Effect of mercury on porcine ovarian granulosa cells<i>in vitro</i>

Roychoudhury, Shubhadeep; Massányi, Peter; Slivkova, Jana; Formicki, Grzegorz; Lukáč, Norbert; Slamečka, J.; Sláma, Petr; Kolesárová, Adriana

doi:10.1080/10934529.2015.1019805

Cited by 10 publications

(5 citation statements)

References 52 publications

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“…An increasing trend of apoptosis of granulosa cells was confirmed. Results of this study also confirm a direct effect of mercury on the release of steroid hormone progesterone as well as interference of mercury in the pathways of steroidogenesis and apoptosis [ 161 ]. Other data confirm that mercury administration has a dose-dependent association with the hormonal release by porcine ovarian granulosa cells [ 162 ].…”

Section: Mercury (Hg)supporting

confidence: 73%

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Massányi

Madeddu

et al. 2020

Toxics

150

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Reproductive organs are essential not only for the life of an individual but also for the survival and development of the species. The response of reproductive organs to toxic substances differs from that of other target organs, and they may serve as an ideal “barometer” for the deleterious effects of environmental pollution on animal and human health. The incidence of infertility, cancers, and associated maladies has increased in the last fifty years or more, while various anthropogenic activities have released into the environment numerous toxic substances, including cadmium, lead, and mercury. Data from epidemiological studies suggested that environmental exposure to cadmium, lead, and mercury may have produced reproductive and developmental toxicity. The present review focused on experimental studies using rats, mice, avian, and rabbits to demonstrate unambiguously effects of cadmium, lead, or mercury on the structure and function of reproductive organs. In addition, relevant human studies are discussed. The experimental studies reviewed have indicated that the testis and ovary are particularly sensitive to cadmium, lead, and mercury because these organs are distinguished by an intense cellular activity, where vital processes of spermatogenesis, oogenesis, and folliculogenesis occur. In ovaries, manifestation of toxicity induced by cadmium, lead, or mercury included decreased follicular growth, occurrence of follicular atresia, degeneration of the corpus luteum, and alterations in cycle. In testes, toxic effects following exposure to cadmium, lead, or mercury included alterations of seminiferous tubules, testicular stroma, and decrease of spermatozoa count, motility and viability, and aberrant spermatozoa morphology.

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Section: Mercury (Hg)supporting

confidence: 73%

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Massányi

Madeddu

et al. 2020

Toxics

150

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“…Moreover, the level of progesterone liberated was inhibited from the ovarian granulosa cells of pigs exposed to mercury. This confirms a direct effect of mercury on the secretory release of progesterone as well as interference of Hg in the pathways of steroidogenesis and apoptosis (Knazicka et al 2013;Roychoudhury et al 2015). In the current work, a highly significant decrease in the levels of serum FSH, LH, estrogen, and progesterone was recorded among HgCl2-treated mother rats and their offspring.…”

Section: Discussionsupporting

confidence: 84%

“…The obtained result goes parallel with the finding of rats to two high doses of HgCl2 (5 and 10 mg/kg b.wt) for 48 hours is implicated in the induction of cellular apoptosis in both testes and kidneys (Massanyi et al 2007). The apoptotic effect of mercury is fundamentally attributed to its direct effect on the release of steroid hormones and interference with the pathways of steroidogenesis resulting in apoptosis (Roychoudhury et al 2015).…”

Section: Heath Et Al Studied the Effects Of Two Doses Ofmentioning

confidence: 99%

Modulatory role of celery (Apium graveolens) leaves extract against mercuric chloride-induced gonadal dysfunction in female rats and their pups

El-Beltagy

Elattar

Abdelaziz

et al. 2020

Journal of Medical and Life Science

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Background: Mercury (Hg) is a toxic heavy metal that has been recognized as one of the strongest environmental pollutants. Exposure to Hg even in low doses can induce adverse effects on the structure and functions of the different body organs including gonads. Celery (Apium graveolens) leaves are rich with antioxidant compounds like apiin, apigenin, and quercetin, and vitamins like E and C. Aim: This work aimed to evaluate the possible modulatory role of celery leaves extract against gonadal toxicity induced by HgCl2 in female rats and their pups during gestation and lactation periods. Material &methods: Twenty-four female Wistar rats (180-200g) were used in this experiment.They were mated with males. After confirmation of the gestation test, the pregnant rats were divided into four groups (six for each) as follows: control, celery extract (CE) supplement (200 mg/kg b.wt), HgCl2 (2mg/kg b.wt) and HgCl2 co-supplemented with CE groups. The experiment extended from the 4 th day of gestation till the end of the weaning period. At the end of weaning, the female rats and their pups were weighed and sacrificed to collect the blood and remove the gonads for estimation of biochemical and histopathological changes. Results: The obtained results revealed that exposure of female rats to HgCl2 resulted in a significant decrease in the activities of antioxidant enzymes (SOD, CAT, and GPx) and sexual hormones (FSH, LH, estrogen, progesterone, and testosterone) in both mother rats and their pups if compared with control. Further, a light microscopic investigation revealed that exposure to HgCl2 during gestation and lactation periods induced deleterious histopathological changes in the ovaries of mothers as well as in the ovaries and testes of their offspring. Additionally, the ovarian sections from mothers and their offspring displayed weak to negative expression for calretinin antibody, however, the testicular sections from offspring appeared strongly stained with COX-2 antibody (inflammatory marker) and weakly stained with Ki67 antibody (proliferative marker). Further study by flow cytometric technique revealed a highly significant increase in the mean % value of apoptotic cells in both ovaries and testes. On the other hand, co-supplementation of CE to HgCl2 treated group successfully alleviated the altered antioxidants and hormones as well as the histological changes near to the normal as control. Conclusion: Celery extract has a powerful ameliorative role against HgCl2-induced deleterious biochemical and histopathological changes in the gonads of female rats and their pups.

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“…The involvement of Hg on cellular processes of progesterone secretion has also been studied in mammalian models. On porcine model, previous study showed that HgCl 2 reduced cell viability and inhibited the progesterone synthesis in porcine ovarian granulosa cells (Roychoudhury et al., ).…”

Section: Discussionmentioning

confidence: 96%

Molecular mechanism of mercuric chloride inhibiting progesterone secretion in ovarian granulosa cells of laying hens

Gong

et al. 2018

Animal Physiology Nutrition

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This study investigated the effect of mercury (Hg) on progesterone secretion in ovarian granulosa cells of laying hens. The gene expressions of steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage (P450scc) and 3β-hydroxysteroid dehydrogenase (3β-HSD), cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway and intracellular calcium ion (Ca ) were further investigated to uncover the molecular mechanism. Results revealed that the cell viability was gradually decreased after Hg exposure from 0 to 24 hr. Besides, progesterone secretion was significantly decreased (p < 0.05) as the concentration of Hg increased from 0 to 4 μM followed by a plateau in 6 μM Hg group at 12-hr time point. Compared with 0 μM Hg group, 4 and 6 μM Hg for 48 hr had significantly decreased progesterone secretion (p < 0.05), while Hg exposure for 6 and 24 hr had no apparent effect on progesterone secretion. In addition, positive correlations occurred among intracellular progesterone, cAMP, PKA, mRNA expressions of StAR, P450scc and 3β-HSD at 12-h and 24-h time points. On the contrary, intracellular Ca level was negatively related to cAMP level at 6 time point and was negatively correlated with progesterone and PKA level at 48 time point. It could be concluded that Hg dose- and time-dependently inhibited progesterone secretion by means of attenuating cAMP-PKA signal pathway, gene expressions of StAR, P450scc and 3β-HSD and enhancing intracellular Ca in ovarian granulosa cells of laying hens.

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Effect of mercury on porcine ovarian granulosa cellsin vitro

Cited by 10 publications

References 52 publications

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Modulatory role of celery (Apium graveolens) leaves extract against mercuric chloride-induced gonadal dysfunction in female rats and their pups

Molecular mechanism of mercuric chloride inhibiting progesterone secretion in ovarian granulosa cells of laying hens

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