MICROPROPAGATION OF GARDENIA Gardenia jasminoides

M.S.Duhoky, Mosleh

doi:10.33899/magrj.2010.27961

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…Concerning the main effect of BA concentrations, there is a direct proportional relationship between the mean number of shoots formed per propagule and BA levels; whereas, the highest level of BA (0.250 mg/l); gave rise to the highest mean value of number of shoots per propagule the given trait (2.30). This finding may be attributed to cytokinin deficiency in stimulation cell division and morphogenesis (shoot initiation/bud formation) in tissue culture and break of apical dominance and release the growth of lateral buds (Stern et al, 2004;Duhoky and Rasheed, 2009). On the other side, NAA had not significant effect on the same trait.…”

Section: Initiation Stagementioning

confidence: 99%

Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

Gaber

Barakat

2019

Alexandria Science Exchange Journal

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Gardenia jasminoidesEllis is an evergreen plant with fragrant creamy white flowers and glossy, darkgreen leaves, belongs to family Rubiaceae and member of the genus Gardenia. The current study aimed to afford a new technique for in vitro propagation and callus induction of G. jasminoides by using single nodes excised from soft cuttings using full strength MS salts supplemented with 30g/l sucrose, 4g/l gelrite and different concentrations of plant growth regulators. Generally, the current study showed that NAA and BA at 0.0 and 1.0 mg/l, respectively gave rise to the best results for initiation stage. Meanwhile, fortified the basal MS medium with BA and NAA at 2.0 and 0.250 mg/l, consecutively; resulted in the best results with respect to multiplication stage. Regarding rhizogenesis, the neoformed shoots produced from vegetative multiplication stage were rooted successfully upon cultured on MS medium supplemented with 1.0 mg/l IBA and NAA at either 0.250 or 0.500 mg/l, whereas the highest number of roots was recorded besides callus induction. Regarding embryonic callus induction, adding 2,4-D at 1.0 mg/l; enhanced embryonic callogenesis. Neoformed plantlets were acclimatized ex vitro and in vivo vigorously in mixture of perlite and peatmoss at (1:1, v/v), which resulted in the highest percentage value of survival / percent (100%) and successfully flowered and showed true-to-type plants ex vitro.

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Section: Initiation Stagementioning

confidence: 99%