Effect of Melatonin Intake on Oxidative Stress Biomarkers in Male Reproductive Organs of Rats under Experimental Diabetes

Abstract

This study investigated the antioxidant system response of male reproductive organs during early and late phases of diabetes and the influence of melatonin treatment. Melatonin was administered to five-week-old Wistar rats throughout the experiment, in drinking water (10 μg/kg b.w). Diabetes was induced at 13 weeks of age by streptozotocin (4.5 mg/100 g b.w., i.p.) and animals were euthanized with 14 or 21 weeks old. Activities of catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and lipid peroxidation were evaluated in prostate, testis, and epididymis. The enzymes activities and lipid peroxidation were not affected in testis and epididymis after one or eight weeks of diabetes. Prostate exhibited a 3-fold increase in GPx activity at short-term diabetes and at long-term diabetes there were 2- and 3-fold increase in CAT and GST, respectively (p ≤ 0.01). Melatonin treatment to healthy rats caused a 47% increase in epididymal GPx activity in 14-week-old rats. In prostate, melatonin administration normalized GST activity at both ages and mitigated GPx at short-term and CAT at long-term diabetes. The testis and epididymis were less affected by diabetes than prostate. Furthermore, melatonin normalized the enzymatic disorders in prostate demonstrating its effective antioxidant role, even at low dosages.

Tualang Honey Protects the Rat Midbrain and Lung against Repeated Paraquat Exposure

“…Taken together, these phenomena may explain the significant reduction in GPx but not CAT in the present study, given that GPx may respond faster than CAT, particularly during the early stages of the disease. A recent study conducted by Gobbo et al [62] showed similar findings, with activation of GPx occurring early during the response to diabetes, whereas activation of CAT tended to occur at later stages of untreated diabetes. Further research is needed to understand the effects of PQ on GPx and CAT activities in the midbrain.…”

Diyabetik Sıçanlarda Melatonin Uygulamasının Karaciğer, Böbrek, Mide, Pankreas ve Göz Dokularında Oksidatif Stres Üzerine Etkisi

“…Melatonin hidroksil radikali, superoksit radikali ve peroksinitrit gibi SOR türevlerini olarak ortadan kaldırarak direkt antioksidan etki gösterirken, endojen antioksidan enzimlerin yapımını artırarak indirekt olarak antioksidan etki de göstermektedir (21).…”

Diabetes mellitus-induced male reproductive impairment: The role of natural products: A review

“…A similar trend of results is reported by Hamden et al (2008) using alloxan-induced T1DM rats. On the other hand, Gobbo et al (2015) have reported that DM did not significantly alter the testicular antioxidant defence system as shown by the insignificant alterations in the activities of antioxidant enzymes (SOD, CAT, GPx) and MDA level after 1 week and 8 weeks in STZ-induced T1DM rats.…”

“…Several studies using STZ-induced T1DM animal model have shown decreased daily sperm production, sperm count and motility (Suresh and Prakash, 2012;Gonzales et al, 2013), and increased percentage of spermatozoa with abnormal morphology (Kanter et al, 2013). Studies using alloxan-induced T1DM animal models (Hafez 2010;Ghlissi et al, 2012) have also yielded similar results.…”

“…Spermatogenesis is negatively affected when steroidogenesis is suppressed, as observed in DM. Studies using whole plant extracts (Mallick et al, 2010;Hafez 2010;Gonzales et al, 2013) and compounds of plant origin (Khaki et al, 2009;Roy et al, 2013) have shown a significant improvement in spermatogenesis, in which sperm count, normal morphology, viability and motility are significantly increased (Tables 1 and 2). Furthermore, curcumin (Kanter et al, 2013) and quercetin (Kanter et al, 2012) have improved spermatogenesis, as shown by an increase in mean testicular biopsy score after 8 weeks of treatment.…”