Effect of Media on Spore Yield and Thermal Resistance of Bacillus stearothermophilus

Penna, Thereza Christina Vessoni; Ishii, Marina; Machoshvili, Irene Alexeevna

doi:10.1007/978-1-4612-0057-4_23

Cited by 4 publications

(6 citation statements)

References 3 publications

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“…However, what kind of components of yeast extract can affect spores viability is still not clear. Penna et al found that increasing concentrations of yeast extract in the sporulation medium enhanced heat resistance of spores [23]. This founding is consistent with our results, because the determination of both viable spore yield and spore viability in our experiment is based on enumerating colony forming bacteria through plate counting after a heat treatment (60 °C, 30 min) to the spore samples.…”

Section: Discussionsupporting

confidence: 91%

Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

et al. 2017

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An optimized strategy for the enhancement of microbially induced calcium precipitation including spore viability ensurance, nutrient selection and O 2 supply was developed. Firstly, an optimal yeast extract concentration of 5 g/L in sporulation medium was determined based on viable spore yield and spore viability. Furthermore, the effects of certain influential factors on microbial calcium precipitation process of H4 in the presence of oxygen releasing tablet (ORT) were evaluated. The results showed that CaO 2 is preferable to other peroxides in improving the calcium precipitation by H4. H4 strain is able to precipitate a highly insoluble calcium at the CaO 2 dosage range of 7.5-12.5 g/L, and the most suitable spore concentration is 6 × 10 8 spores/ml when the spore viability (viable spore ratio) is approximately 50%. Lactate is the best carbon source and nitrate is the best nitrogen source for aerobic incubation. This work has laid a foundation of ternary self-healing system containing bacteria, ORT, and nutrients, which will be promising for the self-healing of cracks deep inside the concrete structure.

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Section: Discussionsupporting

confidence: 91%

Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

et al. 2017

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“…While research continues to further elucidate the mechanisms associated with wet [184][185][186] and dry heat 8,181,187 spore inactivation, thermal treatment applied on a large scale may be impractical. 14 Nonetheless,Buhr et al 188 demonstrated decontamination of a C-130 aircraft using dry heat (75)(76)(77)(78)(79)(80).…”

Section: Physical-based Decontaminants Thermal Treatmentmentioning

confidence: 99%

Review of Decontamination Techniques for the Inactivation of Bacillus anthracis and Other Spore-Forming Bacteria Associated with Building or Outdoor Materials

Wood

Adrion

2019

Environ. Sci. Technol.

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Since the intentional release of Bacillus anthracis spores through the U.S. Postal Service in the fall of 2001, research and development related to decontamination for this biological agent have increased substantially. This review synthesizes the advances made relative to B. anthracis spore decontamination science and technology since approximately 2002, referencing the open scientific literature and publicly available, well-documented scientific reports. In the process of conducting this review, scientific knowledge gaps have also been identified. This review focuses primarily on techniques that are commercially available and that could potentially be used in the large-scale decontamination of buildings and other structures, as well as outdoor environments. Since 2002, the body of scientific data related to decontamination and microbial sterilization has grown substantially, especially in terms of quantifying decontamination effcacy as a function of several factors. Specifically, progress has been made in understanding how decontaminant chemistry, the materials the microorganisms are associated with, environmental factors, and microbiological methods quantitatively impact spore inactivation. While advancement has been made in the past 15 years to further the state of the science in the inactivation of bacterial spores in a decontamination scenario, further research is warranted to close the scientific gaps that remain.

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“…Other factors can affect the resistance of the spores of individual strains, including the levels of nutrients or divalent cations in sporulation media (Dadd et al . 1983; Gerhardt and Marquis 1989; Penna et al . 2003).…”

Section: Introductionmentioning

confidence: 99%

“…One such factor is the optimum cell-growth temperature, as spores of thermophilic species have higher wet heat resistance than spores of mesophilic species, which is in turn higher than that of the spores of psychrophilic species (Warth 1978;Gerhardt and Marquis 1989). Other factors can affect the resistance of the spores of individual strains, including the levels of nutrients or divalent cations in sporulation media (Dadd et al 1983;Gerhardt and Marquis 1989;Penna et al 2003). Even when the same medium is used, the precise sporulation conditions can affect the properties of the spores of the same strain.…”

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confidence: 99%

Comparison of the properties of Bacillus subtilis spores made in liquid or on agar plates

et al. 2007

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Aims: To compare the properties of the spores of Bacillus subtilis prepared in liquid and on plates. Methods and Results: The spores of B. subtilis were prepared at 37°C using a nutrient exhaustion medium either in liquid or on agar plates. The levels of core water, dipicolinic acid (DPA) and small, acid‐soluble spore proteins (SASP) were essentially identical in spores made in liquid or on plates. Spores prepared in liquid were killed ∼threefold more rapidly at 90°C in water than the spores prepared on plates, and the spores prepared in liquid were more sensitive to nitrous acid and a diluted stable superoxidized water. Spores prepared in liquid also germinated more rapidly with several agents than those prepared on plates. Pellets of spores prepared on plates were darker than spores prepared in liquid, and spores prepared in liquid had more readily extracted coat protein. However, there were no major differences in the relative levels of individual coat proteins or the cross‐linking of the coat protein GerQ in the two types of spores, although the inner membrane of spores prepared on plates had a higher ratio of anteiso‐ to iso‐fatty acids. Conclusions: The preparation in liquid yielded spores with some different properties than those made on agar plates. Spores made in liquid had lower resistance to heat and several chemicals, and germinated more readily with several agents. There were also differences in the composition of the inner membrane of spores prepared under these two conditions. However, there were no major differences in the levels of DPA, core water, SASP and individual coat proteins or the cross‐linking of a coat protein in spores made in liquid and on plates. Significance and Impact of the Study: This work demonstrates that the preparation method can affect the resistance and germination properties of bacterial spores, even if an identical medium and temperature are used. Evidence was also obtained consistent with the role of the inner membrane in spore resistance and germination, and that some factor in addition to core water, DPA and SASP content plays a role in spore resistance to wet heat.

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Effect of Media on Spore Yield and Thermal Resistance of Bacillus stearothermophilus

Cited by 4 publications

References 3 publications

Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

Review of Decontamination Techniques for the Inactivation of Bacillus anthracis and Other Spore-Forming Bacteria Associated with Building or Outdoor Materials

Comparison of the properties of Bacillus subtilis spores made in liquid or on agar plates

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