Effect of mesenchymal stem cells on induced skeletal muscle chemodenervation atrophy in adult male albino rats

Shehata, Azza S.; Al-Ghonemy, Nabila M.; Ahmed, Sabah M.; Mohamed, Samar R.

doi:10.1016/j.biocel.2017.01.016

Cited by 3 publications

(2 citation statements)

References 86 publications

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“…Murad et al, [81] mentioned that L-Carnitine occupy a vital role in the mitochondrial transport and oxidation of fatty acids. Shehata et al, [82] stated that L-carnitine defend against mitochondrial malfunctions accompanying oxidative strain caused by many factors such as aging, ischemia reperfusion, inflammation, degenerative diseases, carcinogenesis and drug toxicity, in vivo or in vitro. Its mechanism of action was explained by Oyanagi et al, [27] who mentioned that it avoids apoptosis pathway by stimulating mitochondrial respiration and encouraging of cell energy manufacture via β-oxidation in the mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Effect of Simvastatin on the Skeletal Muscles of Senile Male Albino Rats and Possible Protective Role of L- Carnitine. A Histological Study

Mehanna

Allah

Al-Shahed

et al. 2019

Egyptian Journal of Histology

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Background: Hyperlipidemia is a disorder in metabolism which means an abnormal increase in levels of lipids (as cholesterol) and lipid-protein in the blood. It is one of the risk factors that mainly cause atherosclerosis, myocardial infarction, coronary heart disease, cerebral stroke, and renal failure. Simvastatin (S) is one of the most prescribed drugs frequently all over the world due to its excellent performance as hypolipidemic and its relatively low price. L-carnitine (LC) is a nutritional supplement supporting with clinical challenges such as dyslipidemia, anorexia & physical performance. This study aimed to evaluate the protective role of LC on the skeletal muscle of hyperlipidemic rats treated with (S). Materials and Methods: Thirty senile male albino rats were used, divided into five equal groups as follows: control group (GI) and the other four groups were fed the high-fat diet for three weeks till the occurrence of hyperlipidemia. GII: was fed the high-fat diet for three weeks (hyperlipidemic) then sacrificed. GIII: hyperlipidemic rats were then treated orally with (S; 1.5 mg/rat/ /day) for 4 weeks. GIV: hyperlipidemic rats were then treated orally with (LC; 20 mg/rat/day) for 4 weeks. GV: hyperlipidemic rats were then treated with (S) and (LC) at the same time. Samples were taken and processed for light and electron microscopic studies. Results: Application of (S) induced multiple changes of muscles as loss of transverse striations, splitting of myofibrils, and presence of central nuclei. Treatment with (LC) causes improvement of these changes. Conclusion: Application of (LC) improved the degenerative changes of muscle obtained by administration of (S).

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Section: Discussionmentioning

confidence: 99%

Effect of Simvastatin on the Skeletal Muscles of Senile Male Albino Rats and Possible Protective Role of L- Carnitine. A Histological Study

Mehanna

Allah

Al-Shahed

et al. 2019

Egyptian Journal of Histology

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“…Ophthalmologists, dermatologists, and neurologists are widely used the Clostridium botulinum toxin ( Ferreira et al, 2016 , Freedman et al, 2016 ). One of the important applications of botulinum toxin (BTX or Botox) is in Botox and muscle paralysis by inhibiting the release of neurotransmitters such as acetylcholine which make the nerves of the infusion site unable to stimulate the muscle and the relaxation ( Shehata et al, 2017 ). Also, toxic crystals of Bacillus thuringiensis are used as bioinsecticide against agricultural pests ( Palma et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The functions of azurin of Pseudomonas aeruginosa and human mammaglobin-A on proapoptotic and cell cycle regulatory genes expression in the MCF-7 breast cancer cell line

Ghasemi-Dehkordi

Doosti

Jami

2020

Saudi Journal of Biological Sciences

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Azurin protein of Pseudomonas aeruginosa is an anti-tumor agent against breast cancer and mammaglobin-A (MAM-A) protein is a specific antigen on the surface of MCF-7 for induction of cellular immune. The purpose of the present study was to investigate the effects of simultaneous expression of azurin and human MAM-A genes on the mRNA expression level of apoptosis-related and cell cycle genes in MCF-7 breast cancer cell line. The recombinant or empty plasmids were separately transferred into MCF-7 cells using Lipofectamine reagent. Flow cytometry was done to detect cell death and apoptosis. The expression of azurin and MAM-A genes were evaluated by IF assay, RT-PCR and western blot methods. Finally, apoptosis-related and cell cycle genes expression was examined in transformed and non-transformed MCF-7 cells by qPCR method. The successful expression of azurin and MAM-A genes in the MCF-7 cell were confirmed by RT-PCR, IF and western blotting. The apoptosis assay was showed a statistically significant ( p < 0.05) difference after transfection. The expression of BAK , FAS , and BAX genes in transformed cells compare with non-transformed and transformed MCF-7 by pBudCE4.1 were increased statistically significant ( p < 0.05) increases. Although, the increase of SURVIVIN and P53 expressions in transformed cells were not statistically significant ( p > 0.05). Co-expression of azurin and MAM-A genes could induce apoptosis and necrosis in human MCF-7 breast cancer cells by up-regulation of BAK , FAS, and BAX genes. In future researches, it must be better the immune stimulation of pBudCE4.1-azurin-MAM-A recombinant vector in animal models and therapeutic approaches will be evaluated.

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Stem cell biotherapy: A new remedy for Trichinella spiralis-induced inflammatory myopathy

Rayia

Izzularab

Harras

et al. 2023

Parasitology International

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Effect of mesenchymal stem cells on induced skeletal muscle chemodenervation atrophy in adult male albino rats

Cited by 3 publications

References 86 publications

Effect of Simvastatin on the Skeletal Muscles of Senile Male Albino Rats and Possible Protective Role of L- Carnitine. A Histological Study

Effect of Simvastatin on the Skeletal Muscles of Senile Male Albino Rats and Possible Protective Role of L- Carnitine. A Histological Study

The functions of azurin of Pseudomonas aeruginosa and human mammaglobin-A on proapoptotic and cell cycle regulatory genes expression in the MCF-7 breast cancer cell line

Stem cell biotherapy: A new remedy for Trichinella spiralis-induced inflammatory myopathy

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