Bauchan²,

Scorza

1987

Plant Cell Tiss Organ Cult

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Success at propagating peach (Prunus persica (L.) Batsch) scion cultivars in vitro has been limited. This study describes factors influencing in vitro multiplication and rooting of 8 peach scion cultivars and one rootstock, as well as acclimatization and genetic stability of these cultivars. Shoot multiplication was best when 8.8/~M 6-benzylamino purine (BA) was added to the shoot proliferation medium. Maximum rooting occurred when shoots were placed on I/2-strength Murashige and Skoog (MS) medium, stored in the dark at 4 °C for 35 to 40 days and then incubated on rooting medium in the dark at 26 °C for 14 days. All cultivars exposed to l/2-strength MS medium supplemented with 28.5/~M of either indoleacetic acid (IAA), indolebutyric acid (IBA) or ct-napthaleneacetic acid (NAA) rooted best on NAA medium. A 5-fold reduction in NAA concentration to 5.8/~M, the use of IAA plus phenolic rooting cofactors, and length of time shoots were in vitro prior to rooting each increased the percentage of rooting for most cultivars. No plant loss occurred during acclimatization. Cytogenetic analysis of micropropagated plants indicated that all plants were diploid, 2n = 2x = 16. Examination of the performance of in vitro propagated plants under field conditions is now in progress.

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Effect of media and media pH on in vitro propagation of ‘Nemaguard’ peach rootstock

Cited by 16 publications

References 8 publications

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

Factors influencing in vitro multiplication and rooting of peach cultivars

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