Effect of Methanolic Extract of &lt;em&gt;Cassia occidentalis&lt;/em&gt; L. Root Bark on Body Weight and Selected Biochemical Parameters in Alloxan Induced Diabetic Rats

Garba, Rahinat; Saidu, Abubakar Ndaman; Adeyemi, H.R.Y.; Muhammad, Hussin

doi:10.19026/bjpt.6.5189

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…and 50% to their feed daily for sixteen days produced significant fasting blood glucose lowering activity in Day 12 and Day 16 dose dependently when compared with the untreated diabetic control, The potency of this effect was seen to be more when compared to that of Glibenclamide as a standard drug. This result is found to be in agreement with that of Garba et al(2015), who reported that various dose levels of the methanolic extracts of Senna occidentalis was able to reduce the blood glucose significantly (p<0.05) but however, not dose-dependently. Furthermore, this is in accordance with work of Emmanuel et al (2011), Verma et al(2011) and Singh et al(2011) who reported that the aqueous extracts of the leaves of S. occidentalis in male albino Wistar rats has shown a significant hypoglycemic activity.…”

Section: Discussionsupporting

confidence: 92%

Effects of <i>Senna occidentalis</i> leaf supplement on blood glucose level, liver enzymes and total protein in alloxan-induced diabetic wistar rats

Gidado¹,

Tanko²,

Sada³

et al. 2016

Bayero J. Pure App. Sci.

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Diabetes mellitus is one of the most common non communicable diseases around the world and is responsible for many deaths resulting from some of its serious complications. Senna occidentalis Linn. is extensively being used in folklore medicine to cure and/or manage many disease conditions, although its adequate validation as therapeutic (specifically anti-diabetic and hepatoprotective) effects has not been established. The aim of this study is to determine the effects of Senna occidentalis leaf supplement on blood glucose level, liver enzymes and total protein in alloxan-induced diabetic wistar rats. Diabetes was induced by single intraperitoneal injection of alloxan (150 mg/kg). Thereafter the rats were fed with Senna occidentalis leaf supplement at doses of 12.5%, 25% and 50% .Also Glibenclamide was used as a standard drug (2 mg/kg) as positive control and diabetic control received distilled water as negative control. Blood glucose levels were measured at day 0, 4, 8, 12 and 16. The liver enzymes and total protein were also determined using the serum and histopathological studies of the pancreas and liver were conducted. Quantitative phytochemical screening revealed that the supplement has high amount of phytoconstitutuents like total phenols, flavonoids, tannins, saponins and alkaloids, which have a percentage composition of 20%, 10.80%, 15%, 0.55% and 7.20%, respectively. Blood glucose levels of all animals treated with the supplement were reduced significantly (p<0.05) by about 82.13%, 88.36 and 81.78% respectively when compared with that of the untreated diabetic control group. There was also an increase in the total protein (p< 0.05) to 90.98 ± 6.02, 73.72 ± 3.02 and 92.23 ± 4.01, respectively in comparison to that of the untreated diabetic group having 69.04 ± 3.59; and hepatotoxicity due to the observed increase in the activities of all the liver enzymes as well as hepatocellular necrosis, sinusoidal congestion and hemorrhage as revealed in the histopathological studies of the liver. This study shows that S. occidentalis leaf supplement has potent hypoglycemic effect due to its high content of active principles that possess strong and potent insulinomimetic and β-cell regenerating potential as depicted by the histopathological studies of the pancreatic tissue, even though, some cytotoxic agents like the cytotoxic saponins may be present in the supplement which is capable of causing damage to both pancreas and liver. These findings prove its use in traditional medicine for the treatment of diabetes but with caution due to its probable potential to induce hepatotoxicity.

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Section: Discussionsupporting

confidence: 92%

Effects of <i>Senna occidentalis</i> leaf supplement on blood glucose level, liver enzymes and total protein in alloxan-induced diabetic wistar rats

Gidado¹,

Tanko²,

Sada³

et al. 2016

Bayero J. Pure App. Sci.

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“…Cassia occidentalis was recognized as one of the widely accepted medicinal plants in traditional medicine, as it yields the desired effect (Yakubu et al, 2007). Besides, every part of the C. occidentalis plant was used for different purposes (Sini et al, 2010) (Sadiq et al, 2012), antioxidant (Daniyan et al, 2011), immunosuppressive andlarvicidal (Abirami et al, 2011) or in wound healing (Garba et al, 2015), sores, itch, cutaneous diseases, bone fracture, fever, ringworm, skin diseases, throat infection (Arya et al, 2010) to mention but a few. Local communities are using the fresh leaves of the plant without prior knowledge of its effect on the body, thus necessitate an effort to assess its safety (Berinyuy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Immunocytotoxic Effect of Aqueous Leaf Extract of Cassia occidentalis on Human Peripheral Blood Mononuclear Cells and Neutrophils

Hamid¹,

Aliyu²,

Isah³

et al. 2021

UJMR

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The study evaluates the potential immunocytotoxic effect of aqueous leaf extract of Cassia occidentalis on human peripheral blood mononuclear cells (PBMCs) and Neutrophils. Different concentrations (25 µg/ml, 50 µg/ml, 100 µg/ml) of the extract were prepared. Six millilitres (6ml) of peripheral blood from consented healthy volunteers was collected and PBMCs and neutrophils were isolated on Histopaque media. The viability of PBMCs and neutrophils was determined using trypan blue dye exclusion methods. One-way analysis of variance was used in analysing the results. Total viable cell count (TVCC) for PBMCs and neutrophils yielded 298.9 x 104 cells/ml and 327.9 x 104 cells/ml respectively. The TVCC of the highest concentration (100 µg/ml) of the extract used for the treatment of PBMCs was 192.7 x104 cells/ml and that of neutrophil was 50.47 x104 cells/ml equivalent to 82.51% and 61.71% mean viability percentages respectively. The PBMCs and neutrophils treated with 25 µg /ml of the extract have the highest mean percentage viability scores of 94.88% and 74.61% respectively. There was a significant difference in the mean percentage viability when control PBMCs was compared with those treated with 100 µg/ml (p<0.0001) and also when control neutrophils was compared with those treated with 25 µg /ml (p=0 .02), 50 µg /ml and 100 µg /ml (p<0.0001) respectively. The cell viability tends to decrease in a dose-dependent manner. The aqueous leaf extract of C. occidentalis has a potent cytotoxic effect on both cells, especially at a higher dose. The study recommends an in-depth study to improve the credence of the present study findings. Keywords: Cassia occidentalis extract, Cytotoxic activity, Human Neutrophils and PBMCs

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“…The actual percentage yield was 7% w/w and extract was stored at 4C before using it 14 . The crude extract was then dissolved in sterilized distilled water to achieve a dilution of the desired concentration 10,16 . Grouping: Ninety rats were haphazardly splitted into 2 groups Group I: (Control, n= 45): HFD-STZ diabetic rats in this group had normal diet and water available ad libitum and they also received 1ml of 0.3% Carboximethyl cellulose solution every day with intragastric tube for a period of 30 days 14 .…”

Section: Methodsmentioning

confidence: 99%

Outcomes of Cassia Fistula Bark Hexane Extract on Serum Insulin and Glucose Levels in Diabetic Rats

Zahid¹,

Saleem²,

Farooq³

et al. 2022

PJMHS

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Background: Cassia fistula (amaltas) is commonly found in Pakistan and is frequently used in medicines. Its leaves, bark and pods are conventionally used for the treatment of skin disorders, bacterial infection, constipation, colic, cirrhosis, urinary disorders, dysentery, leprosy, rheumatic conditions, jaundice, syphilis, heart diseases and diabetes mellitus (DM). Aim: To determine and compare the outcomes of cassia fistula bark (hexane extract) on serum insulin and blood glucose measures in high-fat diet-fed (HFD) and Streptozotocin-induced type 2 diabetic albino male rats.” Study design: Randomized control trial. Duration and setting: Services Institute of Medical Sciences (SIMS), Lahore from August 2016 to January 2017. Methods: The research was carried out on ninety male albino rats. Rats were induced with diabetes by giving high fat diet for 2 weeks and on fourteenth day, single injection of low dose streptozotocin (25mg/kg). The rats were categorized randomly into two groups, randomly, of 45 animals each. Group 1 was diabetic control (n=45) and group 2 was diabetic experimental and was given hexane extract of Cassia fistula bark (0.45g/kg) in 1ml of 0.3 % Carboximethyl cellulose solution by using intra gastric tube for once in a day for a month. On 31st day, 4-5 ml intra cardiac blood sample was collected. Biochemical parameters serum glucose and insulin were evaluated. Results: The diabetic rats treated with hexane extract of cassia fistula bark, exhibited significant (p=0.05) decrease in serum insulin and glucose levels. Conclusion: Hexane extract of cassia fistula bark possesses potent hypoglycemic effects. Keywords: Cassia fistula, Hypoglycemic effects, diabetic rat

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Effect of Methanolic Extract of <em>Cassia occidentalis</em> L. Root Bark on Body Weight and Selected Biochemical Parameters in Alloxan Induced Diabetic Rats

Cited by 9 publications

References 37 publications

Effects of <i>Senna occidentalis</i> leaf supplement on blood glucose level, liver enzymes and total protein in alloxan-induced diabetic wistar rats

Effects of <i>Senna occidentalis</i> leaf supplement on blood glucose level, liver enzymes and total protein in alloxan-induced diabetic wistar rats

Immunocytotoxic Effect of Aqueous Leaf Extract of Cassia occidentalis on Human Peripheral Blood Mononuclear Cells and Neutrophils

Outcomes of Cassia Fistula Bark Hexane Extract on Serum Insulin and Glucose Levels in Diabetic Rats

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