2017
DOI: 10.3390/ma10020116
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Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

Abstract: An optimized strategy for the enhancement of microbially induced calcium precipitation including spore viability ensurance, nutrient selection and O 2 supply was developed. Firstly, an optimal yeast extract concentration of 5 g/L in sporulation medium was determined based on viable spore yield and spore viability. Furthermore, the effects of certain influential factors on microbial calcium precipitation process of H4 in the presence of oxygen releasing tablet (ORT) were evaluated. The results showed that CaO 2… Show more

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Cited by 38 publications
(14 citation statements)
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“…Nevertheless, in a series of recent studies the potential of oxygen releasing compounds, typically peroxides, as part of concrete healing agent formulations were investigated for their potential to increase calcium carbonate precipitation yield by aerobic bacteria under oxygen‐limiting conditions. While calcium peroxide and zinc peroxide appeared inhibitory, urea–hydrogen peroxide and magnesium peroxide were found to stimulate calcium carbonate precipitation in one study, Zhang et al found that calcium peroxidase tablets improved calcium precipitation by bacterial strain H4 at a dosage range of 7.5–12.5 g L −1 …”
Section: Self‐healing Bioconcretementioning
confidence: 99%
“…Nevertheless, in a series of recent studies the potential of oxygen releasing compounds, typically peroxides, as part of concrete healing agent formulations were investigated for their potential to increase calcium carbonate precipitation yield by aerobic bacteria under oxygen‐limiting conditions. While calcium peroxide and zinc peroxide appeared inhibitory, urea–hydrogen peroxide and magnesium peroxide were found to stimulate calcium carbonate precipitation in one study, Zhang et al found that calcium peroxidase tablets improved calcium precipitation by bacterial strain H4 at a dosage range of 7.5–12.5 g L −1 …”
Section: Self‐healing Bioconcretementioning
confidence: 99%
“…The utilization of bacteria to fill up the concrete pores or cracks by self-healing process has been reported by authors in the literature [8,9], The technique is used in order to accelerate the precipitation of calcium carbonate (CaCO 3 ) on the bacterial cell wall to increase the compressive and splitting tensile strength as well as reduction in water penetration [8][9][10][11][12][13]. The bacteria increase the formation of CaCO 3 layer as a function for diffusing CO 2(g) through the wet concrete and production of HCO 3 -(aq) as an intermediate product.…”
Section: Introductionmentioning
confidence: 99%
“…Among these different forms, calcite is the most stable form of calcium carbonate (Rao et al, 2013). The chemical reaction for the formation of calcium carbonate by ''Bacillus subtilis'' microorganisms is shown below in equation 2, in which calcium lactate reacts with oxygen and converted into calcium carbonate, carbon dioxide gas, and water (Zhang et al, 2017) CaC 6 H 10 O 6 + 6O 2 ! CaCO 3 + 5CO 2 + 5H 2 0 ð2Þ Figure 8 shows the microstructure of F-4 having sand as a carrier material.…”
Section: Microstructure Analysismentioning
confidence: 99%