Effect of media rheology and bioreactor hydrodynamics on filamentous fungi fermentation of lignocellulosic and starch-based substrates under pseudoplastic flow conditions

Osadolor, Osagie Alex; Jabbari, Mostafa; Nair, Ramkumar B.; Lennartsson, Patrik R.; Taherzadeh, Mohammad J.

doi:10.1016/j.biortech.2018.04.093

Cited by 14 publications

(4 citation statements)

References 30 publications

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“…The flasks were constantly agitated in an orbital agitator (BOECO ES-20/80, Germany), and for cultivation time, biomass samples were taken every 24 h. In the other conditions, the samples were taken at the set time by the culture time test as it was where the highest amount of β-glucans was produced. The biomass was washed with deionized water repeatedly until it was free of the culture broth, subsequently dried at 65 °C for 24 h, allowed to cool at room temperature and weighed until later use in the extraction process of polysaccharides from the cell walls 46 .…”

Section: Determination Of Physical Fermentation Parametersmentioning

confidence: 99%

Polyphasic identification of Rhizopus oryzae and evaluation of physical fermentation parameters in potato starch processing liquid waste for β-glucan production

Anchundia,

León-Revelo,

Santacruz

et al. 2024

Sci Rep

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AbstractΒ-glucans are polysaccharide macromolecules that can be found in the cell walls of molds, such as Rhizopus oryzae. They provide functional properties in food systems and have immunomodulatory activity, anticancer, and prebiotic effects; reduce triglycerides and cholesterol; and prevent obesity, among others benefits. Furthermore, potato starch production requires a large amount of water, which is usually discharged into the environment, creating problems in soils and bodies of water. The physical parameters to produce β-glucans were determined, liquid waste from potato starch processing was used and native Rhizopus oryzae was isolated and identified from cereal grains. The isolates grew quickly on the three types of agars used at 25 °C and 37 °C, and they did not grow at 45 °C. Rhizopus oryzae M10A1 produced the greatest amount of β-glucans after six days of culture at 30 °C, pH 6, a stirring rate of 150 rpm and a fermentation volume of 250 mL. By establishing the physical fermentation parameters and utilizing the liquid waste from potato starch, Rhizopus oryzae M10A1 yielded 397.50 mg/100 g of β-glucan was obtained.

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Section: Determination Of Physical Fermentation Parametersmentioning

confidence: 99%

Polyphasic identification of Rhizopus oryzae and evaluation of physical fermentation parameters in potato starch processing liquid waste for β-glucan production

Anchundia,

León-Revelo,

Santacruz

et al. 2024

Sci Rep

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“…Both the extension of the duration of the continuous bioprocess and the mass of the accumulated biomass are key for a bioprocess that could achieve sufficient productivity and scalability. The control of the mycelial morphology through bioreactor design and contemporary fermentation strategy is therefore both of paramount importance (Osadolor et al 2018 ). Minimising biomass adhesion to bioreactor components (e.g.…”

Section: Introductionmentioning

confidence: 99%

Airlift bioreactor–based strategies for prolonged semi-continuous cultivation of edible Agaricomycetes

Cerrone,

Lochlainn,

Callaghan

et al. 2024

Appl Microbiol Biotechnol

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Submerged cultivation of edible filamentous fungi (Agaricomycetes) in bioreactors enables maximum mass transfer of nutrients and has the potential to increase the volumetric productivity of fungal biomass compared to solid state cultivation. These aspects are paramount if one wants to increase the range of bioactives (e.g. glucans) in convenient time frames. In this study, Trametes versicolor (M9911) outperformed four other Agaricomycetes tested strains (during batch cultivations in an airlift bioreactor). This strain was therefore further tested in semi-continuous cultivation. Continuous and semi-continuous cultivations (driven by the dilution rate, D) are the preferred bioprocess strategies for biomass production. We examined the semi-continuous cultivation of T. versicolor at dilution rates between 0.02 and 0.1 h−1. A maximum volumetric productivity of 0.87 g/L/h was obtained with a D of 0.1 h−1 but with a lower total biomass production (cell dry weight, CDW 8.7 g/L) than the one obtained at lower dilution rates (12.3 g/L at D of 0.04 and vs 13.4 g/L, at a D of 0.02 h−1). However, growth at a D of 0.1 h−1 resulted in a very short fermentation (18 h) which terminated due to washout (the specific D exceeded the maximum growth rate of the fungal biomass). At a D of 0.04 h−1, a CDW of 12.3 g/L was achieved without compromising the total residence time (184 h) of the fermentation. While the D of 0.04 h−1 and 0.07 h−1 achieved comparable volumetric productivities (0.5 g/L/h), the total duration of the fermentation at D of 0.07 h−1 was only 85 h. The highest glucan content of cells (27.8 as percentage of CDW) was obtained at a D of 0.07 h−1, while the lowest glucan content was observed in T. versicolor cells grown at a D of 0.02 h−1. Key points • The highest reported volumetric productivity for fungal biomass was 0.87 g/L/h. • Semi-continuous fermentation at D of 0.02 h−1 resulted in 13.4 g/L of fungal biomass. • Semi-continuous fermentation at D of 0.07 h−1 resulted in fungal biomass with 28% of total glucans.

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“…Airlift bioreactors and fluidized bed reactors can bring about mixing and mass transfer while avoiding mechanical stress, and are commonly used for aerobic cultivation conditions. However, the control of mixing using upward bubble movement leads to sedimentation or wash-out when the aeration rate is too low or too high, respectively [19,20]. Regardless, different agitation and aeration systems (i.e., mechanical agitation vs. airlift) have strong effects on fungal morphology (i.e., mycelia vs. pellet vs. clump formation).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Raspberry Ketone Production via Submerged Fermentation in Different Bioreactors

Zhang

Peterson

et al. 2023

Fermentation

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Raspberry ketone (RK) has high commercial value in the food and healthcare industries. A biological route to this flavour compound is an attractive prospect, considering the need to meet consumer demands and sustainable goals; however, it is yet to become an industrial reality. In this work, fungal production of raspberry ketone (RK) and raspberry compounds (RC) via submerged fermentation of Nidula niveo-tomentosa was characterized in flask, stirred-tank reactor (STR), panel bioreactor (PBR), and fluidized bed reactor (FBR) configurations. The results indicate that the panel bioreactor resulted in larger, floccose pellets accompanied by maximum titres of 20.6 mg/L RK and 50.9 mg/L RC. The stirred-tank bioreactor with impeller mixing yielded compact elliptical pellets, induced the highest volumetric productivity of 2.0 mg L−1 day−1, and showed RK selectivity of 0.45. While differing mixing strategies had clear effects on pellet morphology, RK production presented a more direct positive relationship with cultivation conditions, and showed appropriate mixing and aeration favour RK to raspberry alcohol (RA). Overall, this paper highlights the importance of bioreactor design to fungal fermentation, and gives insight into green and industrial bioproduction of value-added natural compounds.

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Effect of media rheology and bioreactor hydrodynamics on filamentous fungi fermentation of lignocellulosic and starch-based substrates under pseudoplastic flow conditions

Cited by 14 publications

References 30 publications

Polyphasic identification of Rhizopus oryzae and evaluation of physical fermentation parameters in potato starch processing liquid waste for β-glucan production

Polyphasic identification of Rhizopus oryzae and evaluation of physical fermentation parameters in potato starch processing liquid waste for β-glucan production

Airlift bioreactor–based strategies for prolonged semi-continuous cultivation of edible Agaricomycetes

Comparison of Raspberry Ketone Production via Submerged Fermentation in Different Bioreactors

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