Effect of menstrual cycle on HIV-1 levels in the peripheral blood and genital tract

Reichelderfer, Patricia; Coombs, Robert W.; Wright, David J.; Cohn, Jonathan; Burns, David; Cu‐Uvin, Susan; Baron, Penny; Cohen, Mardge H.; Landay, Alan; Beckner, Suzanne K.; Lewis, Shirley; Kovács, Andrea

doi:10.1097/00002030-200009290-00005

Cited by 108 publications

(73 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Seminal plasma was pretreated with silica gel before being subjected to RT-PCR. Endocervical fluid was obtained using Sno-Strip wicks (36). Blood CD4 ϩ T-cell counts were enumerated by standard consensus flow cytometry.…”

Section: Study Populationmentioning

confidence: 99%

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes

Musey¹,

Ding²,

Cao³

et al. 2003

J Virol

View full text Add to dashboard Cite

Induction of adaptive immunity to human immunodeficiency virus type 1 (HIV-1) at the mucosal site of transmission is poorly understood but crucial in devising strategies to control and prevent infection. To gain further understanding of HIV-1-specific T-cell mucosal immunity, we established HIV-1-specific CD8؉ cytotoxic T-lymphocyte (CTL) cell lines and clones from the blood, cervix, rectum, and semen of 12 HIV-1-infected individuals and compared their specificities, cytolytic function, and T-cell receptor (TCR) clonotypes. Blood and mucosal CD8؉ CTL had common HIV-1 epitope specificities and major histocompatibility complex restriction patterns. Moreover, both systemic and mucosal CTL lysed targets with similar efficiency, primarily through the perforin-dependent pathway in in vitro studies. Sequence analysis of the TCR␤ VDJ region revealed in some cases identical HIV-1-specific CTL clones in different compartments in the same HIV-1-infected individual. These results clearly establish that a subset of blood and mucosal HIV-1-specific CTL can have a common origin and can traffic between anatomically distinct compartments. Thus, these effectors can provide immune surveillance at the mucosa, where rapid responses are needed to contain HIV-1 infection.Human immunodeficiency virus type 1 (HIV-1) transmission occurs predominantly within the genital and rectal mucosa through sexual contact. Virus-specific T cells, known to control HIV-1 infection systemically (21, 29, 37), may also facilitate viral clearance in the mucosa. We previously identified class I major histocompatibility complex (MHC)-restricted CD8 ϩ cytotoxic T lymphocytes (CTL) in the cervices of infected women that recognize and lyse HIV-1-infected cells. HIV-1-specific cervical CTL were more frequently detected in patients with preserved rather than decreased CD4 ϩ T-cell counts, suggesting that the CD8 ϩ effectors provide immune surveillance against local viral replication (28). More recently, we and others have extended these findings to semen and the rectal lymphoid tissue of infected men (19,34,38,39).The importance of understanding mechanisms to control infection locally is underscored by recent attention to the distinct properties of HIV-1 within the mucosa. Investigators comparing HIV-1 in blood and mucosal secretions (cervix and semen) have highlighted significant differences in viral phenotypes and genotypes as well as clearance following combination antiretroviral therapy (7,12,20,32,41,43,44). However, although mucosal antibody responses can differ from systemic responses (26), such discrepancies pertaining to HIV-1-specific T cells have not been elucidated. We previously demonstrated that most T cells in the female lower reproductive tract express T-cell receptor ␣␤ (TCR␣␤) (15, 28), similar to cells in the circulation and lymph nodes. Nevertheless, it is unclear whether HIV-1-specific CTL in the genital and gastrointestinal tracts emerge distinct from their systemic counterparts. Additionally, it is not known if mucosal CTL, similar to syste...

show abstract

Section: Study Populationmentioning

confidence: 99%

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes

Musey¹,

Ding²,

Cao³

et al. 2003

J Virol

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4] In regions of the world where HIV-1 incidence is highest, more women are newly infected per year than men. 5 While social, behavioral, and economic conditions are certainly factors in the increasing incidence of HIV-1 in women, there has been an emphasis on the role of endogenous 6 and exogenous 7 hormones as cofactors in HIV-1 acquisition or infection and disease progression. Observational and prospective longitudinal studies support that pregnancy and lactation, characterized by high serum progesterone (4-pregnene-3,20-dione or P4) and low serum estradiol [1,3,5(10)-estratriene-3,17beta-diol or E2] levels, are also associated with an increased risk of HIV-1 acquisition compared with nonlactating nonpregnant controls.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Follicular and Luteal Phase Mucosal Markers of HIV Susceptibility in Healthy Women

Thurman

Chandra

Yousefieh

et al. 2016

AIDS Research and Human Retroviruses

View full text Add to dashboard Cite

The purpose of this study was to evaluate differences in vaginal immune cell populations, vaginal tissue gene expression, antimicrobial activity of the cervicovaginal (CV) lavage (CVL), vaginal flora, and p24 antigen production from CV tissues after ex vivo human immunodeficiency virus (HIV) infection between follicular (FOL) and luteal (LUT) phases of the menstrual cycle. CV tissue biopsies, CV secretions, and blood samples were obtained as part of two longitudinal clinical trials of healthy women (CONRAD D11-119 and A12-124 studies). Participants (n = 39) were HIV-seronegative women not using exogenous hormone supplementation, with normal menstrual cycles, who were screened to exclude sexually transmitted and reproductive tract infections. Serum levels of estradiol and progesterone were significantly higher in the LUT versus the FOL phase of the menstrual cycle. Controlling for race, reported contraceptive use/sexual practices, and clinical trial, we found no differences in vaginal tissue immune cell populations and activation status, transcriptomes, inhibition of HIV, herpes simplex virus type 2 and Escherichia coli by the CVL, vaginal pH or Nugent score, or production of p24 antigen after ex vivo infection by HIV-1 BaL between CV samples obtained in the FOL phase versus the LUT phase of the menstrual cycle. There were no significant correlations between serum estradiol and progesterone levels and CV endpoints. The hypothesis that the LUT phase of the menstrual cycle represents a more vulnerable stage for mucosal infection with HIV was not supported by data from samples obtained from the lower genital tract (ectocervix and vagina) from these two clinical trials.

show abstract

“…But, the transmission is also possible from mother to child during pregnancy, at childbirth or during breastfeeding. A high viral load (>1500 virus/mL) [40], careless sexual behavior [41e44] and some periods of the menstrual cycle [45,46] are all factors that may increase the chances of transmission.…”

Section: Transmissionmentioning

confidence: 99%

Viral surface glycoproteins, gp120 and gp41, as potential drug targets against HIV-1: Brief overview one quarter of a century past the approval of zidovudine, the first anti-retroviral drug

Teixeira

Gomes

et al. 2011

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

Effect of menstrual cycle on HIV-1 levels in the peripheral blood and genital tract

Cited by 108 publications

References 29 publications

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes

Comparison of Follicular and Luteal Phase Mucosal Markers of HIV Susceptibility in Healthy Women

Viral surface glycoproteins, gp120 and gp41, as potential drug targets against HIV-1: Brief overview one quarter of a century past the approval of zidovudine, the first anti-retroviral drug

Contact Info

Product

Resources

About

Effect of menstrual cycle on HIV-1 levels in the peripheral blood and genital tract

Cited by 108 publications

References 29 publications

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8+Cytotoxic T Lymphocytes

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8+Cytotoxic T Lymphocytes

Comparison of Follicular and Luteal Phase Mucosal Markers of HIV Susceptibility in Healthy Women

Viral surface glycoproteins, gp120 and gp41, as potential drug targets against HIV-1: Brief overview one quarter of a century past the approval of zidovudine, the first anti-retroviral drug

Contact Info

Product

Resources

About

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes

Ontogeny and Specificities of Mucosal and Blood Human Immunodeficiency Virus Type 1-Specific CD8⁺Cytotoxic T Lymphocytes