Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

Abstract

Gardenia jasminoidesEllis is an evergreen plant with fragrant creamy white flowers and glossy, darkgreen leaves, belongs to family Rubiaceae and member of the genus Gardenia. The current study aimed to afford a new technique for in vitro propagation and callus induction of G. jasminoides by using single nodes excised from soft cuttings using full strength MS salts supplemented with 30g/l sucrose, 4g/l gelrite and different concentrations of plant growth regulators. Generally, the current study showed that NAA and BA at 0.0 and 1.0 mg/l, respectively gave rise to the best results for initiation stage. Meanwhile, fortified the basal MS medium with BA and NAA at 2.0 and 0.250 mg/l, consecutively; resulted in the best results with respect to multiplication stage. Regarding rhizogenesis, the neoformed shoots produced from vegetative multiplication stage were rooted successfully upon cultured on MS medium supplemented with 1.0 mg/l IBA and NAA at either 0.250 or 0.500 mg/l, whereas the highest number of roots was recorded besides callus induction. Regarding embryonic callus induction, adding 2,4-D at 1.0 mg/l; enhanced embryonic callogenesis. Neoformed plantlets were acclimatized ex vitro and in vivo vigorously in mixture of perlite and peatmoss at (1:1, v/v), which resulted in the highest percentage value of survival / percent (100%) and successfully flowered and showed true-to-type plants ex vitro.

Transformasi Genetik pada Kalus Embriogenik Tanaman Suku Rubiaceae

“…Tanaman yang dihasilkan melalui kultur in vitro mampu meningkatkan hasil panen hingga 20,4% lebih banyak dibanding tanaman hasil perbanyakan tradisional (Robinson et al, 1993). Penggunaan teknik terminal cutting sebagai salah satu contoh propagasi konvensional tanaman Rubiaceae juga diketahui dapat menyebabkan terjadinya penurunan laju multiplikasi (Gaber & Barakat at al., 2019). Kendala di atas dapat diatasi dengan propagasi in vitro yang mampu menghasilkan tanaman dalam jumlah yang banyak dengan waktu yang singkat.…”

Regeneration, in Vitro Mutation and Evaluation of Genetic Stability of Gardenia Somaclones Via Ssr Markers

“…Among the means of developing plant species and enriching their characteristics is that genetic diversity that can be created through convinced chemical mutations to gain new plants with better characteristics. (Suprasanna et al, 2015;Gaber and Barakat, 2019). Ethyl Methane Sulphonate (EMS) is one of the most robust chemical mutagens used in plants.…”